Next-Generation Sequencing: From Basic Research to Diagnostics

Background: For the past 30 years, the Sanger method has been the dominant approach and gold standard for DNA sequencing. The commercial launch of the first massively parallel pyrosequencing platform in 2005 ushered in the new era of high-throughput genomic analysis now referred to as next-generatio...

Full description

Saved in:

Bibliographic Details
Published in	Clinical chemistry (Baltimore, Md.) Vol. 55; no. 4; pp. 641 - 658
Main Authors	Voelkerding, Karl V, Dames, Shale A, Durtschi, Jacob D
Format	Journal Article
Language	English
Published	Washington, DC Am Assoc Clin Chem 01.04.2009 American Association for Clinical Chemistry Oxford University Press
Subjects	Accuracy Analytical, structural and metabolic biochemistry Animals Base Sequence - genetics Bioinformatics Biological and medical sciences Biomedical Research - methods Data processing Deoxyribonucleic acid DNA Fundamental and applied biological sciences. Psychology Genomics Human subjects Humans Industrial development Investigative techniques, diagnostic techniques (general aspects) Life sciences Medical sciences Molecular Diagnostic Techniques - methods Peer Review, Research - methods Software Technological change Validation studies Nucleotide sequence Clinical biology Biochemistry Diagnosis Fundamental research Molecular biology Sequencing
Online Access	Get full text

Cover

Loading…

Abstract	Background: For the past 30 years, the Sanger method has been the dominant approach and gold standard for DNA sequencing. The commercial launch of the first massively parallel pyrosequencing platform in 2005 ushered in the new era of high-throughput genomic analysis now referred to as next-generation sequencing (NGS). Content: This review describes fundamental principles of commercially available NGS platforms. Although the platforms differ in their engineering configurations and sequencing chemistries, they share a technical paradigm in that sequencing of spatially separated, clonally amplified DNA templates or single DNA molecules is performed in a flow cell in a massively parallel manner. Through iterative cycles of polymerase-mediated nucleotide extensions or, in one approach, through successive oligonucleotide ligations, sequence outputs in the range of hundreds of megabases to gigabases are now obtained routinely. Highlighted in this review are the impact of NGS on basic research, bioinformatics considerations, and translation of this technology into clinical diagnostics. Also presented is a view into future technologies, including real-time single-molecule DNA sequencing and nanopore-based sequencing. Summary: In the relatively short time frame since 2005, NGS has fundamentally altered genomics research and allowed investigators to conduct experiments that were previously not technically feasible or affordable. The various technologies that constitute this new paradigm continue to evolve, and further improvements in technology robustness and process streamlining will pave the path for translation into clinical diagnostics.
AbstractList	For the past 30 years, the Sanger method has been the dominant approach and gold standard for DNA sequencing. The commercial launch of the first massively parallel pyrosequencing platform in 2005 ushered in the new era of high-throughput genomic analysis now referred to as next-generation sequencing (NGS).BACKGROUNDFor the past 30 years, the Sanger method has been the dominant approach and gold standard for DNA sequencing. The commercial launch of the first massively parallel pyrosequencing platform in 2005 ushered in the new era of high-throughput genomic analysis now referred to as next-generation sequencing (NGS).This review describes fundamental principles of commercially available NGS platforms. Although the platforms differ in their engineering configurations and sequencing chemistries, they share a technical paradigm in that sequencing of spatially separated, clonally amplified DNA templates or single DNA molecules is performed in a flow cell in a massively parallel manner. Through iterative cycles of polymerase-mediated nucleotide extensions or, in one approach, through successive oligonucleotide ligations, sequence outputs in the range of hundreds of megabases to gigabases are now obtained routinely. Highlighted in this review are the impact of NGS on basic research, bioinformatics considerations, and translation of this technology into clinical diagnostics. Also presented is a view into future technologies, including real-time single-molecule DNA sequencing and nanopore-based sequencing.CONTENTThis review describes fundamental principles of commercially available NGS platforms. Although the platforms differ in their engineering configurations and sequencing chemistries, they share a technical paradigm in that sequencing of spatially separated, clonally amplified DNA templates or single DNA molecules is performed in a flow cell in a massively parallel manner. Through iterative cycles of polymerase-mediated nucleotide extensions or, in one approach, through successive oligonucleotide ligations, sequence outputs in the range of hundreds of megabases to gigabases are now obtained routinely. Highlighted in this review are the impact of NGS on basic research, bioinformatics considerations, and translation of this technology into clinical diagnostics. Also presented is a view into future technologies, including real-time single-molecule DNA sequencing and nanopore-based sequencing.In the relatively short time frame since 2005, NGS has fundamentally altered genomics research and allowed investigators to conduct experiments that were previously not technically feasible or affordable. The various technologies that constitute this new paradigm continue to evolve, and further improvements in technology robustness and process streamlining will pave the path for translation into clinical diagnostics.SUMMARYIn the relatively short time frame since 2005, NGS has fundamentally altered genomics research and allowed investigators to conduct experiments that were previously not technically feasible or affordable. The various technologies that constitute this new paradigm continue to evolve, and further improvements in technology robustness and process streamlining will pave the path for translation into clinical diagnostics. For the past 30 years, the Sanger method has been the dominant approach and gold standard for DNA sequencing. The commercial launch of the first massively parallel pyrosequencing platform in 2005 ushered in the new era of high-throughput genomic analysis now referred to as next-generation sequencing (NGS). This review describes fundamental principles of commercially available NGS platforms. Although the platforms differ in their engineering configurations and sequencing chemistries, they share a technical paradigm in that sequencing of spatially separated, clonally amplified DNA templates or single DNA molecules is performed in a flow cell in a massively parallel manner. Through iterative cycles of polymerase-mediated nucleotide extensions or, in one approach, through successive oligonucleotide ligations, sequence outputs in the range of hundreds of megabases to gigabases are now obtained routinely. Highlighted in this review are the impact of NGS on basic research, bioinformatics considerations, and translation of this technology into clinical diagnostics. Also presented is a view into future technologies, including real-time single-molecule DNA sequencing and nanopore-based sequencing. In the relatively short time frame since 2005, NGS has fundamentally altered genomics research and allowed investigators to conduct experiments that were previously not technically feasible or affordable. The various technologies that constitute this new paradigm continue to evolve, and further improvements in technology robustness and process streamlining will pave the path for translation into clinical diagnostics. For the past 30 years, the Sanger method has been the dominant approach and gold standard for DNA sequencing. The commercial launch of the first massively parallel pyrosequencing platform in 2005 ushered in the new era of high-throughput genomic analysis now referred to as next-generation sequencing (NGS). This review describes fundamental principles of commercially available NGS platforms. Although the platforms differ in their engineering configurations and sequencing chemistries, they share a technical paradigm in that sequencing of spatially separated, clonally amplified DNA templates or single DNA molecules is performed in a flow cell in a massively parallel manner. Through iterative cycles of polymerase-mediated nucleotide extensions or, in one approach, through successive oligonucleotide ligations, sequence outputs in the range of hundreds of megabases to gigabases are now obtained routinely. Highlighted in this review are the impact of NGS on basic research, bioinformatics considerations, and translation of this technology into clinical diagnostics. Also presented is a view into future technologies, including real-time single-molecule DNA sequencing and nanopore-based sequencing. In the relatively short time frame since 2005, NGS has fundamentally altered genomics research and allowed investigators to conduct experiments that were previously not technically feasible or affordable. The various technologies that constitute this new paradigm continue to evolve, and further improvements in technology robustness and process streamlining will pave the path for translation into clinical diagnostics. Background: For the past 30 years, the Sanger method has been the dominant approach and gold standard for DNA sequencing. The commercial launch of the first massively parallel pyrosequencing platform in 2005 ushered in the new era of high-throughput genomic analysis now referred to as next-generation sequencing (NGS). Content: This review describes fundamental principles of commercially available NGS platforms. Although the platforms differ in their engineering configurations and sequencing chemistries, they share a technical paradigm in that sequencing of spatially separated, clonally amplified DNA templates or single DNA molecules is performed in a flow cell in a massively parallel manner. Through iterative cycles of polymerase-mediated nucleotide extensions or, in one approach, through successive oligonucleotide ligations, sequence outputs in the range of hundreds of megabases to gigabases are now obtained routinely. Highlighted in this review are the impact of NGS on basic research, bioinformatics considerations, and translation of this technology into clinical diagnostics. Also presented is a view into future technologies, including real-time single-molecule DNA sequencing and nanopore-based sequencing. Summary: In the relatively short time frame since 2005, NGS has fundamentally altered genomics research and allowed investigators to conduct experiments that were previously not technically feasible or affordable. The various technologies that constitute this new paradigm continue to evolve, and further improvements in technology robustness and process streamlining will pave the path for translation into clinical diagnostics.
Author	Durtschi, Jacob D Dames, Shale A Voelkerding, Karl V
Author_xml	– sequence: 1 fullname: Voelkerding, Karl V – sequence: 2 fullname: Dames, Shale A – sequence: 3 fullname: Durtschi, Jacob D
BackLink	http://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21325635$$DView record in Pascal Francis https://www.ncbi.nlm.nih.gov/pubmed/19246620$$D View this record in MEDLINE/PubMed
BookMark	eNqFkd1rFDEUxYNU7Hb1PxAZBH2bNXcyySR9ELTaKhQFP55DPu7spsxkajLL6n9v1t0q9MWHEAK_c3LvOWfkJE4RCXkKdAWsY6_cEKLb4LhqKJUrgKaT6gFZAGe0llzACVlQSlWtoO1OyVnON-XZdlI8IqegmlaIhi7I60_4c66vMGIyc5hi9RV_bDG6ENfn1WWaxuqtycFVXzCjSW5TzVP1Lph1nPIcXH5MHvZmyPjkeC_J98v33y4-1Nefrz5evLmuHaftXCvfKwO9RS8o6xrqe2Ed7bn03lOknUAvQUpjlLLCKgvcGATwDKy0nW3Ykrw8-N6mqcyXZz2G7HAYTMRpm7XoqAQueQGf3wNvpm2KZTbdAFOS8XKW5NkR2toRvb5NYTTpl76LpQAvjoDJzgx9MiWR_JcrTg0XbP9be-BcmnJO2P-zonrfkr5rSe9b0oeWiuz8nsyF-U_8czJh-J_4ONsmrDe7kFDn0QxDWQX0brfjXLdatMB-A6DKpkI
CODEN	CLCHAU
CitedBy_id	crossref_primary_10_1093_plphys_kiad273 crossref_primary_10_4018_IJKDB_2017070105 crossref_primary_10_1016_j_ekir_2024_12_020 crossref_primary_10_1111_cla_12012 crossref_primary_10_1007_s12223_012_0179_5 crossref_primary_10_1200_JCO_2009_27_1361 crossref_primary_10_1373_clinchem_2013_209213 crossref_primary_10_1007_s00122_019_03306_3 crossref_primary_10_1038_s41598_018_33051_4 crossref_primary_10_1111_j_1600_0757_2009_00316_x crossref_primary_10_1186_s13073_014_0071_9 crossref_primary_10_1186_s12859_016_1097_3 crossref_primary_10_3389_fgene_2022_1114762 crossref_primary_10_1093_jac_dku050 crossref_primary_10_3390_life12101583 crossref_primary_10_1093_cvr_cvr126 crossref_primary_10_1016_j_jmoldx_2012_01_013 crossref_primary_10_1517_17530059_2012_703650 crossref_primary_10_1021_acs_analchem_0c01202 crossref_primary_10_1093_annonc_mdu145 crossref_primary_10_3390_jcm8101646 crossref_primary_10_1109_ACCESS_2019_2938058 crossref_primary_10_1371_journal_pgen_1002496 crossref_primary_10_1038_ejhg_2011_138 crossref_primary_10_1016_j_tig_2009_05_004 crossref_primary_10_1002_pd_5038 crossref_primary_10_1016_j_trac_2015_03_008 crossref_primary_10_1039_C9AN02263E crossref_primary_10_1128_mSphere_00695_19 crossref_primary_10_3390_v3101849 crossref_primary_10_1002_humu_21327 crossref_primary_10_1016_j_mgene_2019_100586 crossref_primary_10_17660_ActaHortic_2024_1401_36 crossref_primary_10_1371_journal_pcbi_1003345 crossref_primary_10_1038_ejhg_2011_223 crossref_primary_10_2217_fon_09_113 crossref_primary_10_1016_j_ctrv_2013_05_001 crossref_primary_10_1097_QCO_0000000000000567 crossref_primary_10_1111_j_1751_553X_2011_01364_x crossref_primary_10_2217_fvl_13_21 crossref_primary_10_1155_2014_542541 crossref_primary_10_1088_1757_899X_490_4_042015 crossref_primary_10_1111_j_1600_0757_2010_00380_x crossref_primary_10_1021_acs_jpclett_3c00635 crossref_primary_10_1186_s12907_015_0020_6 crossref_primary_10_1080_07853890_2017_1392595 crossref_primary_10_1371_journal_pone_0155683 crossref_primary_10_1038_ejhg_2012_270 crossref_primary_10_1371_journal_pone_0102826 crossref_primary_10_1373_clinchem_2009_141267 crossref_primary_10_1007_s13258_015_0361_7 crossref_primary_10_1002_humu_22439 crossref_primary_10_1007_s00705_023_05747_0 crossref_primary_10_3390_ijms18040805 crossref_primary_10_1016_j_copbio_2010_02_009 crossref_primary_10_1016_j_jcoa_2024_100121 crossref_primary_10_7314_APJCP_2015_16_5_1693 crossref_primary_10_1111_1462_2920_15097 crossref_primary_10_2527_jas_2015_9225 crossref_primary_10_18632_oncotarget_8076 crossref_primary_10_1016_j_virusres_2015_11_001 crossref_primary_10_1186_1471_2164_12_116 crossref_primary_10_1016_j_jmoldx_2012_02_002 crossref_primary_10_1186_1471_2164_12_598 crossref_primary_10_1371_journal_pone_0188181 crossref_primary_10_1007_s13206_012_6407_x crossref_primary_10_3390_jpm10020050 crossref_primary_10_1038_s41598_021_85803_4 crossref_primary_10_1016_j_critrevonc_2015_06_007 crossref_primary_10_4161_viru_26652 crossref_primary_10_1016_j_ymeth_2020_10_002 crossref_primary_10_1128_JCM_00370_10 crossref_primary_10_1186_s13148_022_01323_6 crossref_primary_10_1007_s12282_018_0924_y crossref_primary_10_1371_journal_pone_0256126 crossref_primary_10_1007_s00335_019_09816_1 crossref_primary_10_1016_j_fsigen_2015_02_002 crossref_primary_10_1016_j_bbrc_2015_10_110 crossref_primary_10_1039_C4TC00460D crossref_primary_10_1038_s41592_023_01914_y crossref_primary_10_1007_s11033_019_05137_3 crossref_primary_10_1016_j_scitotenv_2018_04_379 crossref_primary_10_1186_s13073_017_0441_1 crossref_primary_10_1007_s15005_022_2446_6 crossref_primary_10_3390_ijms241813802 crossref_primary_10_1089_cmb_2010_0127 crossref_primary_10_3390_pathogens12081061 crossref_primary_10_1186_1471_2156_14_6 crossref_primary_10_1016_j_drudis_2011_03_006 crossref_primary_10_1002_em_20630 crossref_primary_10_1093_molbev_msp289 crossref_primary_10_1007_s11886_014_0507_2 crossref_primary_10_1111_1471_0307_12789 crossref_primary_10_1200_EDBK_175397 crossref_primary_10_1088_0957_4484_27_21_215502 crossref_primary_10_1177_1740774513497541 crossref_primary_10_3390_diagnostics11071195 crossref_primary_10_1007_s00414_020_02327_8 crossref_primary_10_3389_fpls_2014_00216 crossref_primary_10_1016_j_theriogenology_2011_11_009 crossref_primary_10_1007_s00129_016_3883_4 crossref_primary_10_2353_jmoldx_2010_100043 crossref_primary_10_1016_j_cbpa_2015_05_020 crossref_primary_10_1111_j_1423_0410_2010_01444_x crossref_primary_10_1093_bioinformatics_bty773 crossref_primary_10_3390_plants12234006 crossref_primary_10_1038_jhg_2013_86 crossref_primary_10_3389_fgene_2020_544162 crossref_primary_10_1038_s41598_019_48467_9 crossref_primary_10_1371_journal_pone_0012295 crossref_primary_10_4137_CIN_S22941 crossref_primary_10_1093_cdn_nzz082 crossref_primary_10_1002_elps_201100344 crossref_primary_10_1007_s11568_011_9156_3 crossref_primary_10_1038_s41598_019_55282_9 crossref_primary_10_21320_2500_2139_2022_15_4_307_326 crossref_primary_10_1007_s10725_018_0462_9 crossref_primary_10_3390_ijms20102530 crossref_primary_10_1186_s12929_015_0138_y crossref_primary_10_1016_j_plefa_2009_04_001 crossref_primary_10_1109_TC_2020_3041402 crossref_primary_10_1128_CMR_00003_14 crossref_primary_10_1016_j_ymeth_2015_04_030 crossref_primary_10_1186_1471_2164_15_405 crossref_primary_10_1016_j_disamonth_2020_101034 crossref_primary_10_3390_biomedicines12030682 crossref_primary_10_1016_j_yexmp_2016_07_010 crossref_primary_10_3389_fonc_2020_01215 crossref_primary_10_1089_omi_2015_0191 crossref_primary_10_1039_B900409M crossref_primary_10_1016_j_clp_2012_12_005 crossref_primary_10_1371_journal_pone_0099033 crossref_primary_10_1186_1471_2105_13_S14_S9 crossref_primary_10_3109_01677063_2015_1060972 crossref_primary_10_1080_03772063_2019_1603085 crossref_primary_10_1186_1471_2105_11_269 crossref_primary_10_1016_j_jgg_2011_02_003 crossref_primary_10_1016_j_transci_2011_06_002 crossref_primary_10_1016_j_ygeno_2015_11_003 crossref_primary_10_1371_journal_pone_0163595 crossref_primary_10_1002_jcp_22009 crossref_primary_10_1111_mmi_12118 crossref_primary_10_1038_srep00261 crossref_primary_10_1038_cr_2012_30 crossref_primary_10_1126_scisignal_287re6 crossref_primary_10_1016_j_gene_2012_06_020 crossref_primary_10_1155_2012_741542 crossref_primary_10_1371_journal_pone_0201813 crossref_primary_10_1080_08820538_2020_1776343 crossref_primary_10_1016_j_cancergen_2013_10_003 crossref_primary_10_3390_life12111893 crossref_primary_10_1007_s13762_015_0793_2 crossref_primary_10_1371_journal_pone_0037919 crossref_primary_10_20517_mrr_2024_01 crossref_primary_10_1039_D0CS00936A crossref_primary_10_1128_JCM_00850_11 crossref_primary_10_3389_fmed_2022_824122 crossref_primary_10_1002_path_2645 crossref_primary_10_1016_j_mrrev_2020_108341 crossref_primary_10_1007_s00335_014_9503_5 crossref_primary_10_1007_s11033_023_08844_0 crossref_primary_10_1080_00450618_2020_1807050 crossref_primary_10_1172_JCI39085 crossref_primary_10_1007_s11427_013_4439_7 crossref_primary_10_3390_genes1010085 crossref_primary_10_1371_journal_pone_0033213 crossref_primary_10_1016_j_ymgme_2010_03_004 crossref_primary_10_1039_C9NP00071B crossref_primary_10_2459_JCM_0000000000000589 crossref_primary_10_20960_revmedlab_00077 crossref_primary_10_1021_ac102666n crossref_primary_10_3390_ijms17081239 crossref_primary_10_33245_2310_9289_2023_182_2_90_99 crossref_primary_10_1186_gb_2011_12_2_217 crossref_primary_10_1186_1471_2105_12_5 crossref_primary_10_1007_s00335_015_9603_x crossref_primary_10_1371_journal_pone_0152774 crossref_primary_10_1007_s10115_023_02049_4 crossref_primary_10_1016_j_ijid_2021_11_029 crossref_primary_10_3923_jas_2013_4881_4886 crossref_primary_10_1186_1471_2105_14_176 crossref_primary_10_1016_j_jmoldx_2012_04_006 crossref_primary_10_1038_srep05907 crossref_primary_10_3389_fphys_2017_00612 crossref_primary_10_1111_j_1365_2591_2011_02006_x crossref_primary_10_4103_jpbs_jpbs_838_23 crossref_primary_10_1371_journal_pone_0116680 crossref_primary_10_1373_clinchem_2010_145441 crossref_primary_10_3390_toxins10070292 crossref_primary_10_1016_j_lungcan_2013_08_025 crossref_primary_10_1038_ejhg_2013_175 crossref_primary_10_1186_1471_2105_11_584 crossref_primary_10_1515_jlm_2010_054 crossref_primary_10_1007_s12263_012_0312_8 crossref_primary_10_3389_fcimb_2023_1179090 crossref_primary_10_1038_s41576_019_0113_7 crossref_primary_10_1097_PPO_0b013e31823bd1f8 crossref_primary_10_1186_1471_2105_15_S11_S6 crossref_primary_10_1586_erm_11_18 crossref_primary_10_1007_s10689_016_9955_8 crossref_primary_10_1016_j_biotechadv_2020_107537 crossref_primary_10_5321_wjs_v6_i2_6 crossref_primary_10_1016_j_paed_2009_11_004 crossref_primary_10_1089_omi_2017_0185 crossref_primary_10_1177_1010428317698376 crossref_primary_10_1038_nrg2544 crossref_primary_10_1016_j_rec_2015_10_001 crossref_primary_10_3402_jom_v4i0_10743 crossref_primary_10_7717_peerj_11724 crossref_primary_10_1007_s11047_021_09863_1 crossref_primary_10_1586_erm_11_23 crossref_primary_10_1007_s12525_023_00669_w crossref_primary_10_1016_j_snb_2017_10_140 crossref_primary_10_1586_epr_12_54 crossref_primary_10_1016_j_cca_2015_07_018 crossref_primary_10_3389_fcimb_2023_1140548 crossref_primary_10_1681_ASN_0000000000000401 crossref_primary_10_1016_j_yao_2021_02_009 crossref_primary_10_1089_gtmb_2012_0464 crossref_primary_10_1016_j_antinf_2017_10_003 crossref_primary_10_1016_j_bsheal_2020_06_001 crossref_primary_10_1016_j_ab_2014_07_014 crossref_primary_10_47570_joci_2024_002 crossref_primary_10_1017_S1751731110002120 crossref_primary_10_1373_jalm_2017_023119 crossref_primary_10_1371_journal_pone_0053083 crossref_primary_10_1016_j_pathog_2015_05_004 crossref_primary_10_1586_14737159_2016_1142374 crossref_primary_10_3389_fgene_2015_00119 crossref_primary_10_1136_jmedgenet_2015_103144 crossref_primary_10_1530_JME_13_0219 crossref_primary_10_1186_s12890_023_02337_3 crossref_primary_10_15407_oncology_2024_03_216 crossref_primary_10_1586_erv_09_67 crossref_primary_10_1155_2014_853076 crossref_primary_10_1002_jia2_25193 crossref_primary_10_1586_erm_11_3 crossref_primary_10_1016_j_recesp_2015_10_003 crossref_primary_10_1186_1471_2164_13_417 crossref_primary_10_1142_S1793524515300018 crossref_primary_10_1155_2012_132856 crossref_primary_10_1007_s13197_023_05889_8 crossref_primary_10_3109_09537104_2015_1109071 crossref_primary_10_1159_000480089 crossref_primary_10_1002_humu_22091 crossref_primary_10_1186_1471_2164_14_221 crossref_primary_10_1021_jp911407c crossref_primary_10_1309_AJCPBMXETHAPAV1E crossref_primary_10_14712_fb2009055060238 crossref_primary_10_2217_pme_13_41 crossref_primary_10_3389_fmicb_2024_1387623 crossref_primary_10_2217_pgs_16_21 crossref_primary_10_1186_1471_2164_13_500 crossref_primary_10_1080_14737159_2016_1209411 crossref_primary_10_1186_s12941_024_00714_2 crossref_primary_10_3343_alm_2013_33_3_159 crossref_primary_10_47115_bsagriculture_1296263 crossref_primary_10_1146_annurev_genom_083115_022545 crossref_primary_10_21597_jist_826047 crossref_primary_10_1038_ijo_2012_57 crossref_primary_10_1016_j_fsi_2020_03_005 crossref_primary_10_2217_bmm_11_37 crossref_primary_10_1016_j_brainresbull_2011_11_015 crossref_primary_10_2217_pme_11_76 crossref_primary_10_4161_15592324_2014_976152 crossref_primary_10_1007_s13258_018_0650_z crossref_primary_10_1186_s12886_024_03472_z crossref_primary_10_3390_biology10090916 crossref_primary_10_1309_AJCPR1SVT1VHUGXW crossref_primary_10_3390_jcm8081173 crossref_primary_10_1177_1177932219860817 crossref_primary_10_1159_000538176 crossref_primary_10_7717_peerj_6661 crossref_primary_10_2139_ssrn_2017413 crossref_primary_10_1371_journal_pone_0093662 crossref_primary_10_1002_biot_201000324 crossref_primary_10_1002_tcr_200900010 crossref_primary_10_5482_HAMO_17_01_0001 crossref_primary_10_1371_journal_pone_0048792 crossref_primary_10_2144_btn_2020_0057 crossref_primary_10_1093_jpids_piab037 crossref_primary_10_1111_crj_13538 crossref_primary_10_1016_j_fbr_2018_12_001 crossref_primary_10_1016_j_fsigen_2017_09_004 crossref_primary_10_1007_s40279_012_0012_y crossref_primary_10_1016_S1131_3587_13_70063_3 crossref_primary_10_1016_j_fsigen_2024_103179 crossref_primary_10_3892_etm_2017_4797 crossref_primary_10_1002_asia_201000279 crossref_primary_10_1016_j_copbio_2012_09_004 crossref_primary_10_1111_febs_12252 crossref_primary_10_1186_s12859_021_04516_7 crossref_primary_10_1007_s12185_013_1446_1 crossref_primary_10_1016_j_cca_2018_12_023 crossref_primary_10_1093_cvr_cvv025 crossref_primary_10_1053_j_seminoncol_2011_11_008 crossref_primary_10_1371_journal_pone_0059319 crossref_primary_10_7717_peerj_10017 crossref_primary_10_1016_j_cbd_2021_100902 crossref_primary_10_1186_gm100 crossref_primary_10_5858_arpa_2012_0107_RA crossref_primary_10_1186_s12864_019_6302_0 crossref_primary_10_3390_ijms14035694 crossref_primary_10_1186_s43042_023_00447_5 crossref_primary_10_1186_1471_2105_16_S2_A10 crossref_primary_10_2217_pgs_13_75 crossref_primary_10_1021_acs_jpcb_0c11400 crossref_primary_10_3389_fimmu_2024_1365206 crossref_primary_10_1007_s00774_019_01039_3 crossref_primary_10_1177_20499361241244969 crossref_primary_10_1007_s00109_016_1438_8 crossref_primary_10_1093_nar_gkv677 crossref_primary_10_1111_1541_4337_12618 crossref_primary_10_1002_elps_201200441 crossref_primary_10_3390_v12050550 crossref_primary_10_1016_j_physleta_2017_12_010 crossref_primary_10_1007_s40484_016_0067_0 crossref_primary_10_3390_genes13091566 crossref_primary_10_1039_c2an15930a crossref_primary_10_1016_j_patbio_2009_10_010 crossref_primary_10_1371_journal_pone_0237087 crossref_primary_10_1093_gbe_evaa079 crossref_primary_10_1038_s41598_020_58544_z crossref_primary_10_1097_PAP_0b013e3181c6962f crossref_primary_10_1016_j_joen_2024_07_016 crossref_primary_10_3389_frma_2021_788673 crossref_primary_10_1016_j_compbiolchem_2011_06_001 crossref_primary_10_1161_CIRCGENETICS_113_000486 crossref_primary_10_3389_fnut_2019_00107 crossref_primary_10_1093_bioinformatics_btr349 crossref_primary_10_3389_fmicb_2022_778659 crossref_primary_10_1007_s00705_012_1559_5 crossref_primary_10_1016_j_bios_2018_07_072 crossref_primary_10_1373_clinchem_2016_266247 crossref_primary_10_1016_j_gpb_2012_05_006 crossref_primary_10_1002_pmic_202200335 crossref_primary_10_4061_2011_310135 crossref_primary_10_3390_microorganisms13020244 crossref_primary_10_1038_s41525_020_0115_6 crossref_primary_10_1016_j_jbc_2022_101924 crossref_primary_10_1186_1746_1596_5_23 crossref_primary_10_1002_cpe_5328 crossref_primary_10_1007_s00129_013_3238_3 crossref_primary_10_1371_journal_pone_0186004 crossref_primary_10_1007_s11882_011_0211_x crossref_primary_10_1186_1471_2156_15_28 crossref_primary_10_3390_microorganisms9061307 crossref_primary_10_1186_bcr2431 crossref_primary_10_1186_bcr2791 crossref_primary_10_11144_Javeriana_umed61_2_sngs crossref_primary_10_1371_journal_pone_0056093 crossref_primary_10_1093_humupd_dmq054 crossref_primary_10_1109_TCBB_2018_2858267 crossref_primary_10_15407_ubj85_06_008 crossref_primary_10_1016_j_jmoldx_2016_06_008 crossref_primary_10_1002_humu_21078 crossref_primary_10_1152_physiolgenomics_00044_2013 crossref_primary_10_1093_bfgp_elae031 crossref_primary_10_1016_j_preteyeres_2010_03_004 crossref_primary_10_1093_bfgp_elp040 crossref_primary_10_1016_j_prp_2016_11_016 crossref_primary_10_3390_cancers6010595 crossref_primary_10_1111_cge_12151 crossref_primary_10_1111_apm_12920 crossref_primary_10_1002_humu_22272 crossref_primary_10_1016_j_medcli_2024_10_002 crossref_primary_10_1016_j_compbiomed_2025_109962 crossref_primary_10_1016_j_psiq_2023_100392 crossref_primary_10_35709_ory_2021_58_spl_1 crossref_primary_10_1016_j_cll_2018_11_003 crossref_primary_10_1373_clinchem_2014_225250 crossref_primary_10_1016_j_medcle_2024_10_006 crossref_primary_10_1002_humu_22039 crossref_primary_10_3390_bios13020260 crossref_primary_10_3389_fcimb_2022_987692 crossref_primary_10_1016_j_virusres_2016_12_008 crossref_primary_10_1038_nrneph_2010_18 crossref_primary_10_1053_j_seminoncol_2015_09_026 crossref_primary_10_2217_fmb_10_48 crossref_primary_10_1038_nrgastro_2012_44 crossref_primary_10_1016_j_compbiomed_2020_104017 crossref_primary_10_1080_00365521_2022_2041717 crossref_primary_10_1007_s11560_010_0420_7 crossref_primary_10_1016_j_bdq_2017_10_002 crossref_primary_10_1007_s00040_018_0618_7 crossref_primary_10_1021_ac102188p crossref_primary_10_1016_S1474_4422_19_30033_X crossref_primary_10_1039_C5NR05274B crossref_primary_10_1002_ppul_24159 crossref_primary_10_3389_fmicb_2023_1340427 crossref_primary_10_3390_medsci2020098 crossref_primary_10_3389_fmicb_2021_803190 crossref_primary_10_1007_s00216_016_0112_x crossref_primary_10_1159_000443362 crossref_primary_10_1371_journal_pone_0176185 crossref_primary_10_1039_C9CC04118D crossref_primary_10_1007_s12230_016_9530_8 crossref_primary_10_1186_1471_2105_13_77 crossref_primary_10_1088_1755_1315_482_1_012050 crossref_primary_10_3390_ijms20133193 crossref_primary_10_1016_j_semnephrol_2010_07_002 crossref_primary_10_35118_apjmbb_2024_032_4_Special__08 crossref_primary_10_1021_pr400855q crossref_primary_10_1016_j_pharmthera_2012_08_008 crossref_primary_10_1146_annurev_anchem_061417_125556 crossref_primary_10_1093_bib_bbs013 crossref_primary_10_1002_ece3_8535 crossref_primary_10_3923_ijp_2017_709_723 crossref_primary_10_1264_jsme2_ME20037 crossref_primary_10_2144_000114063 crossref_primary_10_1007_s00414_013_0879_7 crossref_primary_10_1016_j_mib_2009_08_006 crossref_primary_10_1017_S1047951114000754 crossref_primary_10_1016_j_pt_2017_04_004 crossref_primary_10_1021_ol902501j crossref_primary_10_1016_j_bmcl_2009_06_033 crossref_primary_10_1016_j_joen_2022_01_012 crossref_primary_10_22207_JPAM_16_2_45 crossref_primary_10_1021_acs_analchem_9b01887 crossref_primary_10_2174_1381612825666190911155508 crossref_primary_10_1373_clinchem_2014_224360 crossref_primary_10_1038_s41467_021_24001_2 crossref_primary_10_1016_j_cbpc_2011_05_012 crossref_primary_10_1093_gpbjnl_qzae006 crossref_primary_10_1016_j_jbiosc_2013_05_016 crossref_primary_10_1111_tan_12200 crossref_primary_10_1186_1756_0500_5_338 crossref_primary_10_1016_j_ecolind_2019_02_008 crossref_primary_10_4137_BMI_S22433 crossref_primary_10_4155_bio_2016_0186
Cites_doi	10.1073/pnas.0705765104 10.1038/ng.2007.42 10.1038/nature06884 10.1101/gr.078212.108 10.1126/science.1141319 10.1038/nature07517 10.1101/gr.068304.107 10.1002/humu.20749 10.1101/gr.6435207 10.1101/gr.5145806 10.1093/nar/gkn425 10.1093/bioinformatics/btn416 10.1186/1471-2164-9-404 10.1038/ng.259 10.1101/gr.7088808 10.1016/j.tig.2007.12.006 10.1056/NEJMoa073785 10.1038/nature07002 10.1093/bioinformatics/btn025 10.1073/pnas.0710982105 10.1038/nmeth.1223 10.1038/nmeth.1226 10.1038/nmeth.1246 10.1016/j.tibtech.2006.10.005 10.1021/ja0761840 10.1101/gr.081422.108 10.1021/ja057123+ 10.1128/JB.01404-07 10.1038/nature07485 10.1177/154405910808701104 10.1186/1471-2105-9-128 10.1038/nature06745 10.1038/nature07423 10.1038/nature03959 10.1371/journal.pone.0000197 10.1016/j.tibtech.2008.07.003 10.1038/nrg2484 10.1038/nrg2270 10.1371/journal.pone.0002527 10.2144/000112900 10.1101/gr.077776.108 10.1126/science.1158441 10.1186/1471-2164-9-256 10.1038/nmeth1110 10.1038/nmeth1068 10.1101/gr.070227.107 10.1038/nature07509 10.1101/gr.074740.107 10.1038/nbt0798-652 10.1126/science.290.5500.2306 10.1186/gb-2008-9-9-r137 10.1101/gr.075572.107 10.1038/nmeth1109 10.1126/science.1150427 10.1126/science.1149504 10.1038/nprot.2007.520 10.1006/abio.1996.0432 10.1006/abio.1993.1024 10.1101/gr.6468307 10.1038/nrg1982 10.1073/pnas.0605127103 10.1073/pnas.0808319105 10.1093/nar/gni170 10.1056/NEJMoa075463 10.1101/gr.8.3.186 10.1186/gb-2007-8-7-r143 10.1126/science.1117389 10.1021/ac0709394 10.1073/pnas.74.12.5463 10.1126/science.1079700 10.1016/j.cell.2008.03.029 10.1126/science.281.5375.363 10.1101/gr.079558.108 10.1111/j.1567-1364.2008.00434.x 10.1101/gr.074492.107 10.1101/gr.072033.107 10.1073/pnas.0230489100 10.1016/j.cell.2007.05.009 10.1038/nature07484 10.1126/science.1146689 10.1101/gr.7179508 10.1093/nar/gkm566 10.1186/1471-2105-9-431 10.1073/pnas.0810641105 10.1038/nmeth1111 10.1038/ng.128 10.1101/gr.8.3.175 10.1101/gr.6883307 10.1073/pnas.74.2.560 10.1042/BST0361091 10.1101/gr.076463.108 10.1007/s00439-008-0535-3 10.1038/nmeth.1230 10.1038/nmeth.1270 10.1093/bioinformatics/btl629
ContentType	Journal Article
Copyright	2009 INIST-CNRS Copyright American Association for Clinical Chemistry Apr 2009
Copyright_xml	– notice: 2009 INIST-CNRS – notice: Copyright American Association for Clinical Chemistry Apr 2009
DBID	AAYXX CITATION IQODW CGR CUY CVF ECM EIF NPM 3V. 4U- 7QO 7RV 7TM 7U7 7X7 7XB 88E 88I 8AO 8C1 8FD 8FE 8FG 8FI 8FJ 8FK ABJCF ABUWG AEUYN AFKRA AZQEC BENPR BGLVJ BHPHI BKSAR C1K CCPQU D1I DWQXO FR3 FYUFA GHDGH GNUQQ HCIFZ K9. KB. KB0 M0S M1P M2P NAPCQ P64 PCBAR PDBOC PHGZM PHGZT PJZUB PKEHL PPXIY PQEST PQGLB PQQKQ PQUKI Q9U RC3 S0X 7X8
DOI	10.1373/clinchem.2008.112789
DatabaseName	CrossRef Pascal-Francis Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed ProQuest Central (Corporate) University Readers Biotechnology Research Abstracts Nursing & Allied Health Database Nucleic Acids Abstracts Toxicology Abstracts Health & Medical Collection ProQuest Central (purchase pre-March 2016) Medical Database (Alumni Edition) Science Database (Alumni Edition) ProQuest Pharma Collection Public Health Database Technology Research Database ProQuest SciTech Collection ProQuest Technology Collection Hospital Premium Collection Hospital Premium Collection (Alumni Edition) ProQuest Central (Alumni) (purchase pre-March 2016) Materials Science & Engineering Collection ProQuest Central (Alumni) ProQuest One Sustainability ProQuest Central UK/Ireland ProQuest Central Essentials ProQuest Central Technology Collection Natural Science Collection Earth, Atmospheric & Aquatic Science Collection Environmental Sciences and Pollution Management ProQuest One ProQuest Materials Science Collection ProQuest Central Korea Engineering Research Database Health Research Premium Collection Health Research Premium Collection (Alumni) ProQuest Central Student SciTech Premium Collection ProQuest Health & Medical Complete (Alumni) Materials Science Database Nursing & Allied Health Database (Alumni Edition) ProQuest Health & Medical Collection Medical Database Science Database ProQuest Nursing and Allied Health Premium Biotechnology and BioEngineering Abstracts Earth, Atmospheric & Aquatic Science Database Materials Science Collection ProQuest Central Premium ProQuest One Academic ProQuest Health & Medical Research Collection ProQuest One Academic Middle East (New) ProQuest One Health & Nursing ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Applied & Life Sciences ProQuest One Academic ProQuest One Academic UKI Edition ProQuest Central Basic Genetics Abstracts SIRS Editorial MEDLINE - Academic
DatabaseTitle	CrossRef MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) ProQuest Central Student ProQuest Central Essentials Nucleic Acids Abstracts SciTech Premium Collection Environmental Sciences and Pollution Management ProQuest One Applied & Life Sciences ProQuest One Sustainability Health Research Premium Collection Natural Science Collection Health & Medical Research Collection ProQuest Central (New) ProQuest Medical Library (Alumni) ProQuest Science Journals (Alumni Edition) ProQuest One Academic Eastern Edition Earth, Atmospheric & Aquatic Science Database ProQuest Hospital Collection ProQuest Technology Collection Health Research Premium Collection (Alumni) ProQuest Hospital Collection (Alumni) Biotechnology and BioEngineering Abstracts Nursing & Allied Health Premium ProQuest Health & Medical Complete ProQuest One Academic UKI Edition ProQuest Nursing & Allied Health Source (Alumni) Engineering Research Database ProQuest One Academic ProQuest One Academic (New) University Readers Technology Collection Technology Research Database ProQuest One Academic Middle East (New) SIRS Editorial Materials Science Collection ProQuest Health & Medical Complete (Alumni) ProQuest Central (Alumni Edition) ProQuest One Community College ProQuest One Health & Nursing ProQuest Pharma Collection ProQuest Central Earth, Atmospheric & Aquatic Science Collection ProQuest Health & Medical Research Collection Genetics Abstracts Biotechnology Research Abstracts Health and Medicine Complete (Alumni Edition) ProQuest Central Korea Materials Science Database ProQuest Materials Science Collection ProQuest Public Health ProQuest Central Basic Toxicology Abstracts ProQuest Science Journals ProQuest Nursing & Allied Health Source ProQuest SciTech Collection ProQuest Medical Library Materials Science & Engineering Collection ProQuest Central (Alumni) MEDLINE - Academic
DatabaseTitleList	MEDLINE - Academic MEDLINE ProQuest Central Student CrossRef
Database_xml	– sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database – sequence: 3 dbid: 8FG name: ProQuest Technology Collection url: https://search.proquest.com/technologycollection1 sourceTypes: Aggregation Database
DeliveryMethod	fulltext_linktorsrc
Discipline	Medicine
EISSN	1530-8561
EndPage	658
ExternalDocumentID	1985518251 19246620 21325635 10_1373_clinchem_2008_112789 www55_4_641
Genre	Journal Article Review
GroupedDBID	- 1AW 29B 2WC 3V. 4.4 53G 55 5GY 5RE 5VS 5WD 7RV 7X7 88E 88I 8AO 8C1 8FE 8FG 8FH 8FI 8FJ 8R4 8R5 AALRV AAPBV AAPQZ AAQQT ABFLS ABJCF ABOCM ABPPZ ABSAR ABSGY ABUWG ACGOD ACIWK ACPRK ADBBV ADGZP ADIPN AELNO AELWJ AENEX AETBJ AFFNX AFKRA AFRAH AGINJ AGVJH AHMBA AIMBJ AJYGW ALMA_UNASSIGNED_HOLDINGS AZQEC BAWUL BBAFP BCRHZ BENPR BES BGLVJ BHPHI BKEYQ BKSAR BPHCQ BTFSW BVXVI C1A CS3 D1I DIK DU5 DWQXO E3Z EBS EJD ENERS EX3 F5P FHSFR FOTVD FQBLK FYUFA GNUQQ GX1 H13 HCIFZ HH5 H~9 IAO IEA IGS IH2 IHR IHW INH INIJC INR ITC K-O KB. KOP KQ8 L7B LK5 LOTEE LXI M1P M2P M7R NADUK O0- OBOKY OCZFY OK1 PCBAR PDBOC PQEST PQQKQ PQUKI PRINS PROAC PSQYO Q2X R0Z RHF RHI RNS ROX RZL S0X SJN TCC TWZ U5U UCJ UNMZH V8 WH7 WOQ WOW X X7M YXANX ZA5 ZCG ZE2 --- -~X .55 04C 0R~ 18M 6J9 AABZA AACZT AAPXW AARHZ AAUAY AAVAP AAYXX ABCQX ABDFA ABEJV ABGNP ABJNI ABMNT ABNHQ ABPQP ABPTD ABQNK ABSQV ABVGC ABWST ABXVV ABXZS ACIHN ACUTJ ACYHN ADGKP ADNBA ADQBN ADVEK AEAQA AEMQT AEUYN AFFZL AFGWE AFXAL AFYAG AGORE AGQXC AGUTN AHMMS AJBYB AJEEA AJNCP ALIPV ALXQX ATGXG BEYMZ C45 CCPQU CITATION F9R FECEO FLUFQ FOEOM GAUVT HMCUK JXSIZ KBUDW KSI KSN NAPCQ NOMLY NU- OAUYM OJZSN OPAEJ OVD OWPYF PHGZM PHGZT RUSNO TEORI TR2 UKHRP W8F YBU YHG YSK YWH ~V8 .GJ 1KJ AAPGJ AAWDT ABEFU ACFRR ACVCV ACZBC ADMTO AFFQV AGKRT AGMDO AHGBF AI. AJDVS APJGH AQDSO AVNTJ BMSDO EBD EIHBH EIHJH EMOBN IQODW J5H ML- MVM OBFPC PJZUB PPXIY PQGLB PV9 SV3 TMA VH1 YQJ ZGI CGR CUY CVF ECM EIF NPM 4U- 7QO 7TM 7U7 7XB 8FD 8FK C1K FR3 K9. P64 PKEHL Q9U RC3 7X8
ID	FETCH-LOGICAL-c504t-9df9a1fbed603720df6bc0f58ddd0e076ed8188aa99b6b9b15aae11d31b8b7b23
IEDL.DBID	8FG
ISSN	0009-9147 1530-8561
IngestDate	Mon Jul 21 10:16:08 EDT 2025 Sat Aug 23 13:08:07 EDT 2025 Thu Apr 03 07:05:10 EDT 2025 Mon Jul 21 09:11:52 EDT 2025 Tue Jul 01 01:03:55 EDT 2025 Thu Apr 24 22:56:13 EDT 2025 Tue Nov 10 19:19:05 EST 2020
IsDoiOpenAccess	false
IsOpenAccess	true
IsPeerReviewed	true
IsScholarly	true
Issue	4
Keywords	Nucleotide sequence Clinical biology Biochemistry Diagnosis Fundamental research Molecular biology Sequencing
Language	English
License	https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model CC BY 4.0
LinkModel	DirectLink
MergedId	FETCHMERGED-LOGICAL-c504t-9df9a1fbed603720df6bc0f58ddd0e076ed8188aa99b6b9b15aae11d31b8b7b23
Notes	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 ObjectType-Review-3 content type line 23
OpenAccessLink	https://academic.oup.com/clinchem/article-pdf/55/4/641/32674467/clinchem0641.pdf
PMID	19246620
PQID	213983598
PQPubID	47786
PageCount	18
ParticipantIDs	proquest_miscellaneous_67081585 proquest_journals_213983598 pubmed_primary_19246620 pascalfrancis_primary_21325635 crossref_primary_10_1373_clinchem_2008_112789 crossref_citationtrail_10_1373_clinchem_2008_112789 highwire_smallpub1_www55_4_641
ProviderPackageCode	BTFSW RHF RHI CITATION AAYXX
PublicationCentury	2000
PublicationDate	2009-04-01
PublicationDateYYYYMMDD	2009-04-01
PublicationDate_xml	– month: 04 year: 2009 text: 2009-04-01 day: 01
PublicationDecade	2000
PublicationPlace	Washington, DC
PublicationPlace_xml	– name: Washington, DC – name: England – name: Washington
PublicationTitle	Clinical chemistry (Baltimore, Md.)
PublicationTitleAlternate	Clin Chem
PublicationYear	2009
Publisher	Am Assoc Clin Chem American Association for Clinical Chemistry Oxford University Press
Publisher_xml	– name: Am Assoc Clin Chem – name: American Association for Clinical Chemistry – name: Oxford University Press
References	(2020022800253033100_R34) 2007; 318 (2020022800253033100_R51) 2008; 456 (2020022800253033100_R53) 2007; 129 (2020022800253033100_R17) 2005; 309 (2020022800253033100_R77) 2008; 18 (2020022800253033100_R22) 2008; 9 (2020022800253033100_R91) 2007; 8 (2020022800253033100_R96) 2007; 129 (2020022800253033100_R64) 1998; 8 (2020022800253033100_R50) 2008; 40 (2020022800253033100_R92) 2008; 105 (2020022800253033100_R11) 2008; 456 (2020022800253033100_R30) 2007; 4 (2020022800253033100_R38) 2008; 87 (2020022800253033100_R71) 2008; 24 (2020022800253033100_R55) 2008; 18 (2020022800253033100_R23) 2008; 8 (2020022800253033100_R3) 2008; 452 (2020022800253033100_R47) 2008; 45 (2020022800253033100_R68) 2005; 33 (2020022800253033100_R41) 2008; 320 (2020022800253033100_R46) 2008; 18 (2020022800253033100_R61) 2008; 24 (2020022800253033100_R78) 2008; 18 (2020022800253033100_R83) 2007; 35 (2020022800253033100_R82) 2008; 3 (2020022800253033100_R67) 2008; 18 (2020022800253033100_R40) 2009; 10 (2020022800253033100_R15) 2008; 5 (2020022800253033100_R42) 2008; 453 (2020022800253033100_R74) 2008; 18 (2020022800253033100_R45) 2008; 5 (2020022800253033100_R26) 2008; 18 (2020022800253033100_R98) 2006; 24 (2020022800253033100_R25) 2008; 456 (2020022800253033100_R43) 2008; 5 (2020022800253033100_R24) 2008; 456 (2020022800253033100_R33) 2007; 4 (2020022800253033100_R95) 2006; 128 (2020022800253033100_R5) 1996; 242 (2020022800253033100_R94) 2008; 323 (2020022800253033100_R57) 2007; 4 (2020022800253033100_R62) 2008; 9 (2020022800253033100_R21) 2008; 9 (2020022800253033100_R85) 2008; 29 (2020022800253033100_R1) 1977; 74 (2020022800253033100_R14) 2008; 36 (2020022800253033100_R59) 2008; 452 (2020022800253033100_R39) 2008; 457 (2020022800253033100_R27) 2008; 18 (2020022800253033100_R8) 2005; 437 (2020022800253033100_R16) 2008; 5 (2020022800253033100_R84) 2007; 17 (2020022800253033100_R32) 2007; 4 (2020022800253033100_R89) 2007; 79 (2020022800253033100_R29) 2008; 455 (2020022800253033100_R58) 2008; 18 (2020022800253033100_R69) 2007; 23 (2020022800253033100_R72) 2008; 18 (2020022800253033100_R73) 2008; 24 (2020022800253033100_R97) 2008; 26 (2020022800253033100_R2) 1977; 74 (2020022800253033100_R18) 2003; 100 (2020022800253033100_R19) 2008; 320 (2020022800253033100_R54) 2008; 9 (2020022800253033100_R52) 2000; 290 (2020022800253033100_R6) 1998; 281 (2020022800253033100_R90) 2007; 104 (2020022800253033100_R60) 2008; 36 (2020022800253033100_R63) 1998; 8 (2020022800253033100_R65) 2008; 18 (2020022800253033100_R20) 2008; 18 (2020022800253033100_R75) 2007; 17 (2020022800253033100_R87) 2008; 105 (2020022800253033100_R13) 2008; 40 (2020022800253033100_R36) 2008; 3 (2020022800253033100_R28) 2008; 124 (2020022800253033100_R7) 1998; 16 (2020022800253033100_R48) 2008; 18 (2020022800253033100_R10) 2007; 8 (2020022800253033100_R56) 2007; 316 (2020022800253033100_R31) 2007; 39 (2020022800253033100_R12) 2007; 318 (2020022800253033100_R49) 2007; 17 (2020022800253033100_R66) 2008; 9 (2020022800253033100_R70) 2008; 18 (2020022800253033100_R35) 2006; 103 (2020022800253033100_R80) 2008; 9 (2020022800253033100_R44) 2008; 133 (2020022800253033100_R88) 2008; 105 (2020022800253033100_R9) 2007; 189 (2020022800253033100_R93) 2003; 299 (2020022800253033100_R37) 2008; 358 (2020022800253033100_R76) 2008; 18 (2020022800253033100_R86) 2008; 358 (2020022800253033100_R4) 1993; 208 (2020022800253033100_R81) 2007; 2 (2020022800253033100_R79) 2008; 5
References_xml	– volume: 104 start-page: 13116 year: 2007 ident: 2020022800253033100_R90 publication-title: Proc Natl Acad Sci U S A doi: 10.1073/pnas.0705765104 – volume: 39 start-page: 1522 year: 2007 ident: 2020022800253033100_R31 publication-title: Nat Genet doi: 10.1038/ng.2007.42 – volume: 452 start-page: 872 year: 2008 ident: 2020022800253033100_R3 publication-title: Nature doi: 10.1038/nature06884 – volume: 18 start-page: 1851 year: 2008 ident: 2020022800253033100_R67 publication-title: Genome Res doi: 10.1101/gr.078212.108 – volume: 316 start-page: 1497 year: 2007 ident: 2020022800253033100_R56 publication-title: Science doi: 10.1126/science.1141319 – volume: 456 start-page: 53 year: 2008 ident: 2020022800253033100_R11 publication-title: Nature doi: 10.1038/nature07517 – volume: 18 start-page: 751 year: 2008 ident: 2020022800253033100_R27 publication-title: Genome Res doi: 10.1101/gr.068304.107 – volume: 29 start-page: 879 year: 2008 ident: 2020022800253033100_R85 publication-title: Hum Mutat doi: 10.1002/humu.20749 – volume: 17 start-page: 1697 year: 2007 ident: 2020022800253033100_R75 publication-title: Genome Res doi: 10.1101/gr.6435207 – volume: 17 start-page: 69 year: 2007 ident: 2020022800253033100_R49 publication-title: Genome Res doi: 10.1101/gr.5145806 – volume: 36 start-page: e105 year: 2008 ident: 2020022800253033100_R14 publication-title: Nucleic Acids Res doi: 10.1093/nar/gkn425 – volume: 24 start-page: 2431 year: 2008 ident: 2020022800253033100_R71 publication-title: Bioinformatics doi: 10.1093/bioinformatics/btn416 – volume: 9 start-page: 404 year: 2008 ident: 2020022800253033100_R21 publication-title: BMC Genomics doi: 10.1186/1471-2164-9-404 – volume: 40 start-page: 1413 year: 2008 ident: 2020022800253033100_R50 publication-title: Nat Genet doi: 10.1038/ng.259 – volume: 18 start-page: 324 year: 2008 ident: 2020022800253033100_R74 publication-title: Genome Res doi: 10.1101/gr.7088808 – volume: 24 start-page: 142 year: 2008 ident: 2020022800253033100_R61 publication-title: Trends Genet doi: 10.1016/j.tig.2007.12.006 – volume: 358 start-page: 991 year: 2008 ident: 2020022800253033100_R37 publication-title: N Engl J Med doi: 10.1056/NEJMoa073785 – volume: 453 start-page: 1239 year: 2008 ident: 2020022800253033100_R42 publication-title: Nature doi: 10.1038/nature07002 – volume: 24 start-page: 713 year: 2008 ident: 2020022800253033100_R73 publication-title: Bioinformatics doi: 10.1093/bioinformatics/btn025 – volume: 105 start-page: 1176 year: 2008 ident: 2020022800253033100_R92 publication-title: Proc Natl Acad Sci U S A doi: 10.1073/pnas.0710982105 – volume: 5 start-page: 613 year: 2008 ident: 2020022800253033100_R45 publication-title: Nat Methods doi: 10.1038/nmeth.1223 – volume: 5 start-page: 621 year: 2008 ident: 2020022800253033100_R43 publication-title: Nat Methods doi: 10.1038/nmeth.1226 – volume: 5 start-page: 829 year: 2008 ident: 2020022800253033100_R79 publication-title: Nat Methods doi: 10.1038/nmeth.1246 – volume: 24 start-page: 580 year: 2006 ident: 2020022800253033100_R98 publication-title: Trends Biotechnol doi: 10.1016/j.tibtech.2006.10.005 – volume: 129 start-page: 16142 year: 2007 ident: 2020022800253033100_R96 publication-title: J Am Chem Soc doi: 10.1021/ja0761840 – volume: 18 start-page: 1865 year: 2008 ident: 2020022800253033100_R26 publication-title: Genome Res doi: 10.1101/gr.081422.108 – volume: 128 start-page: 1705 year: 2006 ident: 2020022800253033100_R95 publication-title: J Am Chem Soc doi: 10.1021/ja057123+ – volume: 189 start-page: 8402 year: 2007 ident: 2020022800253033100_R9 publication-title: J Bacteriol doi: 10.1128/JB.01404-07 – volume: 456 start-page: 66 year: 2008 ident: 2020022800253033100_R25 publication-title: Nature doi: 10.1038/nature07485 – volume: 87 start-page: 1016 year: 2008 ident: 2020022800253033100_R38 publication-title: J Dent Res doi: 10.1177/154405910808701104 – volume: 9 start-page: 128 year: 2008 ident: 2020022800253033100_R66 publication-title: BMC Bioinformatics doi: 10.1186/1471-2105-9-128 – volume: 452 start-page: 215 year: 2008 ident: 2020022800253033100_R59 publication-title: Nature doi: 10.1038/nature06745 – volume: 455 start-page: 1069 year: 2008 ident: 2020022800253033100_R29 publication-title: Nature doi: 10.1038/nature07423 – volume: 437 start-page: 376 year: 2005 ident: 2020022800253033100_R8 publication-title: Nature doi: 10.1038/nature03959 – volume: 2 start-page: e197 year: 2007 ident: 2020022800253033100_R81 publication-title: PLoS ONE doi: 10.1371/journal.pone.0000197 – volume: 26 start-page: 602 year: 2008 ident: 2020022800253033100_R97 publication-title: Trends Biotechnol doi: 10.1016/j.tibtech.2008.07.003 – volume: 10 start-page: 57 year: 2009 ident: 2020022800253033100_R40 publication-title: Nat Rev Genet doi: 10.1038/nrg2484 – volume: 9 start-page: 179 year: 2008 ident: 2020022800253033100_R54 publication-title: Nat Rev Genet doi: 10.1038/nrg2270 – volume: 3 start-page: e2527 year: 2008 ident: 2020022800253033100_R36 publication-title: PLoS ONE doi: 10.1371/journal.pone.0002527 – volume: 45 start-page: 81 year: 2008 ident: 2020022800253033100_R47 publication-title: Biotechniques doi: 10.2144/000112900 – volume: 18 start-page: 1638 year: 2008 ident: 2020022800253033100_R20 publication-title: Genome Res doi: 10.1101/gr.077776.108 – volume: 320 start-page: 1344 year: 2008 ident: 2020022800253033100_R41 publication-title: Science doi: 10.1126/science.1158441 – volume: 9 start-page: 256 year: 2008 ident: 2020022800253033100_R22 publication-title: BMC Genomics doi: 10.1186/1471-2164-9-256 – volume: 457 start-page: 480 year: 2008 ident: 2020022800253033100_R39 publication-title: Nature – volume: 4 start-page: 931 year: 2007 ident: 2020022800253033100_R33 publication-title: Nat Methods doi: 10.1038/nmeth1110 – volume: 4 start-page: 651 year: 2007 ident: 2020022800253033100_R57 publication-title: Nat Methods doi: 10.1038/nmeth1068 – volume: 18 start-page: 763 year: 2008 ident: 2020022800253033100_R65 publication-title: Genome Res doi: 10.1101/gr.070227.107 – volume: 456 start-page: 470 year: 2008 ident: 2020022800253033100_R51 publication-title: Nature doi: 10.1038/nature07509 – volume: 18 start-page: 957 year: 2008 ident: 2020022800253033100_R78 publication-title: Genome Res doi: 10.1101/gr.074740.107 – volume: 18 start-page: 1638 year: 2008 ident: 2020022800253033100_R72 publication-title: Genome Res doi: 10.1101/gr.077776.108 – volume: 16 start-page: 652 year: 1998 ident: 2020022800253033100_R7 publication-title: Nat Biotechnol doi: 10.1038/nbt0798-652 – volume: 290 start-page: 2306 year: 2000 ident: 2020022800253033100_R52 publication-title: Science doi: 10.1126/science.290.5500.2306 – volume: 9 start-page: R137 year: 2008 ident: 2020022800253033100_R80 publication-title: Genome Biol doi: 10.1186/gb-2008-9-9-r137 – volume: 18 start-page: 1456 year: 2008 ident: 2020022800253033100_R77 publication-title: Genome Res doi: 10.1101/gr.075572.107 – volume: 4 start-page: 907 year: 2007 ident: 2020022800253033100_R32 publication-title: Nat Methods doi: 10.1038/nmeth1109 – volume: 320 start-page: 106 year: 2008 ident: 2020022800253033100_R19 publication-title: Science doi: 10.1126/science.1150427 – volume: 318 start-page: 420 year: 2007 ident: 2020022800253033100_R12 publication-title: Science doi: 10.1126/science.1149504 – volume: 3 start-page: 267 year: 2008 ident: 2020022800253033100_R82 publication-title: Nat Protoc doi: 10.1038/nprot.2007.520 – volume: 242 start-page: 84 year: 1996 ident: 2020022800253033100_R5 publication-title: Anal Biochem doi: 10.1006/abio.1996.0432 – volume: 208 start-page: 171 year: 1993 ident: 2020022800253033100_R4 publication-title: Anal Biochem doi: 10.1006/abio.1993.1024 – volume: 17 start-page: 1195 year: 2007 ident: 2020022800253033100_R84 publication-title: Genome Res doi: 10.1101/gr.6468307 – volume: 8 start-page: 71 year: 2007 ident: 2020022800253033100_R91 publication-title: Nat Rev Genet doi: 10.1038/nrg1982 – volume: 103 start-page: 12115 year: 2006 ident: 2020022800253033100_R35 publication-title: Proc Natl Acad Sci U S A doi: 10.1073/pnas.0605127103 – volume: 105 start-page: 16266 year: 2008 ident: 2020022800253033100_R88 publication-title: Proc Natl Acad Sci U S A doi: 10.1073/pnas.0808319105 – volume: 33 start-page: e171 year: 2005 ident: 2020022800253033100_R68 publication-title: Nucleic Acids Res doi: 10.1093/nar/gni170 – volume: 358 start-page: 1899 year: 2008 ident: 2020022800253033100_R86 publication-title: N Engl J Med doi: 10.1056/NEJMoa075463 – volume: 8 start-page: 186 year: 1998 ident: 2020022800253033100_R63 publication-title: Genome Res doi: 10.1101/gr.8.3.186 – volume: 8 start-page: R143 year: 2007 ident: 2020022800253033100_R10 publication-title: Genome Biol doi: 10.1186/gb-2007-8-7-r143 – volume: 309 start-page: 1728 year: 2005 ident: 2020022800253033100_R17 publication-title: Science doi: 10.1126/science.1117389 – volume: 79 start-page: 7576 year: 2007 ident: 2020022800253033100_R89 publication-title: Anal Chem doi: 10.1021/ac0709394 – volume: 74 start-page: 5463 year: 1977 ident: 2020022800253033100_R2 publication-title: Proc Natl Acad Sci U S A doi: 10.1073/pnas.74.12.5463 – volume: 299 start-page: 682 year: 2003 ident: 2020022800253033100_R93 publication-title: Science doi: 10.1126/science.1079700 – volume: 323 start-page: 133 year: 2008 ident: 2020022800253033100_R94 publication-title: Science – volume: 133 start-page: 523 year: 2008 ident: 2020022800253033100_R44 publication-title: Cell doi: 10.1016/j.cell.2008.03.029 – volume: 281 start-page: 363 year: 1998 ident: 2020022800253033100_R6 publication-title: Science doi: 10.1126/science.281.5375.363 – volume: 18 start-page: 1509 year: 2008 ident: 2020022800253033100_R46 publication-title: Genome Res doi: 10.1101/gr.079558.108 – volume: 8 start-page: 1185 year: 2008 ident: 2020022800253033100_R23 publication-title: FEMS Yeast Res doi: 10.1111/j.1567-1364.2008.00434.x – volume: 18 start-page: 821 year: 2008 ident: 2020022800253033100_R76 publication-title: Genome Res doi: 10.1101/gr.074492.107 – volume: 18 start-page: 802 year: 2008 ident: 2020022800253033100_R70 publication-title: Genome Res doi: 10.1101/gr.072033.107 – volume: 100 start-page: 3960 year: 2003 ident: 2020022800253033100_R18 publication-title: Proc Natl Acad Sci U S A doi: 10.1073/pnas.0230489100 – volume: 129 start-page: 823 year: 2007 ident: 2020022800253033100_R53 publication-title: Cell doi: 10.1016/j.cell.2007.05.009 – volume: 456 start-page: 60 year: 2008 ident: 2020022800253033100_R24 publication-title: Nature doi: 10.1038/nature07484 – volume: 318 start-page: 97 year: 2007 ident: 2020022800253033100_R34 publication-title: Science doi: 10.1126/science.1146689 – volume: 18 start-page: 610 year: 2008 ident: 2020022800253033100_R48 publication-title: Genome Res doi: 10.1101/gr.7179508 – volume: 35 start-page: e97 year: 2007 ident: 2020022800253033100_R83 publication-title: Nucleic Acids Res doi: 10.1093/nar/gkm566 – volume: 9 start-page: 431 year: 2008 ident: 2020022800253033100_R62 publication-title: BMC Bioinformatics doi: 10.1186/1471-2105-9-431 – volume: 105 start-page: 20458 year: 2008 ident: 2020022800253033100_R87 publication-title: Proc Natl Acad Sci U S A doi: 10.1073/pnas.0810641105 – volume: 4 start-page: 903 year: 2007 ident: 2020022800253033100_R30 publication-title: Nat Methods doi: 10.1038/nmeth1111 – volume: 40 start-page: 722 year: 2008 ident: 2020022800253033100_R13 publication-title: Nat Genet doi: 10.1038/ng.128 – volume: 8 start-page: 175 year: 1998 ident: 2020022800253033100_R64 publication-title: Genome Res doi: 10.1101/gr.8.3.175 – volume: 18 start-page: 19 year: 2008 ident: 2020022800253033100_R58 publication-title: Genome Res doi: 10.1101/gr.6883307 – volume: 74 start-page: 560 year: 1977 ident: 2020022800253033100_R1 publication-title: Proc Natl Acad Sci U S A doi: 10.1073/pnas.74.2.560 – volume: 36 start-page: 1091 year: 2008 ident: 2020022800253033100_R60 publication-title: Biochem Soc Trans doi: 10.1042/BST0361091 – volume: 18 start-page: 1051 year: 2008 ident: 2020022800253033100_R55 publication-title: Genome Res doi: 10.1101/gr.076463.108 – volume: 124 start-page: 161 year: 2008 ident: 2020022800253033100_R28 publication-title: Hum Genet doi: 10.1007/s00439-008-0535-3 – volume: 5 start-page: 679 year: 2008 ident: 2020022800253033100_R15 publication-title: Nat Methods doi: 10.1038/nmeth.1230 – volume: 5 start-page: 1005 year: 2008 ident: 2020022800253033100_R16 publication-title: Nat Methods doi: 10.1038/nmeth.1270 – volume: 23 start-page: 500 year: 2007 ident: 2020022800253033100_R69 publication-title: Bioinformatics doi: 10.1093/bioinformatics/btl629
SSID	ssj0004786
Score	2.5063765
SecondaryResourceType	review_article
Snippet	Background: For the past 30 years, the Sanger method has been the dominant approach and gold standard for DNA sequencing. The commercial launch of the first... For the past 30 years, the Sanger method has been the dominant approach and gold standard for DNA sequencing. The commercial launch of the first massively...
SourceID	proquest pubmed pascalfrancis crossref highwire
SourceType	Aggregation Database Index Database Enrichment Source Publisher
StartPage	641
SubjectTerms	Accuracy Analytical, structural and metabolic biochemistry Animals Base Sequence - genetics Bioinformatics Biological and medical sciences Biomedical Research - methods Data processing Deoxyribonucleic acid DNA Fundamental and applied biological sciences. Psychology Genomics Human subjects Humans Industrial development Investigative techniques, diagnostic techniques (general aspects) Life sciences Medical sciences Molecular Diagnostic Techniques - methods Peer Review, Research - methods Software Technological change Validation studies
Title	Next-Generation Sequencing: From Basic Research to Diagnostics
URI	http://www.clinchem.org/cgi/content/abstract/55/4/641 https://www.ncbi.nlm.nih.gov/pubmed/19246620 https://www.proquest.com/docview/213983598 https://www.proquest.com/docview/67081585
Volume	55
hasFullText	1
inHoldings	1
isFullTextHit
isPrint
link	http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwhV1Lb9QwEB7RVkJcEOXV9BFy4GrVTvxIOIDa0qVC6goBlfZm2bFz2t2UZvv_O46dLRyASy5JLGtm7PnGM54P4D2zqm0E74itGCdcekFqrzzphCkRbdDKjGe613N5dcO_LsQi1eYMqaxy2hPHjdr1bTgjPy0RqtSh3dyn218kkEaF5Gpi0NiBPYaOJlR01bMvj9ci1Uj0GGAErmmu0s25SlWn4dohCmUViykRc6jA8_67Z5q6BYdiSTOgvLpIdPF3JDp6pNkLeJ6gZHEWdb8PT_z6JTy9TsnyV_BxHqLa2Fc6iL_4Ecum0Vl9KGZ3_ao4N6ijYiq-KzZ98TlW3oXeza_hZnb58-KKJLoE0grKN6RxXWNYZ72TNHDPuE7alnaids5RT5X0Dr1zbUzTWGkby4QxnjFXMVtbZcvqDeyu-7U_gKJsJcYxwtHWUe6VtaFdaO08xiLeICrIoJpEpdvUSzxQWiz1mCBTlZ4EHGkuo4AzINu_bmMvjf98n09a0MPKLJcobabRQoTQXEuO08j_0M12VDQWhHKVyOBoUpZOa3PQW0vK4N32LS6qkCkxa9_fD1oqREoYSGXwNmr4cb4Yr0pZ0sN_jnwEz6asE2XHsLu5u_cnCF42NocdtVD4rC9YPpprDnvnl_Nv3x8AMAbwAw
linkProvider	ProQuest
linkToHtml	http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV1Lb9QwEB5VRQIuiDdpYesDHKPGiR9JJagKZdnS7l5opd6MHTun3U3bbFXxo_iPjON4Cwfg1HMeimbGM99kHh_AW2pkXXHWpKagLGXC8bR00qUN1zmijazQ_T_d6UxMztjXc36-AT_jLIxvq4w-sXfUtq39P_LdHKFK6dfN7V9cpp40yhdXI4NGsIpj9-MGM7bu_dEhqvddno8_n36apAOpQFrzjK3SyjaVpo1xVmSeocU2wtRZw0trbeYwq3cWY1ipdVUZYSpDudaOUltQUxpp_J4D9Pj3WIGB3A-mj7_cjmHKnljSwxb0IUwOk3qFLHb9mCMqYRGaNxHjSM8r_3skjNuJfXOm7lA_TSDW-Dvy7SPg-DE8GqArOQi29gQ23PIp3J8Oxfln8GHms-iwx9qrm3wLbdoYHPfI-KpdkI8abYLEZj-yaslh6PTzu6Kfw9mdSPIFbC7bpXsFJK8F5k3cZrXNmJPG-PWkpXWY-ziNKCSBIopK1cPuck-hMVd9QU4WKgo40GoGASeQrp-6CLs7_nP_KGpBdQs9n6O0qUKL5FwxJRh-xugP3azfisaJ0LHgCWxHZanBF3RqbbkJ7Kyv4iH2lRm9dO11p4REZIaJWwIvg4ZvvxfzYyHybOufb96BB5PT6Yk6OZodb8PDWPHK6GvYXF1duzcInFZm1Jsrge93fT5-AcIqK6A
linkToPdf	http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV1Lb9QwEB5VrVRxQbxJC1sf4Bitk_iRVCoIuqxaSldIUKk3Y8fOaXdTmq2q_rT-O8ZxvIUDcOo5iWXNy99kxvMBvMmMrCvOmtQUGUuZcDwtnXRpw3WOaIMWuv-nezoTR2fs8zk_34DbeBfGt1XGmNgHatvW_h_5OEeoUvpxc-Nm6Ir4Opm-v_iZegIpX2iNbBrBQk7czTVmb93B8QRV_TbPp5--Hx6lA8FAWnPKVmllm0pnjXFWUM_WYhthatrw0lpLHWb4zuJ5VmpdVUaYymRca5dltshMaaTxMw8w-m_JQpbexcrDu-4SJnuSSQ9hMJ4wOdzaK2Qx9lceUSGL0MiJeEd6jvnfT8U4qdg3auoOddUEko2_o-D-NJw-gocDjCUfgt09hg23fALbp0Oh_im8m_mMOsy09qon30LLNh6U-2R62S7IR432QWLjH1m1ZBK6_vzc6Gdwdi-SfA6by3bpXgLJa4E5FLe0tpQ5aYwfVVpah3mQ04hIEiiiqFQ9zDH3dBpz1RfnZKGigAPFZhBwAun6q4swx-M_74-iFlS30PM5SjtTaJ2cK6YEw22M_tDNelU0VISRBU9gNypLDXGhU2srTmBv_RQd2ldp9NK1V50SElEaJnEJvAgavtsv5spC5HTnnyvvwTZ6hvpyPDvZhQex-EWzV7C5urxyrxFDrcyot1YCP-7bPX4BKRovoQ
openUrl	ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Next-Generation+Sequencing%3A+From+Basic+Research+to+Diagnostics&rft.jtitle=Clinical+chemistry+%28Baltimore%2C+Md.%29&rft.au=Voelkerding%2C+Karl+V&rft.au=Dames%2C+Shale+A&rft.au=Durtschi%2C+Jacob+D&rft.date=2009-04-01&rft.pub=Am+Assoc+Clin+Chem&rft.issn=0009-9147&rft.eissn=1530-8561&rft.volume=55&rft.issue=4&rft.spage=641&rft_id=info:doi/10.1373%2Fclinchem.2008.112789&rft_id=info%3Apmid%2F19246620&rft.externalDBID=n%2Fa&rft.externalDocID=www55_4_641
thumbnail_l	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0009-9147&client=summon
thumbnail_m	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0009-9147&client=summon
thumbnail_s	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0009-9147&client=summon