Determination of Pesticide Residue Cartap Using a Sensitive Fluorescent Probe
The insecticide cartap （CP） is non-fluorescent in aqueous solutions. This property makes its determination through direct fluorescent method difficult. In acidic medium and at room temperature, palmatine （PAL） can react with cucurbit[7]uril （CB[7]） to form stable complexes, and the fluorescence inte...
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| Published in | Journal of Integrative Agriculture Vol. 11; no. 11; pp. 1861 - 1870 |
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| Main Authors | , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Elsevier B.V
01.11.2012
Science Press Analytical and Testing Center, Shanxi Normal University, Linfen 041000, P.R.China KeAi Communications Co., Ltd |
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| Abstract | The insecticide cartap （CP） is non-fluorescent in aqueous solutions. This property makes its determination through direct fluorescent method difficult. In acidic medium and at room temperature, palmatine （PAL） can react with cucurbit[7]uril （CB[7]） to form stable complexes, and the fluorescence intensity of the complex is greatly enhanced. Significant quenching of the fluorescence intensity of the CB[7]-PAL complex was observed with the addition of cartap. Based on the significant quenching of the supramolecular complex fluorescence intensity, a new spectrofluorimetric method with high sensitivity and selectivity was developed to determine cartap in aqueous solution. The fluorescence quenching values （AF） showed good linear relationship with cartap concentrations from 0.009 to 2.4 ~tg mL-~ with a detection limit 0.0029 ~tg mE-x. The proposed method had been successfully applied to the determination of cartap residues in grain and vegetable with recoveries of 87.4-103%. In addition, the association constants of the complexes formed between the host and the guest were determined. The competing reaction and the supramolecular interaction mechanisms between the cartap and PAL as they fight for occupancy of the CB[7] cavity were studied using spectrofluorimetry, xH NMR and molecular modeling calculations. |
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| AbstractList | The insecticide cartap (CP) is non-fluorescent in aqueous solutions. This property makes its determination through direct fluorescent method difficult. In acidic medium and at room temperature, palmatine (PAL) can react with cucurbit[7]uril (CB[7]) to form stable complexes, and the fluorescence intensity of the complex is greatly enhanced. Significant quenching of the fluorescence intensity of the CB[7]-PAL complex was observed with the addition of cartap. Based on the significant quenching of the supramolecular complex fluorescence intensity, a new spectrofluorimetric method with high sensitivity and selectivity was developed to determine cartap in aqueous solution. The fluorescence quenching values (ΔF) showed good linear relationship with cartap concentrations from 0.009 to 2.4 μg mL−1 with a detection limit 0.0029 μg mL−1. The proposed method had been successfully applied to the determination of cartap residues in grain and vegetable with recoveries of 87.4-103%. In addition, the association constants of the complexes formed between the host and the guest were determined. The competing reaction and the supramolecular interaction mechanisms between the cartap and PAL as they fight for occupancy of the CB[7] cavity were studied using spectrofluorimetry, 1H NMR and molecular modeling calculations. The insecticide cartap （CP） is non-fluorescent in aqueous solutions. This property makes its determination through direct fluorescent method difficult. In acidic medium and at room temperature, palmatine （PAL） can react with cucurbit[7]uril （CB[7]） to form stable complexes, and the fluorescence intensity of the complex is greatly enhanced. Significant quenching of the fluorescence intensity of the CB[7]-PAL complex was observed with the addition of cartap. Based on the significant quenching of the supramolecular complex fluorescence intensity, a new spectrofluorimetric method with high sensitivity and selectivity was developed to determine cartap in aqueous solution. The fluorescence quenching values （AF） showed good linear relationship with cartap concentrations from 0.009 to 2.4 ~tg mL-~ with a detection limit 0.0029 ~tg mE-x. The proposed method had been successfully applied to the determination of cartap residues in grain and vegetable with recoveries of 87.4-103%. In addition, the association constants of the complexes formed between the host and the guest were determined. The competing reaction and the supramolecular interaction mechanisms between the cartap and PAL as they fight for occupancy of the CB[7] cavity were studied using spectrofluorimetry, xH NMR and molecular modeling calculations. S1; The insecticide cartap (CP) is non-fluorescent in aqueous solutions.This property makes its determination through direct fluorescent method difficult.In acidic medium and at room temperature,palmatine (PAL) can react with cucurbit[7]uril (CB[7]) to form stable complexes,and the fluorescence intensity of the complex is greatly enhanced.Significant quenching of the fluorescence intensity of the CB[7]-PAL complex was observed with the addition of cartap.Based on the significant quenching of the supramolecular complex fluorescence intensity,a new spectrofluorimetric method with high sensitivity and selectivity was developed to determine cartap in aqueous solution.The fluorescence quenching values (AF) showed good linear relationship with cartap concentrations from 0.009 to 2.4 μg mL-1 with a detection limit 0.0029 μtg mL-1.The proposed method had been successfully applied to the determination of cartap residues in grain and vegetable with recoveries of 87.4-103％.In addition,the association constants of the complexes formed between the host and the guest were determined.The competing reaction and the supramolecular interaction mechanisms between the cartap and PAL as they fight for occupancy of the CB[7] cavity were studied using spectrofluorimetry,1H NMR and molecular modeling calculations. The insecticide cartap (CP) is non-fluorescent in aqueous solutions. This property makes its determination through direct fluorescent method difficult. In acidic medium and at room temperature, palmatine (PAL) can react with cucurbit[7]uril (CB[7]) to form stable complexes, and the fluorescence intensity of the complex is greatly enhanced. Significant quenching of the fluorescence intensity of the CB[7]-PAL complex was observed with the addition of cartap. Based on the significant quenching of the supramolecular complex fluorescence intensity, a new spectrofluorimetric method with high sensitivity and selectivity was developed to determine cartap in aqueous solution. The fluorescence quenching values (DF) showed good linear relationship with cartap concentrations from 0.009 to 2.4 μg mL-1 with a detection limit 0.0029 μg mL-1. The proposed method had been successfully applied to the determination of cartap residues in grain and vegetable with recoveries of 87.4-103%. In addition, the association constants of the complexes formed between the host and the guest were determined. The competing reaction and the supramolecular interaction mechanisms between the cartap and PAL as they fight for occupancy of the CB[7] cavity were studied using spectrofluorimetry, 1H NMR and molecular modeling calculations. |
| Author | JING Xu DU Li-ming WU Hao WU Wen-ying CHANG Yin-xia |
| AuthorAffiliation | Analytical and Testing Center, Shanxi Normal University, Linfen 041000, P.R.China |
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| Cites_doi | 10.1103/PhysRevB.37.785 10.1021/ja055013x 10.1016/S0261-2194(01)00039-4 10.1021/jf101303g 10.1016/S0300-483X(03)00268-3 10.1016/j.talanta.2010.02.066 10.1021/ja993376p 10.1016/j.talanta.2009.10.049 10.1021/jp044369c 10.1021/jp952855h 10.1021/jp075348w 10.1016/j.aquatox.2009.10.006 10.1155/S1110662X05000206 10.1021/jf2019566 10.1021/jf200343b 10.1021/cr960371r 10.1023/B:JIPH.0000011779.65838.44 10.1021/jo00373a018 10.1016/j.saa.2007.07.055 10.1016/j.tox.2005.11.002 10.1021/jo015897c 10.1016/S1671-2927(08)60122-4 10.1021/ja00414a070 10.1021/ar020254k 10.1016/j.jep.2009.08.009 10.1016/j.chroma.2007.09.092 10.1007/BF00710222 10.1039/c1an15078b 10.1016/j.saa.2011.02.045 |
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| DocumentTitleAlternate | Determination of Pesticide Residue Cartap Using a Sensitive Fluorescent Probe |
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| Keywords | cucurbituril palmatine cartap fluorescent probe |
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| Notes | cartap, cucurbit[7]uril, palmatine, fluorescent probe 10-1039/S The insecticide cartap （CP） is non-fluorescent in aqueous solutions. This property makes its determination through direct fluorescent method difficult. In acidic medium and at room temperature, palmatine （PAL） can react with cucurbit[7]uril （CB[7]） to form stable complexes, and the fluorescence intensity of the complex is greatly enhanced. Significant quenching of the fluorescence intensity of the CB[7]-PAL complex was observed with the addition of cartap. Based on the significant quenching of the supramolecular complex fluorescence intensity, a new spectrofluorimetric method with high sensitivity and selectivity was developed to determine cartap in aqueous solution. The fluorescence quenching values （AF） showed good linear relationship with cartap concentrations from 0.009 to 2.4 ~tg mL-~ with a detection limit 0.0029 ~tg mE-x. The proposed method had been successfully applied to the determination of cartap residues in grain and vegetable with recoveries of 87.4-103%. In addition, the association constants of the complexes formed between the host and the guest were determined. The competing reaction and the supramolecular interaction mechanisms between the cartap and PAL as they fight for occupancy of the CB[7] cavity were studied using spectrofluorimetry, xH NMR and molecular modeling calculations. http://dx.doi.org/ http://www.chinaagrisci.com/Jwk_zgnykxen/fileup/PDF/2012,11(11)-1861.pdf |
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| Snippet | The insecticide cartap （CP） is non-fluorescent in aqueous solutions. This property makes its determination through direct fluorescent method difficult. In... The insecticide cartap (CP) is non-fluorescent in aqueous solutions. This property makes its determination through direct fluorescent method difficult. In... S1; The insecticide cartap (CP) is non-fluorescent in aqueous solutions.This property makes its determination through direct fluorescent method difficult.In... |
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| SubjectTerms | cartap cartap cucurbituril palmatine fluorescent probe cucurbituril fluorescent probe palmatine 农药残留 敏感 杀螟丹 相互作用机制 荧光强度 荧光探针 荧光法测定 超分子配合物 |
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| Title | Determination of Pesticide Residue Cartap Using a Sensitive Fluorescent Probe |
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