CRISPR/Cas9-mediated efficient genome editing via blastospore-based transformation in entomopathogenic fungus Beauveria bassiana

• Published in: Scientific reports Vol. 7; no. 1; p. 45763
• Main Authors: Chen, Jingjing, Lai, Yiling, Wang, Lili, Zhai, Suzhen, Zou, Gen, Zhou, Zhihua, Cui, Chunlai, Wang, Sibao
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 03.04.2017; Nature Publishing Group
• Subjects: 42/70; 631/1647/1513/1967/3196; 631/326/193/2484; Agrochemicals; Auxotrophy; Beauveria - genetics; Beauveria - growth & development; Beauveria bassiana; Biological control; CRISPR; CRISPR-Cas Systems; Entomopathogenic fungi; Gene disruption; Gene Editing; Gene Targeting - methods; Genes; Genes, Fungal; Genetic Vectors; Genome editing; Homologous recombination; Humanities and Social Sciences; Humans; Insecticides; multidisciplinary; Mutagenesis; Pests; Recombination, Genetic; Science; Transformation, Genetic; Uracil-DNA Glycosidase - antagonists & inhibitors; Uracil-DNA Glycosidase - genetics; Uridine - metabolism; Vectors
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/srep45763

Beauveria bassiana is an environmentally friendly alternative to chemical insecticides against various agricultural insect pests and vectors of human diseases. However, its application has been limited due to slow kill and sensitivity to abiotic stresses. Understanding of the molecular pathogenesis...