Development of a nanoparticle-assisted PCR assay for detection of porcine epidemic diarrhea virus

• Published in: Journal of virological methods Vol. 220; pp. 18 - 20
• Main Authors: Yuan, Wanzhe, Li, Yanan, Li, Peng, Song, Qinye, Li, Limin, Sun, Jiguo
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.08.2015
• Subjects: Animals; Coronavirus Infections - diagnosis; Coronavirus Infections - veterinary; Coronavirus Infections - virology; Detection; DNA Primers - genetics; Molecular Diagnostic Techniques - methods; Nanoparticles; nanoPCR; Nucleocapsid Proteins - genetics; Polymerase Chain Reaction - methods; Porcine epidemic diarrhea virus; Porcine epidemic diarrhea virus (PEDV); Porcine epidemic diarrhea virus - genetics; Porcine epidemic diarrhea virus - isolation & purification; RT-PCR; Sensitivity and Specificity; Short Communication; Swine; Swine Diseases - diagnosis; Swine Diseases - virology; Veterinary Medicine - methods; Detection; Porcine epidemic diarrhea virus (PEDV); RT-PCR; nanoPCR

•A nanoPCR method was developed for the detection of PEDV.•The nanoPCR assay was 100-fold more sensitive than a conventional RT-PCR assay.•The lower detection limit was 2.7×10−6ng/μL of PEDV RNA.•This test could be applied for clinical diagnosis and field surveillance of PEDV. Porcine epidemic diarr...