Impaired translation of CCAAT/enhancer binding protein α mRNA in bronchial smooth muscle cells of asthmatic patients
Bronchial smooth muscle (BSM) cells of asthmatic patients have an impaired expression of CCAAT/enhancer binding protein (C/EBP) α, which is associated with increased proliferation. We sought to assess the translational regulation of CEBPA mRNA in cultured BSM cells of healthy control subjects (n = 1...
Saved in:
| Published in | Journal of allergy and clinical immunology Vol. 123; no. 3; pp. 639 - 645 |
|---|---|
| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
United States
Mosby, Inc
01.03.2009
|
| Subjects | |
| Online Access | Get full text |
| ISSN | 0091-6749 1097-6825 1097-6825 |
| DOI | 10.1016/j.jaci.2008.11.006 |
Cover
Loading…
| Abstract | Bronchial smooth muscle (BSM) cells of asthmatic patients have an impaired expression of CCAAT/enhancer binding protein (C/EBP) α, which is associated with increased proliferation.
We sought to assess the translational regulation of
CEBPA mRNA in cultured BSM cells of healthy control subjects (n = 11) and asthmatic patients (n = 12).
Translation efficiency was studied by using a translation control reporter system driven by the control elements present in the
CEBPA mRNA. Translation efficiency was determined by the ratio of 2 artificial hemagglutinin (HA.11) proteins: p23 and p12. We also analyzed levels of proteins that control translation of
CEBPA mRNA, namely heterogeneous nuclear ribonucleoprotein E2, calreticulin, eukaryotic translation initiation factor (eIF4E), and 4E binding protein.
Compared with healthy control subjects, BSM cells of asthmatic patients proliferate faster (2.1-fold) and are primed for IL-6 secretion. Real-time RT-PCR showed that BSM cells of asthmatic patients express normal levels of
CEBPA mRNA, whereas they express lower levels of C/EBPα (p42). Transient transfections with the translation control reporter system construct showed a disturbed p12/p23 ratio in BSM cells of asthmatic patients relative to healthy control subjects, which coincided with lower levels of eIF4E.
BSM cells of asthmatic patients have normal levels of
CEBPA mRNA but inadequately reinitiate the translation into C/EBPα. Impaired translation control upstream of eIF4E might underlie the observed increased proliferation and priming of BSM cells of asthmatic patients. |
|---|---|
| AbstractList | Bronchial smooth muscle (BSM) cells of asthmatic patients have an impaired expression of CCAAT/enhancer binding protein (C/EBP) alpha, which is associated with increased proliferation.BACKGROUNDBronchial smooth muscle (BSM) cells of asthmatic patients have an impaired expression of CCAAT/enhancer binding protein (C/EBP) alpha, which is associated with increased proliferation.We sought to assess the translational regulation of CEBPA mRNA in cultured BSM cells of healthy control subjects (n = 11) and asthmatic patients (n = 12).OBJECTIVEWe sought to assess the translational regulation of CEBPA mRNA in cultured BSM cells of healthy control subjects (n = 11) and asthmatic patients (n = 12).Translation efficiency was studied by using a translation control reporter system driven by the control elements present in the CEBPA mRNA. Translation efficiency was determined by the ratio of 2 artificial hemagglutinin (HA.11) proteins: p23 and p12. We also analyzed levels of proteins that control translation of CEBPA mRNA, namely heterogeneous nuclear ribonucleoprotein E2, calreticulin, eukaryotic translation initiation factor (eIF4E), and 4E binding protein.METHODSTranslation efficiency was studied by using a translation control reporter system driven by the control elements present in the CEBPA mRNA. Translation efficiency was determined by the ratio of 2 artificial hemagglutinin (HA.11) proteins: p23 and p12. We also analyzed levels of proteins that control translation of CEBPA mRNA, namely heterogeneous nuclear ribonucleoprotein E2, calreticulin, eukaryotic translation initiation factor (eIF4E), and 4E binding protein.Compared with healthy control subjects, BSM cells of asthmatic patients proliferate faster (2.1-fold) and are primed for IL-6 secretion. Real-time RT-PCR showed that BSM cells of asthmatic patients express normal levels of CEBPA mRNA, whereas they express lower levels of C/EBPalpha (p42). Transient transfections with the translation control reporter system construct showed a disturbed p12/p23 ratio in BSM cells of asthmatic patients relative to healthy control subjects, which coincided with lower levels of eIF4E.RESULTSCompared with healthy control subjects, BSM cells of asthmatic patients proliferate faster (2.1-fold) and are primed for IL-6 secretion. Real-time RT-PCR showed that BSM cells of asthmatic patients express normal levels of CEBPA mRNA, whereas they express lower levels of C/EBPalpha (p42). Transient transfections with the translation control reporter system construct showed a disturbed p12/p23 ratio in BSM cells of asthmatic patients relative to healthy control subjects, which coincided with lower levels of eIF4E.BSM cells of asthmatic patients have normal levels of CEBPA mRNA but inadequately reinitiate the translation into C/EBPalpha. Impaired translation control upstream of eIF4E might underlie the observed increased proliferation and priming of BSM cells of asthmatic patients.CONCLUSIONBSM cells of asthmatic patients have normal levels of CEBPA mRNA but inadequately reinitiate the translation into C/EBPalpha. Impaired translation control upstream of eIF4E might underlie the observed increased proliferation and priming of BSM cells of asthmatic patients. Bronchial smooth muscle (BSM) cells of asthmatic patients have an impaired expression of CCAAT/enhancer binding protein (C/EBP) α, which is associated with increased proliferation. We sought to assess the translational regulation of CEBPA mRNA in cultured BSM cells of healthy control subjects (n = 11) and asthmatic patients (n = 12). Translation efficiency was studied by using a translation control reporter system driven by the control elements present in the CEBPA mRNA. Translation efficiency was determined by the ratio of 2 artificial hemagglutinin (HA.11) proteins: p23 and p12. We also analyzed levels of proteins that control translation of CEBPA mRNA, namely heterogeneous nuclear ribonucleoprotein E2, calreticulin, eukaryotic translation initiation factor (eIF4E), and 4E binding protein. Compared with healthy control subjects, BSM cells of asthmatic patients proliferate faster (2.1-fold) and are primed for IL-6 secretion. Real-time RT-PCR showed that BSM cells of asthmatic patients express normal levels of CEBPA mRNA, whereas they express lower levels of C/EBPα (p42). Transient transfections with the translation control reporter system construct showed a disturbed p12/p23 ratio in BSM cells of asthmatic patients relative to healthy control subjects, which coincided with lower levels of eIF4E. BSM cells of asthmatic patients have normal levels of CEBPA mRNA but inadequately reinitiate the translation into C/EBPα. Impaired translation control upstream of eIF4E might underlie the observed increased proliferation and priming of BSM cells of asthmatic patients. Bronchial smooth muscle (BSM) cells of asthmatic patients have an impaired expression of CCAAT/enhancer binding protein (C/EBP) alpha, which is associated with increased proliferation. We sought to assess the translational regulation of CEBPA mRNA in cultured BSM cells of healthy control subjects (n = 11) and asthmatic patients (n = 12). Translation efficiency was studied by using a translation control reporter system driven by the control elements present in the CEBPA mRNA. Translation efficiency was determined by the ratio of 2 artificial hemagglutinin (HA.11) proteins: p23 and p12. We also analyzed levels of proteins that control translation of CEBPA mRNA, namely heterogeneous nuclear ribonucleoprotein E2, calreticulin, eukaryotic translation initiation factor (eIF4E), and 4E binding protein. Compared with healthy control subjects, BSM cells of asthmatic patients proliferate faster (2.1-fold) and are primed for IL-6 secretion. Real-time RT-PCR showed that BSM cells of asthmatic patients express normal levels of CEBPA mRNA, whereas they express lower levels of C/EBPalpha (p42). Transient transfections with the translation control reporter system construct showed a disturbed p12/p23 ratio in BSM cells of asthmatic patients relative to healthy control subjects, which coincided with lower levels of eIF4E. BSM cells of asthmatic patients have normal levels of CEBPA mRNA but inadequately reinitiate the translation into C/EBPalpha. Impaired translation control upstream of eIF4E might underlie the observed increased proliferation and priming of BSM cells of asthmatic patients. Background Bronchial smooth muscle (BSM) cells of asthmatic patients have an impaired expression of CCAAT/enhancer binding protein (C/EBP) α, which is associated with increased proliferation. Objective We sought to assess the translational regulation of CEBPA mRNA in cultured BSM cells of healthy control subjects (n = 11) and asthmatic patients (n = 12). Methods Translation efficiency was studied by using a translation control reporter system driven by the control elements present in the CEBPA mRNA. Translation efficiency was determined by the ratio of 2 artificial hemagglutinin (HA.11) proteins: p23 and p12. We also analyzed levels of proteins that control translation of CEBPA mRNA, namely heterogeneous nuclear ribonucleoprotein E2, calreticulin, eukaryotic translation initiation factor (eIF4E), and 4E binding protein. Results Compared with healthy control subjects, BSM cells of asthmatic patients proliferate faster (2.1-fold) and are primed for IL-6 secretion. Real-time RT-PCR showed that BSM cells of asthmatic patients express normal levels of CEBPA mRNA, whereas they express lower levels of C/EBPα (p42). Transient transfections with the translation control reporter system construct showed a disturbed p12/p23 ratio in BSM cells of asthmatic patients relative to healthy control subjects, which coincided with lower levels of eIF4E. Conclusion BSM cells of asthmatic patients have normal levels of CEBPA mRNA but inadequately reinitiate the translation into C/EBPα. Impaired translation control upstream of eIF4E might underlie the observed increased proliferation and priming of BSM cells of asthmatic patients. |
| Author | Baraket, Melissa Miglino, Nicola Tamm, Michael Roth, Michael Borger, Peter Black, Judith L. |
| Author_xml | – sequence: 1 givenname: Peter surname: Borger fullname: Borger, Peter email: pieter.borger@unibas.ch organization: Pulmonary Cell Research, Department of Biomedicine, University Hospital Basel, Basel, Switzerland – sequence: 2 givenname: Nicola surname: Miglino fullname: Miglino, Nicola organization: Pulmonary Cell Research, Department of Biomedicine, University Hospital Basel, Basel, Switzerland – sequence: 3 givenname: Melissa surname: Baraket fullname: Baraket, Melissa organization: Pharmacology, University of Sydney, Sydney, Australia – sequence: 4 givenname: Judith L. surname: Black fullname: Black, Judith L. organization: Pharmacology, University of Sydney, Sydney, Australia – sequence: 5 givenname: Michael surname: Tamm fullname: Tamm, Michael organization: Pulmonary Cell Research, Department of Biomedicine, University Hospital Basel, Basel, Switzerland – sequence: 6 givenname: Michael surname: Roth fullname: Roth, Michael organization: Pulmonary Cell Research, Department of Biomedicine, University Hospital Basel, Basel, Switzerland |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/19121862$$D View this record in MEDLINE/PubMed |
| BookMark | eNqFUl1rFDEUDdJit9U_4IPkybeZJpmdSSIiLIsfhVJB63PIZO64GWeSNckI_Vn-EX-TGbdSKNi-5APOOffec-4pOnLeAUIvKCkpoc35UA7a2JIRIkpKS0KaJ2hFieRFI1h9hFaESFo0fC1P0GmMA8n_Ssin6IRKyqho2ArNF9Ne2wAdTkG7OOpkvcO-x9vtZnN9Dm6nnYGAW-s6677hffAJrMO_f-Hp89UG52cbvDM7q0ccJ-_TDk9zNCNgA-MYFykd027Kwgbv8wkuxWfouNdjhOe39xn6-v7d9fZjcfnpw8V2c1mYmqxTUbVd1Yu-50RzybmuWy2FaKkEwbU0kE3oOmGYpLziuoI1k0K2ci3zmFUr-uoMvTro5rZ_zBCTmmxc-tIO_BxVwwljVS0z8OUtcG4n6NQ-2EmHG_XPqAxgB4AJPsYA_R2EqCUNNaglDbWkoShVOY1MEvdIxqa_Dmez7fgw9c2BCtmenxaCiiZbZ6DLYZmkOm8fpr-9Rzejddbo8TvcQBz8HFw2XlEVmSLqy7Ipy6IQQfK8pM4Cr_8v8Fj1P4hvzog |
| CitedBy_id | crossref_primary_10_18632_oncotarget_7209 crossref_primary_10_1016_j_pupt_2012_05_006 crossref_primary_10_1159_000353427 crossref_primary_10_1371_journal_pone_0081494 crossref_primary_10_1155_2013_875403 crossref_primary_10_1186_s12890_019_0911_x crossref_primary_10_1111_j_1476_5381_2009_00188_x crossref_primary_10_1183_09031936_00174310 crossref_primary_10_1152_ajplung_90624_2008 crossref_primary_10_1183_09031936_00019810 crossref_primary_10_1016_j_imbio_2019_05_006 crossref_primary_10_1074_jbc_M112_373274 crossref_primary_10_1111_j_1365_2222_2009_03270_x crossref_primary_10_1016_j_pupt_2012_08_005 crossref_primary_10_1155_2012_783290 crossref_primary_10_1530_JOE_11_0369 crossref_primary_10_1183_09031936_00013111 crossref_primary_10_34133_2022_9891689 crossref_primary_10_1172_jci_insight_152762 crossref_primary_10_1183_09031936_00068010 crossref_primary_10_1155_2018_9363820 crossref_primary_10_1165_rcmb_2011_0293OC crossref_primary_10_1016_j_pharmthera_2011_02_001 crossref_primary_10_1016_j_cellsig_2016_07_004 crossref_primary_10_1016_S0954_6111_12_70005_7 |
| Cites_doi | 10.1084/jem.20070956 10.1038/ng791 10.1128/MCB.22.20.7242-7257.2002 10.1074/jbc.273.44.28545 10.1161/01.CIR.99.17.2276 10.1067/mai.2002.130047 10.1073/pnas.90.17.8219 10.1038/nprot.2006.274 10.1073/pnas.90.20.9606 10.1101/gad.14.15.1920 10.1152/ajplung.00389.2001 10.1164/rccm.200205-416OC 10.1074/jbc.M411134200 10.1016/j.jaci.2005.03.046 10.1093/nar/gnj029 10.1086/315027 10.1097/01.TP.0000101822.50960.AB 10.1002/jcp.20605 10.1164/rccm.200501-082PP 10.1016/S1471-4914(02)02424-3 10.1164/ajrccm.164.3.2010109 10.1056/NEJMoa021660 10.1042/BST0340012 10.1016/j.jaci.2006.07.056 10.1056/NEJMoa064707 10.1016/S1569-9048(03)00142-3 10.1165/rcmb.2003-0272OC 10.1038/nrc1363 |
| ContentType | Journal Article |
| Copyright | 2009 American Academy of Allergy, Asthma & Immunology American Academy of Allergy, Asthma & Immunology |
| Copyright_xml | – notice: 2009 American Academy of Allergy, Asthma & Immunology – notice: American Academy of Allergy, Asthma & Immunology |
| DBID | AAYXX CITATION CGR CUY CVF ECM EIF NPM 7X8 |
| DOI | 10.1016/j.jaci.2008.11.006 |
| DatabaseName | CrossRef Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed MEDLINE - Academic |
| DatabaseTitle | CrossRef MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) MEDLINE - Academic |
| DatabaseTitleList | MEDLINE - Academic MEDLINE |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Medicine |
| EISSN | 1097-6825 |
| EndPage | 645 |
| ExternalDocumentID | 19121862 10_1016_j_jaci_2008_11_006 S0091674908021805 1_s2_0_S0091674908021805 |
| Genre | Research Support, Non-U.S. Gov't Journal Article |
| GroupedDBID | --- --K --M -~X .1- .55 .FO .GJ .XZ .~1 0R~ 1B1 1P~ 1RT 1~. 1~5 354 3O- 4.4 457 4G. 53G 5GY 5RE 5VS 7-5 71M 8F7 8FE 8FH 8P~ 9JM AAAJQ AABNK AAEDT AAEDW AAFWJ AAIKJ AAKOC AALRI AAOAW AAQFI AAQXK AARKO AATTM AAXKI AAXUO AAYWO ABBQC ABFNM ABJNI ABLJU ABMAC ABMZM ABOCM ABWVN ABXDB ACDAQ ACGFO ACGFS ACIEU ACPRK ACRLP ACRPL ACVFH ADBBV ADCNI ADEZE ADFRT ADMUD ADNMO ADVLN ADXHL AEBSH AEIPS AEKER AENEX AEUPX AFFNX AFJKZ AFPUW AFRAH AFRHN AFTJW AFXIZ AGCQF AGEKW AGHFR AGQPQ AGUBO AGYEJ AHHHB AHMBA AIEXJ AIGII AIIUN AIKHN AITUG AJRQY AJUYK AKBMS AKRWK AKYEP ALMA_UNASSIGNED_HOLDINGS AMRAJ ANKPU ANZVX APXCP ASPBG AVWKF AXJTR AZFZN BKOJK BLXMC BNPGV BPHCQ BVXVI C45 CAG CJTIS COF CS3 DU5 EBS EFJIC EFKBS EJD EO8 EO9 EP2 EP3 EX3 F5P FDB FEDTE FGOYB FIRID FNPLU FYGXN G-2 G-Q GBLVA HDU HMK HMO HVGLF HZ~ IHE J1W J5H K-O KOM L7B LK8 LUGTX M27 M41 MO0 N4W N9A O-L O9- O9~ OAUVE OBH ODZKP OHH OHT OK0 OK1 OVD OZT P-8 P-9 P2P PC. PQQKQ PROAC Q38 R2- ROL RPZ SAE SCC SDF SDG SDP SEL SES SEW SJN SPCBC SSH SSI SSZ T5K TEORI TWZ UGJ UNMZH UV1 WH7 WOW WUQ X7M XFW YOC YQI YQJ Z5R ZGI ZXP ZY1 ~02 ~G- ~KM AACTN RIG AAYXX AGRNS CITATION CGR CUY CVF ECM EIF NPM 7X8 |
| ID | FETCH-LOGICAL-c504t-3bd3f8ff70a7977a5ba988b19e87a9ce016dd8c291737a3e42989b9499383b8f3 |
| IEDL.DBID | .~1 |
| ISSN | 0091-6749 1097-6825 |
| IngestDate | Fri Jul 11 09:45:40 EDT 2025 Thu Apr 03 07:04:39 EDT 2025 Tue Jul 01 04:31:45 EDT 2025 Thu Apr 24 23:03:34 EDT 2025 Sun Apr 06 06:54:18 EDT 2025 Wed Apr 02 07:43:51 EDT 2025 Tue Aug 26 17:58:56 EDT 2025 |
| IsDoiOpenAccess | false |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 3 |
| Keywords | C/EBPα C/EBP eIF4E LP 4EBP bronchial smooth muscle cell hyperplasia Asthma messenger RNA translation PDGF TCRS mTOR uORF HA BSM SP hnRNPE2 Platelet-derived growth factor Heterogeneous nuclear ribonucleoprotein E2 Hemagglutinin Translation control reporter system Long peptide Bronchial smooth muscle Eukaryotic translation initiation factor 4E Short peptide CCAAT/enhancer binding protein 4E binding protein Mammalian target of rapamycin Upstream open reading frame |
| Language | English |
| License | https://www.elsevier.com/tdm/userlicense/1.0 |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c504t-3bd3f8ff70a7977a5ba988b19e87a9ce016dd8c291737a3e42989b9499383b8f3 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| OpenAccessLink | http://www.jacionline.org/article/S0091674908021805/pdf |
| PMID | 19121862 |
| PQID | 67022359 |
| PQPubID | 23479 |
| PageCount | 7 |
| ParticipantIDs | proquest_miscellaneous_67022359 pubmed_primary_19121862 crossref_primary_10_1016_j_jaci_2008_11_006 crossref_citationtrail_10_1016_j_jaci_2008_11_006 elsevier_sciencedirect_doi_10_1016_j_jaci_2008_11_006 elsevier_clinicalkeyesjournals_1_s2_0_S0091674908021805 elsevier_clinicalkey_doi_10_1016_j_jaci_2008_11_006 |
| ProviderPackageCode | CITATION AAYXX |
| PublicationCentury | 2000 |
| PublicationDate | 2009-03-01 |
| PublicationDateYYYYMMDD | 2009-03-01 |
| PublicationDate_xml | – month: 03 year: 2009 text: 2009-03-01 day: 01 |
| PublicationDecade | 2000 |
| PublicationPlace | United States |
| PublicationPlace_xml | – name: United States |
| PublicationTitle | Journal of allergy and clinical immunology |
| PublicationTitleAlternate | J Allergy Clin Immunol |
| PublicationYear | 2009 |
| Publisher | Mosby, Inc |
| Publisher_xml | – name: Mosby, Inc |
| References | Trian, Benard, Bequeret, Rossignol, Girodot, Ghosh (bib6) 2007; 204 Timchenko, Iakova, Welm, Cai, Timchenko (bib27) 2002; 22 Stephens, Li, Jiang, Unruh, Ma (bib2) 2003; 137 Johnson, Roth, Tamm, Hughes, Ge, King (bib5) 2001; 164 Wiesenthal, Leutz, Calkhoven (bib10) 2006; 34 Eickelberg, Roth, Mussmann, Rüdiger, Tamm, Perruchoud (bib15) 1999; 99 Borger, Matsumoto, Boustany, Gencay, Tamm, Burgess (bib9) 2007; 119 WHO/NHLBI Workshop Report (bib1) 1995 Mathews, Sonenberg, Hershey (bib25) 2000 Azolla, Havryk, Chhajed, Hostettler, Black, Johnson (bib28) 2004; 77 Nerlov (bib20) 2004; 4 Miglino, Tamm, Roth, Borger (bib31) 2007; 30 Lin, MacDougald, Diehl, Lane (bib21) 1993; 90 Lekstrom-Himes, Xanthopoulos (bib17) 1998; 273 Calkhoven, Müller, Leutz (bib22) 2002; 8 Grunstein, Veler, Shan, Larson, Grunsetin, Chuang (bib30) 2005; 116 Zhong, Gencay, Bubendorf, Burgess, Parson, Robinson (bib12) 2006; 207 Borger, Black, Roth (bib18) 2002; 110 Chen, Zhao, Rao, Inoue, Hao (bib16) 2005; 280 Halayko, Kartha, Stelmack, McConville, Tam, Camoretti-Mercado (bib29) 2004; 31 Burgess, Johnson, Ge, Au, Poniris, McParland (bib8) 2003; 167 Ossipow, Descombes, Schibler (bib23) 1993; 90 Borger, Black, Tamm, Roth (bib4) 2006; 174 Ma, Cheng, Kong, Wang, Unruh, Stephens (bib7) 2002; 283 Hamilton, Stoneley, Spriggs, Bushell (bib24) 2006; 34 Cox, Thomson, Rubin, Niven, Corris, Siersted (bib3) 2007; 356 Calkhoven, Müller, Leutz (bib19) 2000; 14 Borger, Koëter, Timmerman, Vellenga, Tomee, Kauffman (bib14) 1999; 180 Roth, Johnson, Borger, Bihl, Rüdiger, King (bib13) 2004; 351 Perrotti, Cesi, Trotta, Guerzoni, Santilli, Campbell (bib26) 2002; 30 Wiesenthal, Leutz, Calkhoven (bib11) 2006; 1 Burgess (10.1016/j.jaci.2008.11.006_bib8) 2003; 167 Grunstein (10.1016/j.jaci.2008.11.006_bib30) 2005; 116 Timchenko (10.1016/j.jaci.2008.11.006_bib27) 2002; 22 Perrotti (10.1016/j.jaci.2008.11.006_bib26) 2002; 30 Borger (10.1016/j.jaci.2008.11.006_bib9) 2007; 119 Calkhoven (10.1016/j.jaci.2008.11.006_bib22) 2002; 8 Azolla (10.1016/j.jaci.2008.11.006_bib28) 2004; 77 Lekstrom-Himes (10.1016/j.jaci.2008.11.006_bib17) 1998; 273 Ossipow (10.1016/j.jaci.2008.11.006_bib23) 1993; 90 Hamilton (10.1016/j.jaci.2008.11.006_bib24) 2006; 34 Wiesenthal (10.1016/j.jaci.2008.11.006_bib11) 2006; 1 Eickelberg (10.1016/j.jaci.2008.11.006_bib15) 1999; 99 Lin (10.1016/j.jaci.2008.11.006_bib21) 1993; 90 Wiesenthal (10.1016/j.jaci.2008.11.006_bib10) 2006; 34 Borger (10.1016/j.jaci.2008.11.006_bib18) 2002; 110 Zhong (10.1016/j.jaci.2008.11.006_bib12) 2006; 207 Borger (10.1016/j.jaci.2008.11.006_bib14) 1999; 180 Nerlov (10.1016/j.jaci.2008.11.006_bib20) 2004; 4 Roth (10.1016/j.jaci.2008.11.006_bib13) 2004; 351 Borger (10.1016/j.jaci.2008.11.006_bib4) 2006; 174 Miglino (10.1016/j.jaci.2008.11.006_bib31) 2007; 30 Trian (10.1016/j.jaci.2008.11.006_bib6) 2007; 204 Chen (10.1016/j.jaci.2008.11.006_bib16) 2005; 280 Calkhoven (10.1016/j.jaci.2008.11.006_bib19) 2000; 14 Mathews (10.1016/j.jaci.2008.11.006_bib25) 2000 Halayko (10.1016/j.jaci.2008.11.006_bib29) 2004; 31 Ma (10.1016/j.jaci.2008.11.006_bib7) 2002; 283 Stephens (10.1016/j.jaci.2008.11.006_bib2) 2003; 137 WHO/NHLBI Workshop Report (10.1016/j.jaci.2008.11.006_bib1) 1995 Johnson (10.1016/j.jaci.2008.11.006_bib5) 2001; 164 Cox (10.1016/j.jaci.2008.11.006_bib3) 2007; 356 |
| References_xml | – volume: 90 start-page: 8219 year: 1993 end-page: 8223 ident: bib23 article-title: CCAAT/enhancer-binding protein mRNA is translated into multiple proteins with different transcription activation potentials publication-title: Proc Natl Acad Sci U S A – volume: 273 start-page: 28545 year: 1998 end-page: 28548 ident: bib17 article-title: Biological role of the CCAAT/enhancer-binding protein family of transcription factors publication-title: J Biol Chem – volume: 356 start-page: 1327 year: 2007 end-page: 1337 ident: bib3 article-title: Asthma control during the year after bronchial thermoplasty publication-title: N Engl J Med – volume: 110 start-page: 841 year: 2002 end-page: 846 ident: bib18 article-title: Asthma and the CCAAT-enhancer binding proteins: a holistic view on airway inflammation and remodeling publication-title: J Allergy Clin Immunol – volume: 119 start-page: 98 year: 2007 end-page: 105 ident: bib9 article-title: Disease specific expression and regulation of CCAAT/enhancer binding proteins in asthma and COPD publication-title: J Allergy Clin Immunol – volume: 22 start-page: 7242 year: 2002 end-page: 7257 ident: bib27 article-title: Calreticulin interacts with C/EBPα and C/EBPβ mRNAs and represses translation of C/EBP proteins publication-title: Mol Cell Biol – volume: 34 start-page: e23 year: 2006 ident: bib10 article-title: A translation control reporter system (TCRS) for the analysis of translationally controlled processes in the vertebrate cell publication-title: Nucleic Acids Res – volume: 283 start-page: L1181 year: 2002 end-page: L1189 ident: bib7 article-title: Changes in biophysical and biochemical properties of single bronchial smooth muscle cells from asthmatic subjects publication-title: Am J Physiol Lung Cell Mol Physiol – volume: 30 start-page: e1667 year: 2007 ident: bib31 article-title: House dust mite allergens may induce ASM remodeling/hyperplasia by reducing C/EBPα levels publication-title: Eur Respir J – volume: 1 start-page: 1531 year: 2006 end-page: 1537 ident: bib11 article-title: Analysis of translation initiation using a translation control reporter system publication-title: Nat Protocol – year: 1995 ident: bib1 article-title: Global strategy for asthma management and prevention – volume: 180 start-page: 1267 year: 1999 end-page: 1274 ident: bib14 article-title: Proteases from publication-title: J Infect Dis – volume: 4 start-page: 394 year: 2004 end-page: 400 ident: bib20 article-title: C/EBPα mutations in acute myeloid leukemias publication-title: Nat Rev Cancer – volume: 31 start-page: 266 year: 2004 end-page: 275 ident: bib29 article-title: Phophatidylinositol-3 kinase/mammalian target of rapamycin/p70S6K regulates contractile protein accumulation in airway myocyte differentiation publication-title: Am J Respir Cell Mol Biol – volume: 30 start-page: 48 year: 2002 end-page: 58 ident: bib26 article-title: BCR-ABL suppresses C/EBPα expression through inhibitory action of hnRNP E2 publication-title: Nat Genet – volume: 137 start-page: 125 year: 2003 end-page: 140 ident: bib2 article-title: The biophysics of asthmatic airway smooth muscle publication-title: Respir Physiol Neurobiol – volume: 351 start-page: 560 year: 2004 end-page: 574 ident: bib13 article-title: Dysfunctional interaction of C/EBPα and the glucocorticoid receptor in asthmatic bronchial smooth-muscle cells publication-title: N Engl J Med – volume: 99 start-page: 2276 year: 1999 end-page: 2282 ident: bib15 article-title: Calcium channel blockers activate the interleukin-6 gene via the transcription factors NF-IL6 and NF-kappaB in primary human vascular smooth muscle cells publication-title: Circulation – volume: 207 start-page: 540 year: 2006 end-page: 552 ident: bib12 article-title: ERK1/2 and p38 MAP kinase control MMP-2, MT1-MMP, and TIMP action and affect cell migration: a comparison between mesothelioma and mesothelial cells publication-title: J Cell Physiol – volume: 204 start-page: 3173 year: 2007 end-page: 3181 ident: bib6 article-title: Bronchial smooth muscle remodeling involves calcium-dependent enhanced mitochondrial biogenesis in asthma publication-title: J Exp Med – year: 2000 ident: bib25 article-title: Origins and principles of translation control. Translational control of gene expression – volume: 8 start-page: 577 year: 2002 end-page: 583 ident: bib22 article-title: Translational control of gene expression and disease publication-title: Trends Mol Med – volume: 116 start-page: 94 year: 2005 end-page: 101 ident: bib30 article-title: Proasthmatic effects and mechanisms of action of the dust mite allergen, Der p 1, in airway smooth muscle publication-title: J Allergy Clin Immunol – volume: 167 start-page: 71 year: 2003 end-page: 77 ident: bib8 article-title: Expression of connective tissue growth factor in asthmatic airway smooth muscle cells publication-title: Am J Respir Crit Care Med – volume: 77 start-page: 275 year: 2004 end-page: 280 ident: bib28 article-title: Everolimus and mycophenolate mofetil are potent inhibitors of fibroblast proliferation after lung transplantation publication-title: Transplantation – volume: 34 start-page: 12 year: 2006 end-page: 16 ident: bib24 article-title: TOPs and their regulation publication-title: Biochem Soc – volume: 174 start-page: 367 year: 2006 end-page: 372 ident: bib4 article-title: Asthma—is it due to an abnormal airway smooth muscle cell? publication-title: Am J Respir Crit Care Med – volume: 280 start-page: 16354 year: 2005 end-page: 16359 ident: bib16 article-title: C/EBPβ and its binding element are required for NFκB-induced COX2 expression following hypertonic stress publication-title: J Biol Chem – volume: 90 start-page: 9606 year: 1993 end-page: 9610 ident: bib21 article-title: A 30-kDa alternative translation product of the CCAAT/enhancer binding protein alpha message: transcriptional activator lacking antimitotic activity publication-title: Proc Natl Acad Sci U S A – volume: 164 start-page: 474 year: 2001 end-page: 477 ident: bib5 article-title: Airway smooth muscle cell proliferation is increased in asthma publication-title: Am J Respir Crit Care Med – volume: 14 start-page: 1920 year: 2000 end-page: 1932 ident: bib19 article-title: Translational control of C/EBPα and C/EBPβ isoform expression publication-title: Genes Dev – volume: 204 start-page: 3173 year: 2007 ident: 10.1016/j.jaci.2008.11.006_bib6 article-title: Bronchial smooth muscle remodeling involves calcium-dependent enhanced mitochondrial biogenesis in asthma publication-title: J Exp Med doi: 10.1084/jem.20070956 – volume: 30 start-page: 48 year: 2002 ident: 10.1016/j.jaci.2008.11.006_bib26 article-title: BCR-ABL suppresses C/EBPα expression through inhibitory action of hnRNP E2 publication-title: Nat Genet doi: 10.1038/ng791 – volume: 22 start-page: 7242 year: 2002 ident: 10.1016/j.jaci.2008.11.006_bib27 article-title: Calreticulin interacts with C/EBPα and C/EBPβ mRNAs and represses translation of C/EBP proteins publication-title: Mol Cell Biol doi: 10.1128/MCB.22.20.7242-7257.2002 – volume: 273 start-page: 28545 year: 1998 ident: 10.1016/j.jaci.2008.11.006_bib17 article-title: Biological role of the CCAAT/enhancer-binding protein family of transcription factors publication-title: J Biol Chem doi: 10.1074/jbc.273.44.28545 – volume: 99 start-page: 2276 year: 1999 ident: 10.1016/j.jaci.2008.11.006_bib15 article-title: Calcium channel blockers activate the interleukin-6 gene via the transcription factors NF-IL6 and NF-kappaB in primary human vascular smooth muscle cells publication-title: Circulation doi: 10.1161/01.CIR.99.17.2276 – volume: 110 start-page: 841 year: 2002 ident: 10.1016/j.jaci.2008.11.006_bib18 article-title: Asthma and the CCAAT-enhancer binding proteins: a holistic view on airway inflammation and remodeling publication-title: J Allergy Clin Immunol doi: 10.1067/mai.2002.130047 – volume: 90 start-page: 8219 year: 1993 ident: 10.1016/j.jaci.2008.11.006_bib23 article-title: CCAAT/enhancer-binding protein mRNA is translated into multiple proteins with different transcription activation potentials publication-title: Proc Natl Acad Sci U S A doi: 10.1073/pnas.90.17.8219 – volume: 1 start-page: 1531 year: 2006 ident: 10.1016/j.jaci.2008.11.006_bib11 article-title: Analysis of translation initiation using a translation control reporter system publication-title: Nat Protocol doi: 10.1038/nprot.2006.274 – volume: 90 start-page: 9606 year: 1993 ident: 10.1016/j.jaci.2008.11.006_bib21 article-title: A 30-kDa alternative translation product of the CCAAT/enhancer binding protein alpha message: transcriptional activator lacking antimitotic activity publication-title: Proc Natl Acad Sci U S A doi: 10.1073/pnas.90.20.9606 – volume: 14 start-page: 1920 year: 2000 ident: 10.1016/j.jaci.2008.11.006_bib19 article-title: Translational control of C/EBPα and C/EBPβ isoform expression publication-title: Genes Dev doi: 10.1101/gad.14.15.1920 – volume: 283 start-page: L1181 year: 2002 ident: 10.1016/j.jaci.2008.11.006_bib7 article-title: Changes in biophysical and biochemical properties of single bronchial smooth muscle cells from asthmatic subjects publication-title: Am J Physiol Lung Cell Mol Physiol doi: 10.1152/ajplung.00389.2001 – volume: 167 start-page: 71 year: 2003 ident: 10.1016/j.jaci.2008.11.006_bib8 article-title: Expression of connective tissue growth factor in asthmatic airway smooth muscle cells publication-title: Am J Respir Crit Care Med doi: 10.1164/rccm.200205-416OC – volume: 280 start-page: 16354 year: 2005 ident: 10.1016/j.jaci.2008.11.006_bib16 article-title: C/EBPβ and its binding element are required for NFκB-induced COX2 expression following hypertonic stress publication-title: J Biol Chem doi: 10.1074/jbc.M411134200 – year: 1995 ident: 10.1016/j.jaci.2008.11.006_bib1 – volume: 116 start-page: 94 year: 2005 ident: 10.1016/j.jaci.2008.11.006_bib30 article-title: Proasthmatic effects and mechanisms of action of the dust mite allergen, Der p 1, in airway smooth muscle publication-title: J Allergy Clin Immunol doi: 10.1016/j.jaci.2005.03.046 – volume: 34 start-page: e23 year: 2006 ident: 10.1016/j.jaci.2008.11.006_bib10 article-title: A translation control reporter system (TCRS) for the analysis of translationally controlled processes in the vertebrate cell publication-title: Nucleic Acids Res doi: 10.1093/nar/gnj029 – volume: 180 start-page: 1267 year: 1999 ident: 10.1016/j.jaci.2008.11.006_bib14 article-title: Proteases from Aspergillus fumigatus induce interleukin (IL)-6 and IL-8 production in airway epithelial cell lines by transcriptional mechanisms publication-title: J Infect Dis doi: 10.1086/315027 – volume: 77 start-page: 275 year: 2004 ident: 10.1016/j.jaci.2008.11.006_bib28 article-title: Everolimus and mycophenolate mofetil are potent inhibitors of fibroblast proliferation after lung transplantation publication-title: Transplantation doi: 10.1097/01.TP.0000101822.50960.AB – volume: 207 start-page: 540 year: 2006 ident: 10.1016/j.jaci.2008.11.006_bib12 article-title: ERK1/2 and p38 MAP kinase control MMP-2, MT1-MMP, and TIMP action and affect cell migration: a comparison between mesothelioma and mesothelial cells publication-title: J Cell Physiol doi: 10.1002/jcp.20605 – volume: 174 start-page: 367 year: 2006 ident: 10.1016/j.jaci.2008.11.006_bib4 article-title: Asthma—is it due to an abnormal airway smooth muscle cell? publication-title: Am J Respir Crit Care Med doi: 10.1164/rccm.200501-082PP – volume: 8 start-page: 577 year: 2002 ident: 10.1016/j.jaci.2008.11.006_bib22 article-title: Translational control of gene expression and disease publication-title: Trends Mol Med doi: 10.1016/S1471-4914(02)02424-3 – volume: 164 start-page: 474 year: 2001 ident: 10.1016/j.jaci.2008.11.006_bib5 article-title: Airway smooth muscle cell proliferation is increased in asthma publication-title: Am J Respir Crit Care Med doi: 10.1164/ajrccm.164.3.2010109 – volume: 351 start-page: 560 year: 2004 ident: 10.1016/j.jaci.2008.11.006_bib13 article-title: Dysfunctional interaction of C/EBPα and the glucocorticoid receptor in asthmatic bronchial smooth-muscle cells publication-title: N Engl J Med doi: 10.1056/NEJMoa021660 – volume: 34 start-page: 12 year: 2006 ident: 10.1016/j.jaci.2008.11.006_bib24 article-title: TOPs and their regulation publication-title: Biochem Soc doi: 10.1042/BST0340012 – volume: 30 start-page: e1667 issue: suppl 51 year: 2007 ident: 10.1016/j.jaci.2008.11.006_bib31 article-title: House dust mite allergens may induce ASM remodeling/hyperplasia by reducing C/EBPα levels publication-title: Eur Respir J – volume: 119 start-page: 98 year: 2007 ident: 10.1016/j.jaci.2008.11.006_bib9 article-title: Disease specific expression and regulation of CCAAT/enhancer binding proteins in asthma and COPD publication-title: J Allergy Clin Immunol doi: 10.1016/j.jaci.2006.07.056 – volume: 356 start-page: 1327 year: 2007 ident: 10.1016/j.jaci.2008.11.006_bib3 article-title: Asthma control during the year after bronchial thermoplasty publication-title: N Engl J Med doi: 10.1056/NEJMoa064707 – volume: 137 start-page: 125 year: 2003 ident: 10.1016/j.jaci.2008.11.006_bib2 article-title: The biophysics of asthmatic airway smooth muscle publication-title: Respir Physiol Neurobiol doi: 10.1016/S1569-9048(03)00142-3 – volume: 31 start-page: 266 year: 2004 ident: 10.1016/j.jaci.2008.11.006_bib29 article-title: Phophatidylinositol-3 kinase/mammalian target of rapamycin/p70S6K regulates contractile protein accumulation in airway myocyte differentiation publication-title: Am J Respir Cell Mol Biol doi: 10.1165/rcmb.2003-0272OC – year: 2000 ident: 10.1016/j.jaci.2008.11.006_bib25 – volume: 4 start-page: 394 year: 2004 ident: 10.1016/j.jaci.2008.11.006_bib20 article-title: C/EBPα mutations in acute myeloid leukemias publication-title: Nat Rev Cancer doi: 10.1038/nrc1363 |
| SSID | ssj0009389 |
| Score | 2.067691 |
| Snippet | Bronchial smooth muscle (BSM) cells of asthmatic patients have an impaired expression of CCAAT/enhancer binding protein (C/EBP) α, which is associated with... Background Bronchial smooth muscle (BSM) cells of asthmatic patients have an impaired expression of CCAAT/enhancer binding protein (C/EBP) α, which is... Bronchial smooth muscle (BSM) cells of asthmatic patients have an impaired expression of CCAAT/enhancer binding protein (C/EBP) alpha, which is associated with... |
| SourceID | proquest pubmed crossref elsevier |
| SourceType | Aggregation Database Index Database Enrichment Source Publisher |
| StartPage | 639 |
| SubjectTerms | Allergy and Immunology Asthma Asthma - genetics Asthma - immunology Asthma - metabolism Bronchi - immunology Bronchi - metabolism bronchial smooth muscle cell hyperplasia C/EBPα Calreticulin - immunology Calreticulin - metabolism CCAAT-Enhancer-Binding Protein-alpha - biosynthesis CCAAT-Enhancer-Binding Protein-alpha - immunology Cell Proliferation Cells, Cultured Eukaryotic Initiation Factor-4E - immunology Eukaryotic Initiation Factor-4E - metabolism Heterogeneous-Nuclear Ribonucleoproteins - immunology Heterogeneous-Nuclear Ribonucleoproteins - metabolism Humans Interleukin-6 - biosynthesis Interleukin-6 - immunology messenger RNA translation Myocytes, Smooth Muscle - immunology Myocytes, Smooth Muscle - metabolism Protein Biosynthesis RNA, Messenger - immunology RNA, Messenger - metabolism |
| Title | Impaired translation of CCAAT/enhancer binding protein α mRNA in bronchial smooth muscle cells of asthmatic patients |
| URI | https://www.clinicalkey.com/#!/content/1-s2.0-S0091674908021805 https://www.clinicalkey.es/playcontent/1-s2.0-S0091674908021805 https://dx.doi.org/10.1016/j.jaci.2008.11.006 https://www.ncbi.nlm.nih.gov/pubmed/19121862 https://www.proquest.com/docview/67022359 |
| Volume | 123 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3NTtwwELYQSFUvVenvUkp96K1KN1nH2D5GK9DSij1UIHGzYscRQWyC1tlr34kX4Zk6EzugqpRKvUWRHcee8cw4-eYbQj5zAWG4sTZJq0omucpUopizCbSuBee1MhIThU-Xh4vz_NsFv9gi8zEXBmGV0fYHmz5Y63hnGldzetM0mOOrEEKvMFs0kwOPKbLXgU5__fkA81BMhhBYZQm2jokzAeN1Vdom4CmRyROrHj3unP4WfA5O6PgleRGjR1qEF9wlW659RZ6dxv_jr8nmBHY3TKiiPfqggHOjXU3n86I4m7r2EoW8pqYZklnowNLQtPTulq5-LAsKl2bdtYh_vqZ-1YEY6WrjYSyKX_g9Pqr0_eXA80ojJ6t_Q86Pj87miyQWVkgsT_M-YaZitaxrkZYC4r-Sm1JJaTLlpCiVdbA4IDk7g6McEyVzOdK0G6SxgfOskTV7S7bbrnXvCa0EV84gCV8u88pinqu1ua1k6owsczYh2bii2kbWcSx-ca1HeNmVRimEcphwGAEpTMiX-z43gXPjydZsFJQes0nB_mlwCU_2Eo_1cj5uYa8z7Wc61X-o2YTw-56_aeo_R_w0apGGLYxSK1vXbbw-FBBIMa4m5F1QrodZqwxrhs32_nPMD-R5-PuFmLl9st2vN-4jBFG9ORh2yQHZKU6-L5a_ANVvG1M |
| linkProvider | Elsevier |
| linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3NatwwEBZhA20vJf3fpm106K2YtVdyLB3N0rDbZPdQNpCbkGSZOGTtsPY-WF4kz9QZS04oTVPozRiPZWl-Zc18Q8jXNIMw3FgbxUUhIi4TGUnmbARPl1maltIILBRero7n5_zHRXqxR2ZDLQymVQbb7216b63DnUlYzclNVWGNr8QUeonVoolAHNN9RKfiI7KfL07nqwfsXSZ8FCyTCAlC7YxP87rStvIplQjmiY2PHvdPf4s_ez90ckBehgCS5v4bX5E9V78mz5bhiPwN2S1AwWFOBe3QDflUN9qUdDbL8_XE1ZfI5y01VV_PQnughqqmd7d083OVU7g026bGFOhr2m4a4CTd7FoYi-JP_hZfpdvusod6pQGWtX1Lzk--r2fzKPRWiGwa8y5ipmClKMss1hmEgDo1WgphEulEpqV1sDjAPDuF3RzLNHMckdoNItnAltaIkr0jo7qp3QdCiyyVziAOHxe8sFjqai23hYidEZqzMUmGFVU2AI9j_4trNWSYXSnkgu-ICfsR4MKYfLunufGwG08-zQZGqaGgFEygAq_wJFX2GJVrgxa3KlHtVMXqD0kbk_Se8jdh_eeIR4MUKdBi5JquXbNr1XEGsRRL5Zi898L1MGuZYNuw6cf_HPOIPJ-vl2fqbLE6PSQv_GEYptB9IqNuu3OfIabqzJegM78Ae2weBA |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Impaired+translation+of+CCAAT%2Fenhancer+binding+protein+alpha+mRNA+in+bronchial+smooth+muscle+cells+of+asthmatic+patients&rft.jtitle=Journal+of+allergy+and+clinical+immunology&rft.au=Borger%2C+Peter&rft.au=Miglino%2C+Nicola&rft.au=Baraket%2C+Melissa&rft.au=Black%2C+Judith+L&rft.date=2009-03-01&rft.issn=1097-6825&rft.eissn=1097-6825&rft.volume=123&rft.issue=3&rft.spage=639&rft_id=info:doi/10.1016%2Fj.jaci.2008.11.006&rft.externalDBID=NO_FULL_TEXT |
| thumbnail_m | http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=https%3A%2F%2Fcdn.clinicalkey.com%2Fck-thumbnails%2F00916749%2FS0091674909X00035%2Fcov150h.gif |