Stem cell differentiation increases membrane-actin adhesion regulating cell blebability, migration and mechanics

• Published in: Scientific reports Vol. 4; no. 1; p. 7307
• Main Authors: Sliogeryte, Kristina, Thorpe, Stephen D., Lee, David A., Botto, Lorenzo, Knight, Martin M.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 04.12.2014; Nature Publishing Group
• Subjects: 13; 14; 14/19; 38; 38/22; 631/532/2074; 631/57; 82; 82/1; Actin; Actins - metabolism; Cell adhesion & migration; Cell Differentiation - physiology; Cell Membrane - metabolism; Cell Membrane - physiology; Cell migration; Cell Movement - physiology; Cells, Cultured; Cytoskeletal Proteins - metabolism; Ezrin; Humanities and Social Sciences; Humans; Membrane Proteins - metabolism; Mesenchymal Stromal Cells - metabolism; Mesenchymal Stromal Cells - physiology; Mesenchyme; multidisciplinary; Radixin; Science; Stem cells; Transfection

This study examines how differentiation of human mesenchymal stem cells regulates the interaction between the cell membrane and the actin cortex controlling cell behavior. Micropipette aspiration was used to measure the pressure required for membrane-cortex detachment which increased from 0.15 kPa i...