Producing human ceramide-NS by metabolic engineering using yeast Saccharomyces cerevisiae

Ceramide is one of the most important intercellular components responsible for the barrier and moisture retention functions of the skin. Because of the risks involved with using products of animal origin and the low productivity of plants, the availability of ceramides is currently limited. In this...

Full description

Saved in:
Bibliographic Details
Published inScientific reports Vol. 5; no. 1; p. 16319
Main Authors Murakami, Suguru, Shimamoto, Toshi, Nagano, Hideaki, Tsuruno, Masahiro, Okuhara, Hiroaki, Hatanaka, Haruyo, Tojo, Hiromasa, Kodama, Yukiko, Funato, Kouichi
Format Journal Article
LanguageEnglish
Published London Nature Publishing Group UK 17.11.2015
Nature Publishing Group
Subjects
101/58
45/29
631/326/2522
631/61/318
82/16
Amidohydrolases - genetics
Amidohydrolases - metabolism
Amino Acid Sequence
Antifungal Agents - pharmacology
C gene
Ceramidase
Ceramide
Ceramides - analysis
Ceramides - metabolism
Chromatography, High Pressure Liquid
Depsipeptides - pharmacology
Desaturase
Endoplasmic reticulum
Endoplasmic Reticulum - metabolism
Humanities and Social Sciences
Humans
Industrial Microbiology - methods
Inositol
Localization
Metabolic Engineering
Microscopy, Fluorescence
Mixed Function Oxygenases - deficiency
Mixed Function Oxygenases - genetics
multidisciplinary
Oxidoreductases - genetics
Oxidoreductases - metabolism
Phospholipase C
Saccharomyces cerevisiae - drug effects
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins - genetics
Saccharomyces cerevisiae Proteins - metabolism
Science
Skin
Sphingolipids
Sphingosine - analysis
Sphingosine - metabolism
Tandem Mass Spectrometry
Type C Phospholipases - genetics
Type C Phospholipases - metabolism
Yeast
Online AccessGet full text

Cover

Abstract Ceramide is one of the most important intercellular components responsible for the barrier and moisture retention functions of the skin. Because of the risks involved with using products of animal origin and the low productivity of plants, the availability of ceramides is currently limited. In this study, we successfully developed a system that produces sphingosine-containing human ceramide-NS in the yeast Saccharomyces cerevisiae by eliminating the genes for yeast sphingolipid hydroxylases (encoded by SUR2 and SCS7 ) and introducing the gene for a human sphingolipid desaturase (encoded by DES1 ). The inactivation of the ceramidase gene YDC1 , overexpression of the inositol phosphosphingolipid phospholipase C gene ISC1 and endoplasmic reticulum localization of the DES1 gene product resulted in enhanced production of ceramide-NS. The engineered yeast strains can serve as hosts not only for providing a sustainable source of ceramide-NS but also for developing further systems to produce sphingosine-containing sphingolipids.
AbstractList Ceramide is one of the most important intercellular components responsible for the barrier and moisture retention functions of the skin. Because of the risks involved with using products of animal origin and the low productivity of plants, the availability of ceramides is currently limited. In this study, we successfully developed a system that produces sphingosine-containing human ceramide-NS in the yeast Saccharomyces cerevisiae by eliminating the genes for yeast sphingolipid hydroxylases (encoded by SUR2 and SCS7) and introducing the gene for a human sphingolipid desaturase (encoded by DES1). The inactivation of the ceramidase gene YDC1, overexpression of the inositol phosphosphingolipid phospholipase C gene ISC1, and endoplasmic reticulum localization of the DES1 gene product resulted in enhanced production of ceramide-NS. The engineered yeast strains can serve as hosts not only for providing a sustainable source of ceramide-NS but also for developing further systems to produce sphingosine-containing sphingolipids.
Abstract Ceramide is one of the most important intercellular components responsible for the barrier and moisture retention functions of the skin. Because of the risks involved with using products of animal origin and the low productivity of plants, the availability of ceramides is currently limited. In this study, we successfully developed a system that produces sphingosine-containing human ceramide-NS in the yeast Saccharomyces cerevisiae by eliminating the genes for yeast sphingolipid hydroxylases (encoded by SUR2 and SCS7 ) and introducing the gene for a human sphingolipid desaturase (encoded by DES1 ). The inactivation of the ceramidase gene YDC1 , overexpression of the inositol phosphosphingolipid phospholipase C gene ISC1 and endoplasmic reticulum localization of the DES1 gene product resulted in enhanced production of ceramide-NS. The engineered yeast strains can serve as hosts not only for providing a sustainable source of ceramide-NS but also for developing further systems to produce sphingosine-containing sphingolipids.
Ceramide is one of the most important intercellular components responsible for the barrier and moisture retention functions of the skin. Because of the risks involved with using products of animal origin and the low productivity of plants, the availability of ceramides is currently limited. In this study, we successfully developed a system that produces sphingosine-containing human ceramide-NS in the yeast Saccharomyces cerevisiae by eliminating the genes for yeast sphingolipid hydroxylases (encoded by SUR2 and SCS7 ) and introducing the gene for a human sphingolipid desaturase (encoded by DES1 ). The inactivation of the ceramidase gene YDC1 , overexpression of the inositol phosphosphingolipid phospholipase C gene ISC1 and endoplasmic reticulum localization of the DES1 gene product resulted in enhanced production of ceramide-NS. The engineered yeast strains can serve as hosts not only for providing a sustainable source of ceramide-NS but also for developing further systems to produce sphingosine-containing sphingolipids.
Ceramide is one of the most important intercellular components responsible for the barrier and moisture retention functions of the skin. Because of the risks involved with using products of animal origin and the low productivity of plants, the availability of ceramides is currently limited. In this study, we successfully developed a system that produces sphingosine-containing human ceramide-NS in the yeast Saccharomyces cerevisiae by eliminating the genes for yeast sphingolipid hydroxylases (encoded by SUR2 and SCS7 ) and introducing the gene for a human sphingolipid desaturase (encoded by DES1 ). The inactivation of the ceramidase gene YDC1 , overexpression of the inositol phosphosphingolipid phospholipase C gene ISC1 , and endoplasmic reticulum localization of the DES1 gene product resulted in enhanced production of ceramide-NS. The engineered yeast strains can serve as hosts not only for providing a sustainable source of ceramide-NS but also for developing further systems to produce sphingosine-containing sphingolipids.
ArticleNumber 16319
Author Okuhara, Hiroaki
Tojo, Hiromasa
Kodama, Yukiko
Funato, Kouichi
Nagano, Hideaki
Hatanaka, Haruyo
Tsuruno, Masahiro
Murakami, Suguru
Shimamoto, Toshi
Author_xml – sequence: 1
  givenname: Suguru
  surname: Murakami
  fullname: Murakami, Suguru
  organization: Department of Biofunctional Science and Technology, Graduate School of Biosphere Science, Hiroshima University
– sequence: 2
  givenname: Toshi
  surname: Shimamoto
  fullname: Shimamoto, Toshi
  organization: Department of Biofunctional Science and Technology, Graduate School of Biosphere Science, Hiroshima University
– sequence: 3
  givenname: Hideaki
  surname: Nagano
  fullname: Nagano, Hideaki
  organization: Suntory World Research Center
– sequence: 4
  givenname: Masahiro
  surname: Tsuruno
  fullname: Tsuruno, Masahiro
  organization: Department of Biofunctional Science and Technology, Graduate School of Biosphere Science, Hiroshima University
– sequence: 5
  givenname: Hiroaki
  surname: Okuhara
  fullname: Okuhara, Hiroaki
  organization: Suntory World Research Center
– sequence: 6
  givenname: Haruyo
  surname: Hatanaka
  fullname: Hatanaka, Haruyo
  organization: Suntory World Research Center
– sequence: 7
  givenname: Hiromasa
  surname: Tojo
  fullname: Tojo, Hiromasa
  organization: Department of Biophysics and Biochemistry, Osaka University Graduate School of Medicine
– sequence: 8
  givenname: Yukiko
  surname: Kodama
  fullname: Kodama, Yukiko
  organization: Suntory World Research Center
– sequence: 9
  givenname: Kouichi
  surname: Funato
  fullname: Funato, Kouichi
  organization: Department of Biofunctional Science and Technology, Graduate School of Biosphere Science, Hiroshima University
BackLink https://www.ncbi.nlm.nih.gov/pubmed/26573460$$D View this record in MEDLINE/PubMed
BookMark eNplkV9P2zAUxa2paJSOB74AirQXQArz3yR-QUIVMCTEJrE97MlynJvWKLGLnSD125PQruqYH-wr-edzr885QhPnHSB0QvAlwaz4FgOsSMaI_ISmFHORUkbpZK8-RMcxPuNhCSo5kZ_RIc1EzniGp-jPz-Cr3li3SJZ9q11iIOjWVpA-PiXlOmmh06VvrEnALawDCCPax3Ffg45d8qSNWerg27WBOD6HVxuthi_ooNZNhOPtOUO_b29-zb-nDz_u7ufXD6kRmHeproUsBdO8rsuaZtzklGNcaZrJXFcSmCgZLSosDOWUGVwWhTRFKeoiyzEVks3Q1UZ31ZctVAZcF3SjVsG2OqyV11b9e-PsUi38q-IZzynOBoGzrUDwLz3ETrU2Gmga7cD3UZGcCTmY997r6wf02ffBDd9TpJCyIHk-GDtD5xvKBB-HeOrdMASrMTO1y2xgT_en35F_ExqAiw0QV6P1EPZa_qf2BrbkouE
CitedBy_id crossref_primary_10_1016_j_cmpb_2020_105436
crossref_primary_10_1016_j_ymben_2020_05_010
crossref_primary_10_1002_bit_26580
crossref_primary_10_1007_s12257_022_0257_8
crossref_primary_10_3389_fimmu_2021_635704
crossref_primary_10_1039_C6SC04831E
crossref_primary_10_3389_fbioe_2021_662979
crossref_primary_10_1016_j_isci_2021_103437
crossref_primary_10_1021_acs_analchem_7b05382
crossref_primary_10_1002_VIW_20220047
crossref_primary_10_1007_s13659_019_0215_0
crossref_primary_10_1111_mmi_13886
crossref_primary_10_15252_embr_201643480
crossref_primary_10_3390_cosmetics7030054
crossref_primary_10_1002_biot_201700052
crossref_primary_10_3390_foods11091286
crossref_primary_10_3390_membranes11120971
crossref_primary_10_1016_j_biotechadv_2021_107696
crossref_primary_10_1021_acs_chemrev_2c00403
crossref_primary_10_1016_j_bbrc_2024_149980
crossref_primary_10_1007_s11356_023_26900_x
Cites_doi 10.1172/JCI200318513
10.1385/ABAB:133:3:203
10.1016/0092-8674(94)90011-6
10.1242/jcs.164160
10.1016/j.biortech.2004.09.019
10.1074/jbc.M110.176875
10.1016/j.jaad.2014.01.891
10.1074/jbc.273.44.28590
10.7150/ijbs.3.120
10.1074/jbc.M003683200
10.1074/jbc.M007721200
10.1016/j.biochi.2009.04.001
10.1111/j.1469-8137.2012.04351.x
10.1074/jbc.M202947200
10.1074/jbc.272.47.29704
10.1083/jcb.200105033
10.1016/S0898-6568(02)00024-4
10.1111/j.1365-2958.2012.08087.x
10.1194/jlr.M800158-JLR200
10.1042/BJ20091589
10.1111/mmi.12056
10.1126/science.8128252
10.1074/jbc.M209925200
10.1091/mbc.e07-08-0740
10.1016/j.bbrc.2011.11.138
10.1016/S0014-5793(04)00274-1
10.1016/j.bbalip.2013.08.006
10.1111/j.1365-2958.2009.06628.x
10.1046/j.1468-3083.2002.00527.x
10.1091/mbc.e08-11-1126
10.1016/j.jaci.2014.05.048
10.1111/j.1365-4632.2008.03687.x
10.1074/jbc.272.15.9809
10.1021/jf061624c
10.1111/1523-1747.ep12470233
10.1038/nbt1222
10.1194/jlr.P030338
10.1002/j.1460-2075.1990.tb07513.x
10.1194/jlr.R800003-JLR200
10.1016/j.febslet.2006.08.039
10.1074/mcp.M400123-MCP200
10.1371/journal.pgen.1002493
10.1016/j.prostaglandins.2007.10.002
10.1016/j.bbamem.2006.06.021
10.1242/jcs.132001
10.1021/bi970091l
10.1124/mol.104.003681
10.1016/0925-4439(93)90135-N
10.1080/00015559850135788
10.1111/j.1365-3164.2012.01090.x
10.4014/jmb.0909.09041
ContentType Journal Article
Copyright The Author(s) 2015
Copyright Nature Publishing Group Nov 2015
Copyright © 2015, Macmillan Publishers Limited 2015 Macmillan Publishers Limited
Copyright_xml – notice: The Author(s) 2015
– notice: Copyright Nature Publishing Group Nov 2015
– notice: Copyright © 2015, Macmillan Publishers Limited 2015 Macmillan Publishers Limited
DBID C6C
CGR
CUY
CVF
ECM
EIF
NPM
AAYXX
CITATION
3V.
7X7
7XB
88A
88E
88I
8FE
8FH
8FI
8FJ
8FK
ABUWG
AFKRA
AZQEC
BBNVY
BENPR
BHPHI
CCPQU
DWQXO
FYUFA
GHDGH
GNUQQ
HCIFZ
K9.
LK8
M0S
M1P
M2P
M7P
PIMPY
PQEST
PQQKQ
PQUKI
PRINS
Q9U
7X8
5PM
DOI 10.1038/srep16319
DatabaseName SpringerOpen
Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
CrossRef
ProQuest Central (Corporate)
Health & Medical Collection
ProQuest Central (purchase pre-March 2016)
Biology Database (Alumni Edition)
Medical Database (Alumni Edition)
Science Database (Alumni Edition)
ProQuest SciTech Collection
ProQuest Natural Science Collection
Hospital Premium Collection
Hospital Premium Collection (Alumni Edition)
ProQuest Central (Alumni) (purchase pre-March 2016)
ProQuest Central (Alumni)
ProQuest Central UK/Ireland
ProQuest Central Essentials
Biological Science Collection
ProQuest Central
Natural Science Collection
ProQuest One Community College
ProQuest Central Korea
Health Research Premium Collection
Health Research Premium Collection (Alumni)
ProQuest Central Student
SciTech Premium Collection
ProQuest Health & Medical Complete (Alumni)
Biological Sciences
Health & Medical Collection (Alumni Edition)
PML(ProQuest Medical Library)
Science Database
Biological Science Database
Publicly Available Content Database
ProQuest One Academic Eastern Edition (DO NOT USE)
ProQuest One Academic
ProQuest One Academic UKI Edition
ProQuest Central China
ProQuest Central Basic
MEDLINE - Academic
PubMed Central (Full Participant titles)
DatabaseTitle MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
CrossRef
Publicly Available Content Database
ProQuest Central Student
ProQuest Central Essentials
ProQuest Health & Medical Complete (Alumni)
ProQuest Central (Alumni Edition)
SciTech Premium Collection
ProQuest One Community College
ProQuest Natural Science Collection
ProQuest Central China
ProQuest Biology Journals (Alumni Edition)
ProQuest Central
Health Research Premium Collection
Health and Medicine Complete (Alumni Edition)
Natural Science Collection
ProQuest Central Korea
Biological Science Collection
ProQuest Medical Library (Alumni)
ProQuest Science Journals (Alumni Edition)
ProQuest Biological Science Collection
ProQuest Central Basic
ProQuest Science Journals
ProQuest One Academic Eastern Edition
ProQuest Hospital Collection
Health Research Premium Collection (Alumni)
Biological Science Database
ProQuest SciTech Collection
ProQuest Hospital Collection (Alumni)
ProQuest Health & Medical Complete
ProQuest Medical Library
ProQuest One Academic UKI Edition
ProQuest One Academic
ProQuest Central (Alumni)
MEDLINE - Academic
DatabaseTitleList Publicly Available Content Database
CrossRef
MEDLINE
MEDLINE - Academic
Database_xml – sequence: 1
  dbid: C6C
  name: SpringerOpen
  url: http://www.springeropen.com/
  sourceTypes: Publisher
– sequence: 2
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 3
  dbid: EIF
  name: MEDLINE
  url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search
  sourceTypes: Index Database
– sequence: 4
  dbid: BENPR
  name: ProQuest Central
  url: https://www.proquest.com/central
  sourceTypes: Aggregation Database
DeliveryMethod fulltext_linktorsrc
Discipline Biology
EISSN 2045-2322
EndPage 16319
ExternalDocumentID 10_1038_srep16319
26573460
Genre Research Support, Non-U.S. Gov't
Journal Article
GroupedDBID 0R~
3V.
4.4
53G
5VS
7X7
88A
88E
88I
8FE
8FH
8FI
8FJ
AAFWJ
AAJSJ
AAKDD
ABDBF
ABUWG
ACGFS
ACSMW
ADBBV
ADRAZ
AENEX
AFKRA
AJTQC
ALIPV
ALMA_UNASSIGNED_HOLDINGS
AOIJS
AZQEC
BAWUL
BBNVY
BCNDV
BENPR
BHPHI
BPHCQ
BVXVI
C6C
CCPQU
DIK
DWQXO
EBD
EBLON
EBS
EJD
ESX
FYUFA
GNUQQ
GROUPED_DOAJ
GX1
HCIFZ
HH5
HMCUK
HYE
KQ8
LK8
M0L
M1P
M2P
M48
M7P
M~E
NAO
OK1
PIMPY
PQQKQ
PROAC
PSQYO
RNT
RNTTT
RPM
SNYQT
UKHRP
CGR
CUY
CVF
ECM
EIF
NPM
AAYXX
AFPKN
CITATION
7XB
8FK
K9.
PQEST
PQUKI
PRINS
Q9U
7X8
5PM
ID FETCH-LOGICAL-c504t-af59b53a4ffbf264c72400da2697ad9e35b328d05c2423c0b889c8b5f86702593
IEDL.DBID RPM
ISSN 2045-2322
IngestDate Tue Sep 17 21:11:13 EDT 2024
Fri Oct 25 23:57:38 EDT 2024
Thu Oct 10 20:52:17 EDT 2024
Fri Aug 23 03:28:43 EDT 2024
Tue Oct 15 23:54:57 EDT 2024
Fri Oct 11 20:47:26 EDT 2024
IsDoiOpenAccess true
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 1
Language English
License This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c504t-af59b53a4ffbf264c72400da2697ad9e35b328d05c2423c0b889c8b5f86702593
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
These authors contributed equally to this work.
OpenAccessLink https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4647206/
PMID 26573460
PQID 1899817757
PQPubID 2041939
PageCount 1
ParticipantIDs pubmedcentral_primary_oai_pubmedcentral_nih_gov_4647206
proquest_miscellaneous_1735900559
proquest_journals_1899817757
crossref_primary_10_1038_srep16319
pubmed_primary_26573460
springer_journals_10_1038_srep16319
PublicationCentury 2000
PublicationDate 2015-11-17
PublicationDateYYYYMMDD 2015-11-17
PublicationDate_xml – month: 11
  year: 2015
  text: 2015-11-17
  day: 17
PublicationDecade 2010
PublicationPlace London
PublicationPlace_xml – name: London
– name: England
PublicationTitle Scientific reports
PublicationTitleAbbrev Sci Rep
PublicationTitleAlternate Sci Rep
PublicationYear 2015
Publisher Nature Publishing Group UK
Nature Publishing Group
Publisher_xml – name: Nature Publishing Group UK
– name: Nature Publishing Group
References Vionnet, Roubaty, Ejsing, Knudsen, Conzelmann (CR44) 2011; 286
Mao, Xu, Bielawska, Szulc, Obeid (CR26) 2000; 275
Funato, Lombardi, Vallee, Riezman (CR50) 2003; 278
Kajiwara (CR30) 2012; 86
Natter (CR38) 2005; 4
Cadena, Kurten, Gill (CR36) 1997; 36
Elias, Wakefield (CR7) 2014; 134
Ikeda (CR33) 2015; 128
Nagiec (CR25) 1997; 272
Huang, Withers, Dickson (CR42) 2014; 1841
Bouwstra, Ponec (CR1) 2006; 1758
Takagi (CR12) 2003; 112
Letourneur (CR41) 1994; 79
Mizutani, Kihara, Igarashi (CR34) 2004; 563
Jackson, Nilsson, Peterson (CR39) 1990; 9
Sawai (CR27) 2000; 275
Mizutani, Mitsutake, Tsuji, Kihara, Igarashi (CR3) 2009; 91
Matsuyama (CR37) 2006; 24
Li (CR14) 2007; 3
Kwun, Lee, Rho, Yun (CR15) 2006; 133
Rao, Acharya (CR21) 2008; 85
Kim, Noh, Koo, Yun (CR16) 2010; 20
Idkowiak-Baldys (CR35) 2010; 427
Takakuwa, Saito, Ohnishi, Oda (CR17) 2005; 96
Cerantola (CR31) 2009; 71
Haak, Gable, Beeler, Dunn (CR22) 1997; 272
Sperling, Zahringer, Heinz (CR48) 1998; 273
Holleran, Takagi, Uchida (CR2) 2006; 580
Wang, Wang, Fehr (CR18) 2006; 54
Tani, Kuge (CR28) 2012; 417
Mizutani, Kihara, Chiba, Tojo, Igarashi (CR49) 2008; 49
Guan (CR32) 2009; 20
Nishifuji, Yoon (CR4) 2013; 24
Cosson, Letourneur (CR40) 1994; 263
Triola (CR47) 2004; 66
De Paepe, Roseeuw, Rogiers (CR9) 2002; 16
Huang, Liu, Dickson (CR43) 2012; 8
Epstein, Castillon, Qin, Riezman (CR29) 2012; 84
Motta (CR51) 1993; 1182
Funato, Riezman (CR24) 2001; 155
Kajiwara (CR46) 2014; 127
Kim (CR8) 2002; 14
Huang, Chang (CR10) 2008; 47
Imokawa (CR5) 1991; 96
Dickson (CR23) 2008; 49
Kajiwara (CR45) 2008; 19
Meckfessel, Brandt (CR11) 2014; 71
Di Nardo, Wertz, Giannetti, Seidenari (CR6) 1998; 78
Ternes, Franke, Zahringer, Sperling, Heinz (CR20) 2002; 277
Janssens (CR13) 2012; 53
Konig (CR19) 2012; 196
20105137 - Biochem J. 2010 Apr 15;427(2):265-74
25131691 - J Allergy Clin Immunol. 2014 Oct;134(4):781-791.e1
8128252 - Science. 1994 Mar 18;263(5153):1629-31
17311087 - Int J Biol Sci. 2007;3(2):120-8
10900202 - J Biol Chem. 2000 Oct 6;275(40):31369-78
9368039 - J Biol Chem. 1997 Nov 21;272(47):29704-10
2120038 - EMBO J. 1990 Oct;9(10):3153-62
15063729 - FEBS Lett. 2004 Apr 9;563(1-3):93-7
18717861 - Int J Dermatol. 2008 Aug;47(8):812-9
23954556 - Biochim Biophys Acta. 2014 May;1841(5):657-64
16823372 - Nat Biotechnol. 2006 Jul;24(7):841-7
9092515 - J Biol Chem. 1997 Apr 11;272(15):9809-17
18287539 - Mol Biol Cell. 2008 May;19(5):2069-82
23062268 - Mol Microbiol. 2012 Dec;86(5):1246-61
16720901 - Appl Biochem Biotechnol. 2006 Jun;133(3):203-10
15716577 - Mol Cell Proteomics. 2005 May;4(5):662-72
9786850 - J Biol Chem. 1998 Oct 30;273(44):28590-6
12482041 - J Eur Acad Dermatol Venereol. 2002 Nov;16(6):587-94
26045446 - J Cell Sci. 2015 Jul 15;128(14):2454-67
20208441 - J Microbiol Biotechnol. 2010 Feb;20(2):356-62
16962101 - FEBS Lett. 2006 Oct 9;580(23):5456-66
16945325 - Biochim Biophys Acta. 2006 Dec;1758(12):2080-95
11006294 - J Biol Chem. 2000 Dec 15;275(50):39793-8
24656726 - J Am Acad Dermatol. 2014 Jul;71(1):177-84
11733544 - J Cell Biol. 2001 Dec 10;155(6):949-59
22319457 - PLoS Genet. 2012 Feb;8(2):e1002493
11937514 - J Biol Chem. 2002 Jul 12;277(28):25512-8
8357845 - Biochim Biophys Acta. 1993 Sep 8;1182(2):147-51
22616608 - Mol Microbiol. 2012 Jun;84(6):1018-32
21173150 - J Biol Chem. 2011 Feb 25;286(8):6769-79
8001155 - Cell. 1994 Dec 30;79(7):1199-207
18541923 - J Lipid Res. 2008 Nov;49(11):2356-64
9498022 - Acta Derm Venereol. 1998 Jan;78(1):27-30
14597763 - J Clin Invest. 2003 Nov;112(9):1372-82
15371559 - Mol Pharmacol. 2004 Dec;66(6):1671-8
23331681 - Vet Dermatol. 2013 Feb;24(1):60-72.e15-6
19225153 - Mol Biol Cell. 2009 Apr;20(7):2083-95
9188692 - Biochemistry. 1997 Jun 10;36(23):6960-7
19210614 - Mol Microbiol. 2009 Mar;71(6):1523-37
24213531 - J Cell Sci. 2014 Jan 15;127(Pt 2):376-87
18296751 - J Lipid Res. 2008 May;49(5):909-21
22166213 - Biochem Biophys Res Commun. 2012 Jan 13;417(2):673-8
12034359 - Cell Signal. 2002 Sep;14(9):779-85
15668206 - Bioresour Technol. 2005 Jun;96(9):1089-92
2007790 - J Invest Dermatol. 1991 Apr;96(4):523-6
12493772 - J Biol Chem. 2003 Feb 28;278(9):7325-34
18035569 - Prostaglandins Other Lipid Mediat. 2008 Feb;85(1-2):1-16
17002403 - J Agric Food Chem. 2006 Oct 4;54(20):7422-8
19364519 - Biochimie. 2009 Jun;91(6):784-90
23025549 - New Phytol. 2012 Dec;196(4):1086-97
23024286 - J Lipid Res. 2012 Dec;53(12):2755-66
H Sawai (BFsrep16319_CR27) 2000; 275
N Takakuwa (BFsrep16319_CR17) 2005; 96
X Huang (BFsrep16319_CR42) 2014; 1841
X Huang (BFsrep16319_CR43) 2012; 8
V Cerantola (BFsrep16319_CR31) 2009; 71
Y Mizutani (BFsrep16319_CR34) 2004; 563
P Ternes (BFsrep16319_CR20) 2002; 277
M Tani (BFsrep16319_CR28) 2012; 417
MR Jackson (BFsrep16319_CR39) 1990; 9
P Cosson (BFsrep16319_CR40) 1994; 263
K Kajiwara (BFsrep16319_CR45) 2008; 19
DS Kim (BFsrep16319_CR8) 2002; 14
S Konig (BFsrep16319_CR19) 2012; 196
D Haak (BFsrep16319_CR22) 1997; 272
SK Kim (BFsrep16319_CR16) 2010; 20
C Mao (BFsrep16319_CR26) 2000; 275
K Kajiwara (BFsrep16319_CR46) 2014; 127
F Letourneur (BFsrep16319_CR41) 1994; 79
XL Guan (BFsrep16319_CR32) 2009; 20
RP Rao (BFsrep16319_CR21) 2008; 85
S Epstein (BFsrep16319_CR29) 2012; 84
DL Cadena (BFsrep16319_CR36) 1997; 36
G Triola (BFsrep16319_CR47) 2004; 66
S Motta (BFsrep16319_CR51) 1993; 1182
K Funato (BFsrep16319_CR24) 2001; 155
L Wang (BFsrep16319_CR18) 2006; 54
J Idkowiak-Baldys (BFsrep16319_CR35) 2010; 427
WM Holleran (BFsrep16319_CR2) 2006; 580
A Di Nardo (BFsrep16319_CR6) 1998; 78
K Natter (BFsrep16319_CR38) 2005; 4
RC Dickson (BFsrep16319_CR23) 2008; 49
W Li (BFsrep16319_CR14) 2007; 3
KH Kwun (BFsrep16319_CR15) 2006; 133
Y Mizutani (BFsrep16319_CR49) 2008; 49
K Funato (BFsrep16319_CR50) 2003; 278
K Nishifuji (BFsrep16319_CR4) 2013; 24
HC Huang (BFsrep16319_CR10) 2008; 47
S Takagi (BFsrep16319_CR12) 2003; 112
M Janssens (BFsrep16319_CR13) 2012; 53
C Vionnet (BFsrep16319_CR44) 2011; 286
K Kajiwara (BFsrep16319_CR30) 2012; 86
A Ikeda (BFsrep16319_CR33) 2015; 128
JA Bouwstra (BFsrep16319_CR1) 2006; 1758
MH Meckfessel (BFsrep16319_CR11) 2014; 71
PM Elias (BFsrep16319_CR7) 2014; 134
A Matsuyama (BFsrep16319_CR37) 2006; 24
Y Mizutani (BFsrep16319_CR3) 2009; 91
P Sperling (BFsrep16319_CR48) 1998; 273
MM Nagiec (BFsrep16319_CR25) 1997; 272
G Imokawa (BFsrep16319_CR5) 1991; 96
K De Paepe (BFsrep16319_CR9) 2002; 16
References_xml – volume: 112
  start-page: 1372
  year: 2003
  end-page: 1382
  ident: CR12
  article-title: Alteration of the 4-sphingenine scaffolds of ceramides in keratinocyte-specific Arnt-deficient mice affects skin barrier function
  publication-title: J Clin Invest
  doi: 10.1172/JCI200318513
  contributor:
    fullname: Takagi
– volume: 133
  start-page: 203
  year: 2006
  end-page: 210
  ident: CR15
  article-title: Production of ceramide with Saccharomyces cerevisiae
  publication-title: Appl Biochem Biotechnol
  doi: 10.1385/ABAB:133:3:203
  contributor:
    fullname: Yun
– volume: 79
  start-page: 1199
  year: 1994
  end-page: 1207
  ident: CR41
  article-title: Coatomer is essential for retrieval of dilysine-tagged proteins to the endoplasmic reticulum
  publication-title: Cell
  doi: 10.1016/0092-8674(94)90011-6
  contributor:
    fullname: Letourneur
– volume: 128
  start-page: 2454
  year: 2015
  end-page: 2467
  ident: CR33
  article-title: Sphingolipids regulate telomere clustering by affecting transcriptional levels of genes involved in telomere homeostasis
  publication-title: J Cell Sci
  doi: 10.1242/jcs.164160
  contributor:
    fullname: Ikeda
– volume: 96
  start-page: 1089
  year: 2005
  end-page: 1092
  ident: CR17
  article-title: Determination of glucosylceramide contents in crop tissues and by-products from their processing
  publication-title: Bioresour Technol
  doi: 10.1016/j.biortech.2004.09.019
  contributor:
    fullname: Oda
– volume: 286
  start-page: 6769
  year: 2011
  end-page: 6779
  ident: CR44
  article-title: Yeast cells lacking all known ceramide synthases continue to make complex sphingolipids and to incorporate ceramides into glycosylphosphatidylinositol (GPI) anchors
  publication-title: J Biol Chem
  doi: 10.1074/jbc.M110.176875
  contributor:
    fullname: Conzelmann
– volume: 71
  start-page: 177
  year: 2014
  end-page: 184
  ident: CR11
  article-title: The structure, function and importance of ceramides in skin and their use as therapeutic agents in skin-care products
  publication-title: J Am Acad Dermatol
  doi: 10.1016/j.jaad.2014.01.891
  contributor:
    fullname: Brandt
– volume: 273
  start-page: 28590
  year: 1998
  end-page: 28596
  ident: CR48
  article-title: A sphingolipid desaturase from higher plants. Identification of a new cytochrome b5 fusion protein
  publication-title: J Biol Chem
  doi: 10.1074/jbc.273.44.28590
  contributor:
    fullname: Heinz
– volume: 3
  start-page: 120
  year: 2007
  end-page: 128
  ident: CR14
  article-title: Depletion of ceramides with very long chain fatty acids causes defective skin permeability barrier function and neonatal lethality in ELOVL4 deficient mice
  publication-title: Int J Biol Sci
  doi: 10.7150/ijbs.3.120
  contributor:
    fullname: Li
– volume: 275
  start-page: 31369
  year: 2000
  end-page: 31378
  ident: CR26
  article-title: Cloning and characterization of a Saccharomyces cerevisiae alkaline ceramidase with specificity for dihydroceramide
  publication-title: J Biol Chem
  doi: 10.1074/jbc.M003683200
  contributor:
    fullname: Obeid
– volume: 275
  start-page: 39793
  year: 2000
  end-page: 39798
  ident: CR27
  article-title: Identification of ISC1 (YER019w) as inositol phosphosphingolipid phospholipase C in Saccharomyces cerevisiae
  publication-title: J Biol Chem
  doi: 10.1074/jbc.M007721200
  contributor:
    fullname: Sawai
– volume: 91
  start-page: 784
  year: 2009
  end-page: 790
  ident: CR3
  article-title: Ceramide biosynthesis in keratinocyte and its role in skin function
  publication-title: Biochimie
  doi: 10.1016/j.biochi.2009.04.001
  contributor:
    fullname: Igarashi
– volume: 196
  start-page: 1086
  year: 2012
  end-page: 1097
  ident: CR19
  article-title: Arabidopsis mutants of sphingolipid fatty acid alpha-hydroxylases accumulate ceramides and salicylates
  publication-title: New Phytol
  doi: 10.1111/j.1469-8137.2012.04351.x
  contributor:
    fullname: Konig
– volume: 277
  start-page: 25512
  year: 2002
  end-page: 25518
  ident: CR20
  article-title: Identification and characterization of a sphingolipid delta 4-desaturase family
  publication-title: J Biol Chem
  doi: 10.1074/jbc.M202947200
  contributor:
    fullname: Heinz
– volume: 272
  start-page: 29704
  year: 1997
  end-page: 29710
  ident: CR22
  article-title: Hydroxylation of Saccharomyces cerevisiae ceramides requires Sur2p and Scs7p
  publication-title: J Biol Chem
  doi: 10.1074/jbc.272.47.29704
  contributor:
    fullname: Dunn
– volume: 155
  start-page: 949
  year: 2001
  end-page: 959
  ident: CR24
  article-title: Vesicular and nonvesicular transport of ceramide from ER to the Golgi apparatus in yeast
  publication-title: J Cell Biol
  doi: 10.1083/jcb.200105033
  contributor:
    fullname: Riezman
– volume: 14
  start-page: 779
  year: 2002
  end-page: 785
  ident: CR8
  article-title: Delayed ERK activation by ceramide reduces melanin synthesis in human melanocytes
  publication-title: Cell Signal
  doi: 10.1016/S0898-6568(02)00024-4
  contributor:
    fullname: Kim
– volume: 84
  start-page: 1018
  year: 2012
  end-page: 1032
  ident: CR29
  article-title: An essential function of sphingolipids in yeast cell division
  publication-title: Mol Microbiol
  doi: 10.1111/j.1365-2958.2012.08087.x
  contributor:
    fullname: Riezman
– volume: 49
  start-page: 2356
  year: 2008
  end-page: 2364
  ident: CR49
  article-title: 2-Hydroxy-ceramide synthesis by ceramide synthase family: enzymatic basis for the preference of FA chain length
  publication-title: J Lipid Res
  doi: 10.1194/jlr.M800158-JLR200
  contributor:
    fullname: Igarashi
– volume: 427
  start-page: 265
  year: 2010
  end-page: 274
  ident: CR35
  article-title: Dihydroceramide desaturase activity is modulated by oxidative stress
  publication-title: Biochem J
  doi: 10.1042/BJ20091589
  contributor:
    fullname: Idkowiak-Baldys
– volume: 86
  start-page: 1246
  year: 2012
  end-page: 1261
  ident: CR30
  article-title: Perturbation of sphingolipid metabolism induces endoplasmic reticulum stress-mediated mitochondrial apoptosis in budding yeast
  publication-title: Mol Microbiol
  doi: 10.1111/mmi.12056
  contributor:
    fullname: Kajiwara
– volume: 263
  start-page: 1629
  year: 1994
  end-page: 1631
  ident: CR40
  article-title: Coatomer interaction with di-lysine endoplasmic reticulum retention motifs
  publication-title: Science
  doi: 10.1126/science.8128252
  contributor:
    fullname: Letourneur
– volume: 278
  start-page: 7325
  year: 2003
  end-page: 7334
  ident: CR50
  article-title: Lcb4p is a key regulator of ceramide synthesis from exogenous long chain sphingoid base in Saccharomyces cerevisiae
  publication-title: J Biol Chem
  doi: 10.1074/jbc.M209925200
  contributor:
    fullname: Riezman
– volume: 19
  start-page: 2069
  year: 2008
  end-page: 2082
  ident: CR45
  article-title: Yeast ARV1 is required for efficient delivery of an early GPI intermediate to the first mannosyltransferase during GPI assembly and controls lipid flow from the endoplasmic reticulum
  publication-title: Mol Biol Cell
  doi: 10.1091/mbc.e07-08-0740
  contributor:
    fullname: Kajiwara
– volume: 417
  start-page: 673
  year: 2012
  end-page: 678
  ident: CR28
  article-title: Hydroxylation state of fatty acid and long-chain base moieties of sphingolipid determine the sensitivity to growth inhibition due to AUR1 repression in Saccharomyces cerevisiae
  publication-title: Biochem Biophys Res Commun
  doi: 10.1016/j.bbrc.2011.11.138
  contributor:
    fullname: Kuge
– volume: 563
  start-page: 93
  year: 2004
  end-page: 97
  ident: CR34
  article-title: Identification of the human sphingolipid C4-hydroxylase, hDES2 and its up-regulation during keratinocyte differentiation
  publication-title: FEBS Lett
  doi: 10.1016/S0014-5793(04)00274-1
  contributor:
    fullname: Igarashi
– volume: 1841
  start-page: 657
  year: 2014
  end-page: 664
  ident: CR42
  article-title: Sphingolipids and lifespan regulation
  publication-title: Biochim Biophys Acta
  doi: 10.1016/j.bbalip.2013.08.006
  contributor:
    fullname: Dickson
– volume: 20
  start-page: 356
  year: 2010
  end-page: 362
  ident: CR16
  article-title: Effect of expression of genes in the sphingolipid synthesis pathway on the biosynthesis of ceramide in Saccharomyces cerevisiae
  publication-title: J Microbiol Biotechnol
  contributor:
    fullname: Yun
– volume: 71
  start-page: 1523
  year: 2009
  end-page: 1537
  ident: CR31
  article-title: Aureobasidin A arrests growth of yeast cells through both ceramide intoxication and deprivation of essential inositolphosphorylceramides
  publication-title: Mol Microbiol
  doi: 10.1111/j.1365-2958.2009.06628.x
  contributor:
    fullname: Cerantola
– volume: 16
  start-page: 587
  year: 2002
  end-page: 594
  ident: CR9
  article-title: Repair of acetone- and sodium lauryl sulphate-damaged human skin barrier function using topically applied emulsions containing barrier lipids
  publication-title: J Eur Acad Dermatol Venereol
  doi: 10.1046/j.1468-3083.2002.00527.x
  contributor:
    fullname: Rogiers
– volume: 20
  start-page: 2083
  year: 2009
  end-page: 2095
  ident: CR32
  article-title: Functional interactions between sphingolipids and sterols in biological membranes regulating cell physiology
  publication-title: Mol Biol Cell
  doi: 10.1091/mbc.e08-11-1126
  contributor:
    fullname: Guan
– volume: 134
  start-page: 781
  year: 2014
  end-page: 791
  ident: CR7
  article-title: Mechanisms of abnormal lamellar body secretion and the dysfunctional skin barrier in patients with atopic dermatitis
  publication-title: J Allergy Clin Immunol
  doi: 10.1016/j.jaci.2014.05.048
  contributor:
    fullname: Wakefield
– volume: 47
  start-page: 812
  year: 2008
  end-page: 819
  ident: CR10
  article-title: Ceramide 1 and ceramide 3 act synergistically on skin hydration and the transepidermal water loss of sodium lauryl sulfate-irritated skin
  publication-title: Int J Dermatol
  doi: 10.1111/j.1365-4632.2008.03687.x
  contributor:
    fullname: Chang
– volume: 272
  start-page: 9809
  year: 1997
  end-page: 9817
  ident: CR25
  article-title: Sphingolipid synthesis as a target for antifungal drugs. Complementation of the inositol phosphorylceramide synthase defect in a mutant strain of Saccharomyces cerevisiae by the AUR1 gene
  publication-title: J Biol Chem
  doi: 10.1074/jbc.272.15.9809
  contributor:
    fullname: Nagiec
– volume: 54
  start-page: 7422
  year: 2006
  end-page: 7428
  ident: CR18
  article-title: HPLC quantification of sphingolipids in soybeans with modified palmitate content
  publication-title: J Agric Food Chem
  doi: 10.1021/jf061624c
  contributor:
    fullname: Fehr
– volume: 96
  start-page: 523
  year: 1991
  end-page: 526
  ident: CR5
  article-title: Decreased level of ceramides in stratum corneum of atopic dermatitis: an etiologic factor in atopic dry skin?
  publication-title: J Invest Dermatol
  doi: 10.1111/1523-1747.ep12470233
  contributor:
    fullname: Imokawa
– volume: 24
  start-page: 841
  year: 2006
  end-page: 847
  ident: CR37
  article-title: ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe
  publication-title: Nat Biotechnol
  doi: 10.1038/nbt1222
  contributor:
    fullname: Matsuyama
– volume: 53
  start-page: 2755
  year: 2012
  end-page: 2766
  ident: CR13
  article-title: Increase in short-chain ceramides correlates with an altered lipid organization and decreased barrier function in atopic eczema patients
  publication-title: J Lipid Res
  doi: 10.1194/jlr.P030338
  contributor:
    fullname: Janssens
– volume: 9
  start-page: 3153
  year: 1990
  end-page: 3162
  ident: CR39
  article-title: Identification of a consensus motif for retention of transmembrane proteins in the endoplasmic reticulum
  publication-title: Embo j
  doi: 10.1002/j.1460-2075.1990.tb07513.x
  contributor:
    fullname: Peterson
– volume: 49
  start-page: 909
  year: 2008
  end-page: 921
  ident: CR23
  article-title: Thematic review series: sphingolipids. New insights into sphingolipid metabolism and function in budding yeast
  publication-title: J Lipid Res
  doi: 10.1194/jlr.R800003-JLR200
  contributor:
    fullname: Dickson
– volume: 580
  start-page: 5456
  year: 2006
  end-page: 5466
  ident: CR2
  article-title: Epidermal sphingolipids: metabolism, function and roles in skin disorders
  publication-title: FEBS Lett
  doi: 10.1016/j.febslet.2006.08.039
  contributor:
    fullname: Uchida
– volume: 4
  start-page: 662
  year: 2005
  end-page: 672
  ident: CR38
  article-title: The spatial organization of lipid synthesis in the yeast Saccharomyces cerevisiae derived from large scale green fluorescent protein tagging and high resolution microscopy
  publication-title: Mol Cell Proteomics
  doi: 10.1074/mcp.M400123-MCP200
  contributor:
    fullname: Natter
– volume: 8
  start-page: e1002493
  year: 2012
  ident: CR43
  article-title: Down-regulating sphingolipid synthesis increases yeast lifespan
  publication-title: PLoS Genet
  doi: 10.1371/journal.pgen.1002493
  contributor:
    fullname: Dickson
– volume: 85
  start-page: 1
  year: 2008
  end-page: 16
  ident: CR21
  article-title: Sphingolipids and membrane biology as determined from genetic models
  publication-title: Prostaglandins Other Lipid Mediat
  doi: 10.1016/j.prostaglandins.2007.10.002
  contributor:
    fullname: Acharya
– volume: 1758
  start-page: 2080
  year: 2006
  end-page: 2095
  ident: CR1
  article-title: The skin barrier in healthy and diseased state
  publication-title: Biochim Biophys Acta
  doi: 10.1016/j.bbamem.2006.06.021
  contributor:
    fullname: Ponec
– volume: 127
  start-page: 376
  year: 2014
  end-page: 387
  ident: CR46
  article-title: Osh proteins regulate COPII-mediated vesicular transport of ceramide from the endoplasmic reticulum in budding yeast
  publication-title: J Cell Sci
  doi: 10.1242/jcs.132001
  contributor:
    fullname: Kajiwara
– volume: 36
  start-page: 6960
  year: 1997
  end-page: 6967
  ident: CR36
  article-title: The product of the MLD gene is a member of the membrane fatty acid desaturase family: overexpression of MLD inhibits EGF receptor biosynthesis
  publication-title: Biochemistry
  doi: 10.1021/bi970091l
  contributor:
    fullname: Gill
– volume: 66
  start-page: 1671
  year: 2004
  end-page: 1678
  ident: CR47
  article-title: Specificity of the dihydroceramide desaturase inhibitor N-[(1R,2S)-2-hydroxy-1-hydroxymethyl-2-(2-tridecyl-1-cyclopropenyl)ethyl]octanami de (GT11) in primary cultured cerebellar neurons
  publication-title: Mol Pharmacol
  doi: 10.1124/mol.104.003681
  contributor:
    fullname: Triola
– volume: 1182
  start-page: 147
  year: 1993
  end-page: 151
  ident: CR51
  article-title: Ceramide composition of the psoriatic scale
  publication-title: Biochim Biophys Acta
  doi: 10.1016/0925-4439(93)90135-N
  contributor:
    fullname: Motta
– volume: 78
  start-page: 27
  year: 1998
  end-page: 30
  ident: CR6
  article-title: Ceramide and cholesterol composition of the skin of patients with atopic dermatitis
  publication-title: Acta Derm Venereol
  doi: 10.1080/00015559850135788
  contributor:
    fullname: Seidenari
– volume: 24
  start-page: 60
  year: 2013
  end-page: 72
  ident: CR4
  article-title: The stratum corneum: the rampart of the mammalian body
  publication-title: Vet Dermatol
  doi: 10.1111/j.1365-3164.2012.01090.x
  contributor:
    fullname: Yoon
– volume: 53
  start-page: 2755
  year: 2012
  ident: BFsrep16319_CR13
  publication-title: J Lipid Res
  doi: 10.1194/jlr.P030338
  contributor:
    fullname: M Janssens
– volume: 14
  start-page: 779
  year: 2002
  ident: BFsrep16319_CR8
  publication-title: Cell Signal
  doi: 10.1016/S0898-6568(02)00024-4
  contributor:
    fullname: DS Kim
– volume: 417
  start-page: 673
  year: 2012
  ident: BFsrep16319_CR28
  publication-title: Biochem Biophys Res Commun
  doi: 10.1016/j.bbrc.2011.11.138
  contributor:
    fullname: M Tani
– volume: 20
  start-page: 2083
  year: 2009
  ident: BFsrep16319_CR32
  publication-title: Mol Biol Cell
  doi: 10.1091/mbc.e08-11-1126
  contributor:
    fullname: XL Guan
– volume: 47
  start-page: 812
  year: 2008
  ident: BFsrep16319_CR10
  publication-title: Int J Dermatol
  doi: 10.1111/j.1365-4632.2008.03687.x
  contributor:
    fullname: HC Huang
– volume: 96
  start-page: 1089
  year: 2005
  ident: BFsrep16319_CR17
  publication-title: Bioresour Technol
  doi: 10.1016/j.biortech.2004.09.019
  contributor:
    fullname: N Takakuwa
– volume: 24
  start-page: 60
  year: 2013
  ident: BFsrep16319_CR4
  publication-title: Vet Dermatol
  doi: 10.1111/j.1365-3164.2012.01090.x
  contributor:
    fullname: K Nishifuji
– volume: 54
  start-page: 7422
  year: 2006
  ident: BFsrep16319_CR18
  publication-title: J Agric Food Chem
  doi: 10.1021/jf061624c
  contributor:
    fullname: L Wang
– volume: 79
  start-page: 1199
  year: 1994
  ident: BFsrep16319_CR41
  publication-title: Cell
  doi: 10.1016/0092-8674(94)90011-6
  contributor:
    fullname: F Letourneur
– volume: 8
  start-page: e1002493
  year: 2012
  ident: BFsrep16319_CR43
  publication-title: PLoS Genet
  doi: 10.1371/journal.pgen.1002493
  contributor:
    fullname: X Huang
– volume: 278
  start-page: 7325
  year: 2003
  ident: BFsrep16319_CR50
  publication-title: J Biol Chem
  doi: 10.1074/jbc.M209925200
  contributor:
    fullname: K Funato
– volume: 85
  start-page: 1
  year: 2008
  ident: BFsrep16319_CR21
  publication-title: Prostaglandins Other Lipid Mediat
  doi: 10.1016/j.prostaglandins.2007.10.002
  contributor:
    fullname: RP Rao
– volume: 155
  start-page: 949
  year: 2001
  ident: BFsrep16319_CR24
  publication-title: J Cell Biol
  doi: 10.1083/jcb.200105033
  contributor:
    fullname: K Funato
– volume: 272
  start-page: 9809
  year: 1997
  ident: BFsrep16319_CR25
  publication-title: J Biol Chem
  doi: 10.1074/jbc.272.15.9809
  contributor:
    fullname: MM Nagiec
– volume: 580
  start-page: 5456
  year: 2006
  ident: BFsrep16319_CR2
  publication-title: FEBS Lett
  doi: 10.1016/j.febslet.2006.08.039
  contributor:
    fullname: WM Holleran
– volume: 3
  start-page: 120
  year: 2007
  ident: BFsrep16319_CR14
  publication-title: Int J Biol Sci
  doi: 10.7150/ijbs.3.120
  contributor:
    fullname: W Li
– volume: 273
  start-page: 28590
  year: 1998
  ident: BFsrep16319_CR48
  publication-title: J Biol Chem
  doi: 10.1074/jbc.273.44.28590
  contributor:
    fullname: P Sperling
– volume: 275
  start-page: 31369
  year: 2000
  ident: BFsrep16319_CR26
  publication-title: J Biol Chem
  doi: 10.1074/jbc.M003683200
  contributor:
    fullname: C Mao
– volume: 16
  start-page: 587
  year: 2002
  ident: BFsrep16319_CR9
  publication-title: J Eur Acad Dermatol Venereol
  doi: 10.1046/j.1468-3083.2002.00527.x
  contributor:
    fullname: K De Paepe
– volume: 84
  start-page: 1018
  year: 2012
  ident: BFsrep16319_CR29
  publication-title: Mol Microbiol
  doi: 10.1111/j.1365-2958.2012.08087.x
  contributor:
    fullname: S Epstein
– volume: 49
  start-page: 2356
  year: 2008
  ident: BFsrep16319_CR49
  publication-title: J Lipid Res
  doi: 10.1194/jlr.M800158-JLR200
  contributor:
    fullname: Y Mizutani
– volume: 427
  start-page: 265
  year: 2010
  ident: BFsrep16319_CR35
  publication-title: Biochem J
  doi: 10.1042/BJ20091589
  contributor:
    fullname: J Idkowiak-Baldys
– volume: 133
  start-page: 203
  year: 2006
  ident: BFsrep16319_CR15
  publication-title: Appl Biochem Biotechnol
  doi: 10.1385/ABAB:133:3:203
  contributor:
    fullname: KH Kwun
– volume: 78
  start-page: 27
  year: 1998
  ident: BFsrep16319_CR6
  publication-title: Acta Derm Venereol
  doi: 10.1080/00015559850135788
  contributor:
    fullname: A Di Nardo
– volume: 112
  start-page: 1372
  year: 2003
  ident: BFsrep16319_CR12
  publication-title: J Clin Invest
  doi: 10.1172/JCI200318513
  contributor:
    fullname: S Takagi
– volume: 128
  start-page: 2454
  year: 2015
  ident: BFsrep16319_CR33
  publication-title: J Cell Sci
  doi: 10.1242/jcs.164160
  contributor:
    fullname: A Ikeda
– volume: 127
  start-page: 376
  year: 2014
  ident: BFsrep16319_CR46
  publication-title: J Cell Sci
  doi: 10.1242/jcs.132001
  contributor:
    fullname: K Kajiwara
– volume: 1182
  start-page: 147
  year: 1993
  ident: BFsrep16319_CR51
  publication-title: Biochim Biophys Acta
  doi: 10.1016/0925-4439(93)90135-N
  contributor:
    fullname: S Motta
– volume: 134
  start-page: 781
  year: 2014
  ident: BFsrep16319_CR7
  publication-title: J Allergy Clin Immunol
  doi: 10.1016/j.jaci.2014.05.048
  contributor:
    fullname: PM Elias
– volume: 275
  start-page: 39793
  year: 2000
  ident: BFsrep16319_CR27
  publication-title: J Biol Chem
  doi: 10.1074/jbc.M007721200
  contributor:
    fullname: H Sawai
– volume: 71
  start-page: 1523
  year: 2009
  ident: BFsrep16319_CR31
  publication-title: Mol Microbiol
  doi: 10.1111/j.1365-2958.2009.06628.x
  contributor:
    fullname: V Cerantola
– volume: 277
  start-page: 25512
  year: 2002
  ident: BFsrep16319_CR20
  publication-title: J Biol Chem
  doi: 10.1074/jbc.M202947200
  contributor:
    fullname: P Ternes
– volume: 263
  start-page: 1629
  year: 1994
  ident: BFsrep16319_CR40
  publication-title: Science
  doi: 10.1126/science.8128252
  contributor:
    fullname: P Cosson
– volume: 4
  start-page: 662
  year: 2005
  ident: BFsrep16319_CR38
  publication-title: Mol Cell Proteomics
  doi: 10.1074/mcp.M400123-MCP200
  contributor:
    fullname: K Natter
– volume: 286
  start-page: 6769
  year: 2011
  ident: BFsrep16319_CR44
  publication-title: J Biol Chem
  doi: 10.1074/jbc.M110.176875
  contributor:
    fullname: C Vionnet
– volume: 86
  start-page: 1246
  year: 2012
  ident: BFsrep16319_CR30
  publication-title: Mol Microbiol
  doi: 10.1111/mmi.12056
  contributor:
    fullname: K Kajiwara
– volume: 24
  start-page: 841
  year: 2006
  ident: BFsrep16319_CR37
  publication-title: Nat Biotechnol
  doi: 10.1038/nbt1222
  contributor:
    fullname: A Matsuyama
– volume: 71
  start-page: 177
  year: 2014
  ident: BFsrep16319_CR11
  publication-title: J Am Acad Dermatol
  doi: 10.1016/j.jaad.2014.01.891
  contributor:
    fullname: MH Meckfessel
– volume: 96
  start-page: 523
  year: 1991
  ident: BFsrep16319_CR5
  publication-title: J Invest Dermatol
  doi: 10.1111/1523-1747.ep12470233
  contributor:
    fullname: G Imokawa
– volume: 1841
  start-page: 657
  year: 2014
  ident: BFsrep16319_CR42
  publication-title: Biochim Biophys Acta
  doi: 10.1016/j.bbalip.2013.08.006
  contributor:
    fullname: X Huang
– volume: 1758
  start-page: 2080
  year: 2006
  ident: BFsrep16319_CR1
  publication-title: Biochim Biophys Acta
  doi: 10.1016/j.bbamem.2006.06.021
  contributor:
    fullname: JA Bouwstra
– volume: 20
  start-page: 356
  year: 2010
  ident: BFsrep16319_CR16
  publication-title: J Microbiol Biotechnol
  doi: 10.4014/jmb.0909.09041
  contributor:
    fullname: SK Kim
– volume: 36
  start-page: 6960
  year: 1997
  ident: BFsrep16319_CR36
  publication-title: Biochemistry
  doi: 10.1021/bi970091l
  contributor:
    fullname: DL Cadena
– volume: 49
  start-page: 909
  year: 2008
  ident: BFsrep16319_CR23
  publication-title: J Lipid Res
  doi: 10.1194/jlr.R800003-JLR200
  contributor:
    fullname: RC Dickson
– volume: 9
  start-page: 3153
  year: 1990
  ident: BFsrep16319_CR39
  publication-title: Embo j
  doi: 10.1002/j.1460-2075.1990.tb07513.x
  contributor:
    fullname: MR Jackson
– volume: 272
  start-page: 29704
  year: 1997
  ident: BFsrep16319_CR22
  publication-title: J Biol Chem
  doi: 10.1074/jbc.272.47.29704
  contributor:
    fullname: D Haak
– volume: 196
  start-page: 1086
  year: 2012
  ident: BFsrep16319_CR19
  publication-title: New Phytol
  doi: 10.1111/j.1469-8137.2012.04351.x
  contributor:
    fullname: S Konig
– volume: 563
  start-page: 93
  year: 2004
  ident: BFsrep16319_CR34
  publication-title: FEBS Lett
  doi: 10.1016/S0014-5793(04)00274-1
  contributor:
    fullname: Y Mizutani
– volume: 19
  start-page: 2069
  year: 2008
  ident: BFsrep16319_CR45
  publication-title: Mol Biol Cell
  doi: 10.1091/mbc.e07-08-0740
  contributor:
    fullname: K Kajiwara
– volume: 91
  start-page: 784
  year: 2009
  ident: BFsrep16319_CR3
  publication-title: Biochimie
  doi: 10.1016/j.biochi.2009.04.001
  contributor:
    fullname: Y Mizutani
– volume: 66
  start-page: 1671
  year: 2004
  ident: BFsrep16319_CR47
  publication-title: Mol Pharmacol
  doi: 10.1124/mol.104.003681
  contributor:
    fullname: G Triola
SSID ssj0000529419
Score 2.3217773
Snippet Ceramide is one of the most important intercellular components responsible for the barrier and moisture retention functions of the skin. Because of the risks...
Abstract Ceramide is one of the most important intercellular components responsible for the barrier and moisture retention functions of the skin. Because of...
SourceID pubmedcentral
proquest
crossref
pubmed
springer
SourceType Open Access Repository
Aggregation Database
Index Database
Publisher
StartPage 16319
SubjectTerms 101/58
45/29
631/326/2522
631/61/318
82/16
Amidohydrolases - genetics
Amidohydrolases - metabolism
Amino Acid Sequence
Antifungal Agents - pharmacology
C gene
Ceramidase
Ceramide
Ceramides - analysis
Ceramides - metabolism
Chromatography, High Pressure Liquid
Depsipeptides - pharmacology
Desaturase
Endoplasmic reticulum
Endoplasmic Reticulum - metabolism
Humanities and Social Sciences
Humans
Industrial Microbiology - methods
Inositol
Localization
Metabolic Engineering
Microscopy, Fluorescence
Mixed Function Oxygenases - deficiency
Mixed Function Oxygenases - genetics
multidisciplinary
Oxidoreductases - genetics
Oxidoreductases - metabolism
Phospholipase C
Saccharomyces cerevisiae - drug effects
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins - genetics
Saccharomyces cerevisiae Proteins - metabolism
Science
Skin
Sphingolipids
Sphingosine - analysis
Sphingosine - metabolism
Tandem Mass Spectrometry
Type C Phospholipases - genetics
Type C Phospholipases - metabolism
Yeast
SummonAdditionalLinks – databaseName: Health & Medical Collection
  dbid: 7X7
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV3dSxwxEA9WKfSlVO3Htipp9TW4u9lkkqdSSkV8kIIVzqcln-1Bb--q58P9983kdtcv8DkJGTKT5JfMzG8IOQrcpH3gPRNeRdYYXTMdrWaei4j0YqBjjrY4l6eXzdlETPoPt5s-rHI4E_NB7ecO_8iPK3wYVAACvi7-Mawahd7VvoTGC7JV1cl4kz3DBMY_FvRiNZUeCIW4Ok7XziIhEOTVuX8NPcGWT0MkH_lJ8_Vz8oa87nEj_bZW9DbZCN0OebmuJLnaJVc_M3NrGkpz1T3qwrWZTX1g5xfUrugsLJO2_04dDXcEhBSD3n_TFZbvoRfGYQbWfLZKJwcOz2nnJrwllyc_fn0_ZX3VBOZE2SyZiUJbwU0To40J7jjAMFFvaqnBeB24sLxWvhQOoZQrrVLaKSuikpAAkObvyGY378IHzOeWJgLnFmrfQNDGcakr76OKEiyUBfkyLGK7WJNjtNmpzVU7rnRB9oblbfv9cdPeabMgn8fmZNnorjBdmN-mPsCxpGl68hTk_Vob4yy1FMAbmSSAB3oaOyBr9sOWbvons2c3SJhfyoIcDhq9J9Zj4T8-L_wn8ioBKIG5iRXskc3l9W3YTyBlaQ-yJf4H7Ozqdw
  priority: 102
  providerName: ProQuest
– databaseName: Scholars Portal Open Access Journals
  dbid: M48
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwjV1LS8QwEA6iCF7Et_VFfFyrbdO8DiIiingQQRf0VPLUBber6wr235tpt6urHjwnocNM0vlCZr4PoQNHVDgH1sbUCh_nSmax9FrGllAP9GJc-rra4ppddvKre3o_hVqNzZED3_682oGeVGfwfPjxWp2EA3_ctIyLo5BLXgKsAPLPmQz4uKCCb4TyG4rvTOapbHmFvq-YzEa_IObvSskfz6V1FrpYQPMj-IhPm3gvoilXLqHZRlCyWkYPNzWBa1iKa_E9bNxA9brWxde3WFe454Yh6M9dg90XDyGG2vdHXIGKD75VBhqx-r0q_EBged19rtwK6lyc351dxiPxhNjQJB_GylOpKVG599oH1GM4VItalTHJlZWOUE0yYRNqAFGZRAshjdDUC8YDDpJkFU2X_dKtQ1s3U54Tonlmc-6kMoTJ1FovPOOaJxHaa51YvDQcGUX9tk1EMfZ0hLZa9xZtlIsUbnsp55RHaHc8HDY4vFqo0vXfwxxOQNk03HwitNZEY_yVjFFOchYs4BNxGk8A8uzJkbL7VJNo58Cbn7AI7bcR_WbWT-M3_mHdJpoLYIpCn2LKt9D0cPDutgNgGeqdejt-AlHh7no
  priority: 102
  providerName: Scholars Portal
– databaseName: SpringerOpen
  dbid: C6C
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwjV1LS8NAEF5EEbyIb6NVtuo1mGSzr6MUpXgoggp6CvvUgk2L1kP-vTtpE1t78byz7LCzm_k2M_MNQleOqHAPrI2pFT7Olcxi6bWMLaEe6MW49HW2xYD1n_P7F_qyhrpNLcxS_J6I6-AnJgEyALHnBrhe6M7QY732NwoEqvJUNpxBizOWPc0KfFzNgvwTCq09zN0O2p5DQ3wzs-UuWnPlHtqcNYus9tHrQ03OGqbiurEeNu5TjYbWxYNHrCs8ctNg0I-hwe6XYxBDXvsbrqBDD35UBoqsxqMqfBxgel1ZrtwBer67fer143ljhNjQJJ_GylOpKVG599oHRGM4ZIJalTHJlZWOUE0yYRNqAC2ZRAshjdDUC8YDxpHkEK2X49IdQ8k2U54Tonlmc-6kMoTJ1FovPOOaJxG6aDaxmMz4L4o6bk1E0e50hDrN9hbzK_BVpPCSSzmnPELddjgcXohIqNKNv4MMJ9C1NLxqInQ0s0a7SsYoJzkLGvAlO7UCQIy9PFIO32uC7Bw48RMWocvGogtq_VX-5F9Sp2grQCUKVYgp76D16ee3OwtwZKrP6wP5A2IP4HM
  priority: 102
  providerName: Springer Nature
Title Producing human ceramide-NS by metabolic engineering using yeast Saccharomyces cerevisiae
URI https://link.springer.com/article/10.1038/srep16319
https://www.ncbi.nlm.nih.gov/pubmed/26573460
https://www.proquest.com/docview/1899817757
https://search.proquest.com/docview/1735900559
https://pubmed.ncbi.nlm.nih.gov/PMC4647206
Volume 5
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1bT9swFD4Cpkl7QbsvjFXe5TU0ie-PUIHQJKpqDKl7inxllUhaQXnov8d2mq7A214iRbYTy-c4_hx_5zsAPxxWYR5Ym1MrfE6UrHLptcwtpj7Ki3HpE9tizM6vyM8pne4A7WNhEmnf6NlRe9MctbO_iVu5aMyw54kNJxcjEjXPCzbchd3goFtb9E7Qu5KklL2KEBbDsNYsAuwoo0JoxSjHJAlSbi1Dz7Dlc4rkk3PStPycvYb9NW5Ex13_3sCOa9_Cyy6T5Ood_Jkk5dbQFKWse8i4W9XMrMvHl0ivUOOWwdo3M4PcPwFCFEnv12gV0_egS2ViBNa8WYUvR2yews6Vew9XZ6e_R-f5OmtCbmhBlrnyVGqKFfFe-wB3DI80UasqJrmy0mGqcSVsQU2EUqbQQkgjNPWC8QCAJP4Ae-28dZ9iPDdTnmOseWUJd1IZzGRprReecc2LDL71g1gvOnGMOh1qY1FvBj2Dw3546_X8uKvLuM0rOac8g6-b4uDZ8bhCtW5-H-pwHFOahi1PBh87a2ze0psxA_7ITpsKUTX7cUlwpqSevXaeDL73Ft3q1tPOH_z38z_Dq4CtaAxbLPkh7C1v792XgF-WehC8dsoH8OLkdDz5Fe5GbDRI_wLC9YKIQfLnBzD4-HM
link.rule.ids 230,315,730,783,787,867,888,12070,21402,24332,27938,27939,31733,31734,33758,33759,41134,42203,43324,43819,51590,53806,53808,74081,74638
linkProvider National Library of Medicine
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1Lb9QwELagCMEF8SZQwDyuVpM49tgnhBDVAmWF1FZaTpGfsBKbXdrtYf89Hm-SviTOdpSRx4_PnpnvI-R94CatA--Z8Cqyxuia6Wg181xEpBcDHXO2xVROjpuvMzHrH9xO-7TKYU_MG7VfOnwj36vwYlABCPiw-stQNQqjq72Exk1yC3m4kDsfZjC-sWAUq6n0QCjE1V46dlYJgSCvzsVj6Bq2vJ4ieSVOmo-f_fvkXo8b6cetox-QG6F7SG5vlSQ3j8jPH5m5NX1Ks-oedeHELOY-sOkhtRu6COvk7T9zR8M5ASHFpPdfdIPyPfTQOKzAWi42aefAz3PZuQmPyfH-56NPE9arJjAnymbNTBTaCm6aGG1McMcBpol6U0sNxuvAheW18qVwCKVcaZXSTlkRlYQEgDR_Qna6ZReeYT23NBE4t1D7BoI2jktdeR9VlGChLMjbYRDb1ZYco81Bba7acaQLsjsMb9uvj9P23JsFeTM2p5mN4QrTheVZ6gMcJU3TlacgT7feGP9SSwG8kckCuOSnsQOyZl9u6ea_M3t2g4T5pSzIu8GjF8y6avzz_xv_mtyZHH0_aA--TL-9IHcTmBJYp1jBLtlZn5yFlwmwrO2rPCv_ARy07Wc
linkToPdf http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1Lb9QwELagCMQF8SZQwDyuVpM49tgnhIBVeWhVqVRaTpGfZSU2u7Tbw_57PN4kfUmc7Sgjz9jz2TPzDSHvAzdpH3jPhFeRNUbXTEermeciIr0Y6JizLaZy_6j5NhOzPv_ptE-rHM7EfFD7pcM38r0KLwYVQLrAxz4t4uDz5MPqL8MOUhhp7dtp3CS3oEmOLtk2zGB8b8GIVlPpgVyIq73kglYJjSDHzkWXdA1nXk-XvBIzza5ocp_c6zEk_bhV-gNyI3QPye1tV8nNI_LrILO4pk9p7sBHXTgxi7kPbHpI7YYuwjpp_s_c0XBORkgxAf6YbrCVDz00DquxlotNOkXw81yCbsJjcjT58vPTPus7KDAnymbNTBTaCm6aGG1M0McBpox6U0sNxuvAheW18qVwCKtcaZXSTlkRlYQEhjR_Qna6ZReeYW23NBE4t1D7BoI2jktdeR9VlGChLMjbYRHb1ZYoo80Bbq7acaULsjssb9vvldP2XLMFeTMOJyvH0IXpwvIszQGO7U3T9acgT7faGP9SSwG8kUkCuKSncQIyaF8e6ea_M5N2g-T5pSzIu0GjF8S6Kvzz_wv_mtxJBtn--Dr9_oLcTbhKYMliBbtkZ31yFl4m7LK2r7JR_gP15_Gc
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Producing+human+ceramide-NS+by+metabolic+engineering+using+yeast+Saccharomyces+cerevisiae&rft.jtitle=Scientific+reports&rft.au=Murakami%2C+Suguru&rft.au=Shimamoto%2C+Toshi&rft.au=Nagano%2C+Hideaki&rft.au=Tsuruno%2C+Masahiro&rft.date=2015-11-17&rft.eissn=2045-2322&rft.volume=5&rft.spage=16319&rft.epage=16319&rft_id=info:doi/10.1038%2Fsrep16319&rft.externalDBID=NO_FULL_TEXT
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2045-2322&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2045-2322&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2045-2322&client=summon