Bio-based squalene production by Aurantiochytrium sp. through optimization of culture conditions, and elucidation of the putative biosynthetic pathway genes

• Published in: Bioresource technology Vol. 287; p. 121415
• Main Authors: Zhang, Aiqing, Xie, Yunxuan, He, Yaodong, Wang, Weijun, Sen, Biswarup, Wang, Guangyi
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.09.2019
• Subjects: biochemical pathways; bioreactors; Bioresource; biosynthesis; China; coastal water; enzymes; genes; glucose; Mevalonate pathway, RT-qPCR; monosodium glutamate; Orthogonal-design; phylogeny; sodium chloride; squalene; Transcriptome; transcriptomics; yeast extract; China; Bioresource; Mevalonate pathway, RT-qPCR; Orthogonal-design; Transcriptome

•High squalene-producing thraustochytrid strains from coastal waters were identified.•Newly isolated strain Aurantiochytrium sp. TWZ-97 was highest squalene producer.•Strain TWZ-97 produced squalene up to 188.6 mg/L under optimal conditions.•Putative squalene biosynthetic pathway genes identified in...