Kar2p availability defines distinct forms of endoplasmic reticulum stress in living cells

• Published in: Molecular biology of the cell Vol. 23; no. 5; pp. 955 - 964
• Main Authors: Lajoie, Patrick, Moir, Robyn D, Willis, Ian M, Snapp, Erik L
• Format: Journal Article
• Language: English
• Published: United States The American Society for Cell Biology 01.03.2012
• Subjects: Dithiothreitol - pharmacology; Endoplasmic Reticulum Stress; Fungal Proteins - genetics; Fungal Proteins - metabolism; Green Fluorescent Proteins - genetics; Green Fluorescent Proteins - metabolism; HSP70 Heat-Shock Proteins - genetics; HSP70 Heat-Shock Proteins - metabolism; Inositol - metabolism; Saccharomyces cerevisiae - drug effects; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - physiology; Tunicamycin - pharmacology; Unfolded Protein Response

Accumulation of misfolded secretory proteins in the endoplasmic reticulum (ER) activates the unfolded protein response (UPR) stress pathway. To enhance secretory protein folding and promote adaptation to stress, the UPR upregulates ER chaperone levels, including BiP. Here we describe chromosomal tag...