High-throughput cryopreservation of plant cell cultures for functional genomics

• Published in: Plant and cell physiology Vol. 53; no. 5; pp. 943 - 952
• Main Authors: Ogawa, Yoichi, Sakurai, Nozomu, Oikawa, Akira, Kai, Kosuke, Morishita, Yoshihiko, Mori, Kumiko, Moriya, Kanami, Fujii, Fumiko, Aoki, Koh, Suzuki, Hideyuki, Ohta, Daisaku, Saito, Kazuki, Shibata, Daisuke
• Format: Journal Article
• Language: English
• Published: Japan Oxford University Press 01.05.2012
• Subjects: Arabidopsis - cytology; Arabidopsis - genetics; Arabidopsis - physiology; Arabidopsis thaliana; Cell culture; Cell Culture Techniques - methods; Cell Line; Cell Survival - drug effects; Cryopreservation; Cryopreservation - methods; Cryoprotective Agents - pharmacology; Daucus carota; Freezing; Gene expression; Gene Expression Regulation, Plant - drug effects; Genetic engineering; genomics; Genomics - methods; Glucuronidase - metabolism; Glycerol; High-Throughput Screening Assays - methods; Lotus japonicus; Metabolism; Nicotiana tabacum; Nitrogen; Oryza sativa; Plant cells; Plant Cells - drug effects; Plant Cells - metabolism; polystyrene; Proline; Proline - pharmacology; Sucrose; Techniques; Temperature effects

Suspension-cultured cell lines from plant species are useful for genetic engineering. However, maintenance of these lines is laborious, involves routine subculturing and hampers wider use of transgenic lines, especially when many lines are required for a high-throughput functional genomics applicati...