Study on the Prevalence of Pneumocystis jirovecii as a Causative Agent of Lung Pathology in People with Different Immune Status

• Published in: Biomedicines Vol. 11; no. 7; p. 1851
• Main Authors: Harizanov, Rumen, Tsvetkova, Nina, Ivanova, Aleksandra, Enikova, Raina, Videnova, Mihaela, Rainova, Iskra, Kaneva, Eleonora, Kaftandjiev, Iskren, Strashimirov, Dimitar, Yancheva-Petrova, Nina, Simeonovski, Ivan, Levterova, Viktoria, Yanev, Nikolay
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 28.06.2023; MDPI
• Subjects: Acquired immune deficiency syndrome; AIDS; Bronchus; Chronic obstructive pulmonary disease; Coronaviruses; Cysts; Deoxyribonucleic acid; Diagnosis; DNA; Drinking water; Epidemics; genetic techniques; Hematology; HIV; Human immunodeficiency virus; Immune status; immunity; Immunocompetence; Immunocompromised hosts; Infections; Laboratory methods; Lavage; Lung diseases; microscopy; Parasitic diseases; Pathogens; Pneumocystis jirovecii; Pneumocystis pneumonia; Pneumocystosis; Pneumonia; Polymerase chain reaction; Premature babies; Premature birth; Secretions; Software; Sputum; Stains & staining; Sulfamethoxazole; Transplants & implants; Trimethoprim; Trimethoprim-sulfamethoxazole; Netherlands; Bulgaria; United States > US; Germany; genetic techniques; microscopy; immunity; Pneumocystis pneumonia
• DOI: 10.3390/biomedicines11071851
• ISSN: 2227-9059

Background: Pneumocystis pneumonia (PCP) commonly affects immunocompromised individuals, whereas in immunocompetent persons, it occurs relatively rarely, and in most cases, the Pneumocystis infection is detected as an asymptomatic colonization. The present study aimed to establish the prevalence of Pneumocystis jirovecii infection in human hosts with different immune status (immunocompromised and immunocompetent), using molecular diagnostic methods, and to compare their diagnostic value with that of classical staining methods. Methods: We used the collected-to-this-moment data from a prospective study on the prevalence of pneumocystosis among the Bulgarian population. Clinical specimens (including throat secretions, induced sputum, tracheal aspirates, and bronchoalveolar lavage) collected from 220 patients suspected of PCP (153 immunocompetent and 67 immunocompromised patients) were examined with microscopic staining methods and real-time PCR for detection of P. jirovecii. Results: DNA of the pathogen was detected in 38 (17%) specimens (32 immunocompromised patients and 6 immunocompetent subjects). From all 220 clinical samples examined by staining methods, only five (2%) P. jirovecii cysts were detected by the Gomori stain. All patients with PCP were treated with trimethoprim-sulfamethoxazole, but in ten of them (HIV-positive patients), the disease had a fatal outcome. Conclusions: This study is the first in Bulgaria including the main available laboratory methods for diagnosis of human pneumocystosis. Regarding the etiological diagnosis of PCP, in our study the sensitivity of real-time PCR was higher compared to the staining methods. The choice of a method for sample collection and examination has an important role in the efficiency of the laboratory diagnostics.