Detergent addition to tryptic digests and ion mobility separation prior to MS/MS improves peptide yield and protein identification for in situ proteomic investigation of frozen and formalin‐fixed paraffin‐embedded adenocarcinoma tissue sections - Djidja, Marie‐Claude; Francese, Simona; Loadman, Paul M.; Sutton, Chris W.; Scriven, Peter; Claude, Emmanuelle; Snel, Marten F.; Franck, Julien; Salzet, Michel; Clench, Malcolm R. (Proteomics (Weinheim), 2009)
Keywords: Adenocarcinoma - chemistry; Analytical, structural and metabolic biochemistry; Animals; Biological and medical sciences; Breast Neoplasms - chemistry; Cell Line, Tumor; Detergents; Disease Models, Animal; Female; Formalin fixed paraffin embedded; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Neoplastic; Humans; Imaging; Life Sciences; MALDI; MCF7; Mice; Mice, Inbred BALB C; Miscellaneous; Neoplasm Proteins - analysis; Neoplasm Proteins - chemistry; Neoplasm Transplantation; Paraffin Embedding; Proteins; Proteomics - methods; Sensitivity and Specificity; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Stress proteins; Trypsin - metabolism; Adenocarcinoma; MCF7; Peptides; In situ; Formalin fixed paraffin embedded; MALDI; Identification; Malignant tumor; Protein; Stress; Imaging; Proteomics; Yield; Stress proteins; Cancer