Culture-independent method for identification of microbial enzyme-encoding genes by activity-based single-cell sequencing using a water-in-oil microdroplet platform - Nakamura, Kazuki; Iizuka, Ryo; Nishi, Shinro; Yoshida, Takao; Hatada, Yuji; Takaki, Yoshihiro; Iguchi, Ayaka; Yoon, Dong Hyun; Sekiguchi, Tetsushi; Shoji, Shuichi; Funatsu, Takashi (Scientific reports, 2016)
Keywords: 14/34; 14/63; 38/23; 631/1647/2217/2220; 631/1647/2234; Bacteria - classification; Bacteria - cytology; Bacteria - genetics; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Bacteriological Techniques; beta-Glucosidase - genetics; beta-Glucosidase - metabolism; Cell culture; Enzymatic activity; Enzymes; Fluorescent Dyes - metabolism; Genetic resources; Genomes; Humanities and Social Sciences; Kinetics; Microscopy, Fluorescence; multidisciplinary; Oils - chemistry; Reproducibility of Results; RNA, Ribosomal, 16S - genetics; Science; Seawater - microbiology; Sequence Analysis, DNA - methods; Single-Cell Analysis - instrumentation; Single-Cell Analysis - methods; Water - chemistry; Water Microbiology - standards