F11 rs2289252T and rs2036914C Polymorphisms Increase the Activity of Factor XI in Post-trauma Patients with Fractures Despite Thromboprophylaxis

• Published in: Orthopaedic surgery Vol. 8; no. 3; pp. 377 - 382
• Main Authors: Song, Nan, Tian, Ai-xian, Zhang, Jian-min, Jiang, Hong-qiang, Zang, Jia-cheng, Ma, Xin-long
• Format: Journal Article
• Language: English
• Published: Melbourne John Wiley & Sons Australia, Ltd 01.08.2016; John Wiley & Sons, Inc
• Subjects: Adult; Anticoagulants - therapeutic use; Case-Control Studies; Deep vein thrombosis; Factor XI; Factor XI - genetics; Factor XI - metabolism; Female; Fractures; Fractures, Bone - complications; Gene Frequency; Genes; Genetic Markers; Genetic Predisposition to Disease; Genotype; Humans; Male; Middle Aged; Polymorphism; Polymorphism, Single Nucleotide; Risk Factors; Scientific; Trauma; Treatment Failure; Venous Thrombosis - blood; Venous Thrombosis - etiology; Venous Thrombosis - genetics; Venous Thrombosis - prevention & control; Deep vein thrombosis; Factor XI; Fractures; Polymorphism
• ISSN: 1757-7853; 1757-7861
• DOI: 10.1111/os.12262

Objective To evaluate the association between F11 rs2289252, rs2036914 polymorphisms and the activity of clotting factor XI in post‐trauma patients with fractures receiving routine anticoagulation therapy for deep venous thrombosis (DVT). Methods A case‐control study involving 110 consecutive post‐trauma patients with fractures and DVT in our hospital was conducted from April 2014 to October 2015; these patients comprised a DVT group. Another 40 sex‐ and age‐matched patients with fractures but without DVT served as controls. Additionally, 40 sex‐ and age‐matched healthy people were chosen as a normal group. Venous blood samples (2 mL) were drawn from all participants and genomic DNA extracted from the leukocytes of the patients with fracture‐related DVT, whose genotype and allele frequency distribution of F11 gene rs2089252 and rs2036914 single nucleotide polymorphism were then assessed by a sequencing method. The activity of factor XI was measured by a solidification method in all participants, including those in control and normal groups. Results The activity of factor XI in patients with fracture‐related DVT and F11 rs2089252 CT was 1.16 times that of those with CC genotypes (P < 0.0001), whereas in patients with fracture‐related DVT and F11 rs2089252 TT genotypes it was 1.32 times that of those with CC genotypes (P < 0.0001), in patients with fracture‐related DVT and F11 rs2089252 T allele it was 1.24 times that of those with C allele (P < 0.05), in patients with fracture‐related DVT and F11 rs2036914 CC it was 1.35 times that of those with TT genotypes, in patients with fracture‐related DVT and F11 rs2036914 CT genotypes it was 1.12 times that of those with TT genotypes (P < 0.05), and in patients with fracture‐related DVT F11 and rs2036914 C allele it was 1.22 times that of those with T allele (P < 0.05). The activity of factor XI was significantly higher in the control than in the normal group (P < 0.05). Conclusions High activity of factor XI indicates a risk of occurrence of DVT in post‐trauma patients with fractures. F11 rs2089252 and rs2036914 (single nucleotide polymorphisms) are associated with activity of factors XI in such patients despite prophylaxis.