Determination of Pesticide Residue Cartap Using a Sensitive Fluorescent Probe

The insecticide cartap （CP） is non-fluorescent in aqueous solutions. This property makes its determination through direct fluorescent method difficult. In acidic medium and at room temperature, palmatine （PAL） can react with cucurbit[7]uril （CB[7]） to form stable complexes, and the fluorescence inte...

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Bibliographic Details
Published inJournal of Integrative Agriculture Vol. 11; no. 11; pp. 1861 - 1870
Main Authors JING, Xu, DU, Li-ming, WU, Hao, WU, Wen-ying, CHANG, Yin-xia
Format Journal Article
LanguageEnglish
Published Elsevier B.V 01.11.2012
Science Press
Analytical and Testing Center, Shanxi Normal University, Linfen 041000, P.R.China
KeAi Communications Co., Ltd
Subjects
cartap
cartap cucurbituril palmatine fluorescent probe
cucurbituril
fluorescent probe
palmatine
农药残留
敏感
杀螟丹
相互作用机制
荧光强度
荧光探针
荧光法测定
超分子配合物
cucurbituril
palmatine
cartap
fluorescent probe
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Summary:The insecticide cartap （CP） is non-fluorescent in aqueous solutions. This property makes its determination through direct fluorescent method difficult. In acidic medium and at room temperature, palmatine （PAL） can react with cucurbit[7]uril （CB[7]） to form stable complexes, and the fluorescence intensity of the complex is greatly enhanced. Significant quenching of the fluorescence intensity of the CB[7]-PAL complex was observed with the addition of cartap. Based on the significant quenching of the supramolecular complex fluorescence intensity, a new spectrofluorimetric method with high sensitivity and selectivity was developed to determine cartap in aqueous solution. The fluorescence quenching values （AF） showed good linear relationship with cartap concentrations from 0.009 to 2.4 ~tg mL-~ with a detection limit 0.0029 ~tg mE-x. The proposed method had been successfully applied to the determination of cartap residues in grain and vegetable with recoveries of 87.4-103%. In addition, the association constants of the complexes formed between the host and the guest were determined. The competing reaction and the supramolecular interaction mechanisms between the cartap and PAL as they fight for occupancy of the CB[7] cavity were studied using spectrofluorimetry, xH NMR and molecular modeling calculations.
Bibliography:cartap, cucurbit[7]uril, palmatine, fluorescent probe
10-1039/S
The insecticide cartap （CP） is non-fluorescent in aqueous solutions. This property makes its determination through direct fluorescent method difficult. In acidic medium and at room temperature, palmatine （PAL） can react with cucurbit[7]uril （CB[7]） to form stable complexes, and the fluorescence intensity of the complex is greatly enhanced. Significant quenching of the fluorescence intensity of the CB[7]-PAL complex was observed with the addition of cartap. Based on the significant quenching of the supramolecular complex fluorescence intensity, a new spectrofluorimetric method with high sensitivity and selectivity was developed to determine cartap in aqueous solution. The fluorescence quenching values （AF） showed good linear relationship with cartap concentrations from 0.009 to 2.4 ~tg mL-~ with a detection limit 0.0029 ~tg mE-x. The proposed method had been successfully applied to the determination of cartap residues in grain and vegetable with recoveries of 87.4-103%. In addition, the association constants of the complexes formed between the host and the guest were determined. The competing reaction and the supramolecular interaction mechanisms between the cartap and PAL as they fight for occupancy of the CB[7] cavity were studied using spectrofluorimetry, xH NMR and molecular modeling calculations.
http://dx.doi.org/
http://www.chinaagrisci.com/Jwk_zgnykxen/fileup/PDF/2012,11(11)-1861.pdf
ISSN:2095-3119
2352-3425
DOI:10.1016/S2095-3119(12)60191-9