Detection by real time PCR of walnut allergen coding sequences in processed foods

• Published in: Food chemistry Vol. 202; pp. 334 - 340
• Main Authors: Linacero, Rosario, Ballesteros, Isabel, Sanchiz, Africa, Prieto, Nuria, Iniesto, Elisa, Martinez, Yolanda, Pedrosa, Mercedes M., Muzquiz, Mercedes, Cabanillas, Beatriz, Rovira, Mercè, Burbano, Carmen, Cuadrado, Carmen
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.07.2016
• Subjects: Allergens - analysis; Allergens - immunology; Antigens, Plant - analysis; Antigens, Plant - immunology; Enzyme-Linked Immunosorbent Assay; Food Analysis - methods; Juglans; Juglans - genetics; Juglans - immunology; Juglans regia; Pressure processing; Processed foods; Real-time PCR; Real-Time Polymerase Chain Reaction - methods; Reproducibility of Results; Thermal processing; Walnut allergen detection; Pressure processing; Ct; HHP; RT; Processed foods; Real-time PCR; Thermal processing; Walnut allergen detection; LTP; NTC; CTAB; Juglans regia

•A qRT-PCR method using walnut allergen coding-sequences was developed.•The method is specific, reliable, and sensitive. LOD, 2.5pg of walnut DNA.•HHP and autoclave treatments can affect DNA quality required for PCR amplification.•The method was accurate and specific for detection of hidden walnut i...