Producing human ceramide-NS by metabolic engineering using yeast Saccharomyces cerevisiae

• Published in: Scientific reports Vol. 5; no. 1; p. 16319
• Main Authors: Murakami, Suguru, Shimamoto, Toshi, Nagano, Hideaki, Tsuruno, Masahiro, Okuhara, Hiroaki, Hatanaka, Haruyo, Tojo, Hiromasa, Kodama, Yukiko, Funato, Kouichi
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 17.11.2015; Nature Publishing Group
• Subjects: 101/58; 45/29; 631/326/2522; 631/61/318; 82/16; Amidohydrolases - genetics; Amidohydrolases - metabolism; Amino Acid Sequence; Antifungal Agents - pharmacology; C gene; Ceramidase; Ceramide; Ceramides - analysis; Ceramides - metabolism; Chromatography, High Pressure Liquid; Depsipeptides - pharmacology; Desaturase; Endoplasmic reticulum; Endoplasmic Reticulum - metabolism; Humanities and Social Sciences; Humans; Industrial Microbiology - methods; Inositol; Localization; Metabolic Engineering; Microscopy, Fluorescence; Mixed Function Oxygenases - deficiency; Mixed Function Oxygenases - genetics; multidisciplinary; Oxidoreductases - genetics; Oxidoreductases - metabolism; Phospholipase C; Saccharomyces cerevisiae - drug effects; Saccharomyces cerevisiae - metabolism; Saccharomyces cerevisiae Proteins - genetics; Saccharomyces cerevisiae Proteins - metabolism; Science; Skin; Sphingolipids; Sphingosine - analysis; Sphingosine - metabolism; Tandem Mass Spectrometry; Type C Phospholipases - genetics; Type C Phospholipases - metabolism; Yeast

Ceramide is one of the most important intercellular components responsible for the barrier and moisture retention functions of the skin. Because of the risks involved with using products of animal origin and the low productivity of plants, the availability of ceramides is currently limited. In this...