Loss of the respiratory enzyme citrate synthase directly links the Warburg effect to tumor malignancy

• Published in: Scientific reports Vol. 2; no. 1; p. 785
• Main Authors: Lin, Chin-Chih, Cheng, Tsung-Lin, Tsai, Wen-Hui, Tsai, Hui-Ju, Hu, Keng-Hsun, Chang, Hao-Chun, Yeh, Chin-Wei, Chen, Ying-Chou, Liao, Ching-Chun, Chang, Wen-Tsan
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 08.11.2012; Nature Publishing Group
• Subjects: 631/208/191/505; 631/45/320; 631/67/70; 631/80/641/83; Adenosine Triphosphate - pharmacology; Animals; Apoptosis Regulatory Proteins; Carrier Proteins - metabolism; Cell Line, Tumor; Cervical cancer; Citrate (si)-Synthase - antagonists & inhibitors; Citrate (si)-Synthase - genetics; Citrate (si)-Synthase - metabolism; Deregulation; Disease Progression; Energy Metabolism - drug effects; Enzymes; Epithelial-Mesenchymal Transition - drug effects; Female; HeLa Cells; Humanities and Social Sciences; Humans; Intracellular Signaling Peptides and Proteins - metabolism; Leupeptins - pharmacology; Metabolism; Mice; Mice, Inbred NOD; Mitochondrial Proteins - metabolism; multidisciplinary; p53 Protein; Phenotype; Physical characteristics; Proteasome Endopeptidase Complex - chemistry; Proteasome Endopeptidase Complex - metabolism; Proteasomes; Proto-Oncogene Proteins c-mdm2 - antagonists & inhibitors; Proto-Oncogene Proteins c-mdm2 - genetics; Proto-Oncogene Proteins c-mdm2 - metabolism; RNA Interference; RNA, Small Interfering - metabolism; Science; Signal transduction; Signal Transduction - drug effects; Transplantation, Heterologous; Tumor Suppressor Protein p53 - metabolism; Tumors; Uterine Cervical Neoplasms - metabolism; Uterine Cervical Neoplasms - pathology; Xenografts
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/srep00785

To investigate whether altered energy metabolism induces the Warburg effect and results in tumor malignancy, the respiratory enzyme citrate synthase (CS) was examined, silenced and the effects analyzed. In human cervical carcinoma cells, RNAi-mediated CS knockdown induced morphological changes chara...