Sperm processing by swim-up and density gradient is effective in elimination of sperm with DNA damage

Purpose DNA damage may occur during sperm processing, thereby negatively influencing fertilizing ability of the sperm. The present study was designed to compare the effectiveness of gradient and swim-up, either alone or in combination, to eliminate sperm with DNA damage. Methods A total of 51 subjec...

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Published inJournal of assisted reproduction and genetics Vol. 29; no. 6; pp. 557 - 563
Main Authors Jayaraman, Varshini, Upadhya, Dinesh, Narayan, Pratap Kumar, Adiga, Satish Kumar
Format Journal Article
LanguageEnglish
Published Boston Springer US 01.06.2012
Springer Nature B.V
Subjects
Carbon dioxide
Centrifugation, Density Gradient - methods
Centrifuges
Chromatin
Density gradients
DNA Damage
Gynecology
Human Genetics
Humans
In Situ Nick-End Labeling
Infertility
Infertility, Male - pathology
Male
Medicine
Medicine & Public Health
Morphology
Motility
Oligospermia - genetics
Oligozoospermia
Performance evaluation
Pregnancy
Reference Values
Reproductive Medicine
Semen
Semen - cytology
Sperm
Sperm Count
Sperm Motility - genetics
Spermatozoa - cytology
Spermatozoa - physiology
Technical Innovations
United States > US
Sperm selection
Density gradient
Semen preparation
Sperm DNA damage
Swim-up
Online AccessGet full text
ISSN1058-0468
1573-7330
1573-7330
DOI10.1007/s10815-012-9742-x

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Abstract Purpose DNA damage may occur during sperm processing, thereby negatively influencing fertilizing ability of the sperm. The present study was designed to compare the effectiveness of gradient and swim-up, either alone or in combination, to eliminate sperm with DNA damage. Methods A total of 51 subjects visiting the University infertility clinic with normozoospermic parameters, oligozoospermia and teratozoospermia were included. Semen characteristics were analysed by standard criteria; Terminal deoxy nucelotidyl transferase mediated dUTP nick end labeling assay was employed for DNA damage assessment. Results The percentage of TUNEL positive sperm after sperm processing was significantly lower in normozoospermic ( P  < 0.05), oligozoospermic ( P  < 0.001) and teratozoospermic samples ( P  < 0.01). No difference was observed in the incidence of TUNEL positive sperm between the various techniques, suggesting that they are comparable. Conclusions Sperm preparation has been found to result in enrichment of sperm with intact chromatin, which is likely to improve the chances of achieving a viable pregnancy.
AbstractList DNA damage may occur during sperm processing, thereby negatively influencing fertilizing ability of the sperm. The present study was designed to compare the effectiveness of gradient and swim-up, either alone or in combination, to eliminate sperm with DNA damage.PURPOSEDNA damage may occur during sperm processing, thereby negatively influencing fertilizing ability of the sperm. The present study was designed to compare the effectiveness of gradient and swim-up, either alone or in combination, to eliminate sperm with DNA damage.A total of 51 subjects visiting the University infertility clinic with normozoospermic parameters, oligozoospermia and teratozoospermia were included. Semen characteristics were analysed by standard criteria; Terminal deoxy nucelotidyl transferase mediated dUTP nick end labeling assay was employed for DNA damage assessment.METHODSA total of 51 subjects visiting the University infertility clinic with normozoospermic parameters, oligozoospermia and teratozoospermia were included. Semen characteristics were analysed by standard criteria; Terminal deoxy nucelotidyl transferase mediated dUTP nick end labeling assay was employed for DNA damage assessment.The percentage of TUNEL positive sperm after sperm processing was significantly lower in normozoospermic (P < 0.05), oligozoospermic (P < 0.001) and teratozoospermic samples (P < 0.01). No difference was observed in the incidence of TUNEL positive sperm between the various techniques, suggesting that they are comparable.RESULTSThe percentage of TUNEL positive sperm after sperm processing was significantly lower in normozoospermic (P < 0.05), oligozoospermic (P < 0.001) and teratozoospermic samples (P < 0.01). No difference was observed in the incidence of TUNEL positive sperm between the various techniques, suggesting that they are comparable.Sperm preparation has been found to result in enrichment of sperm with intact chromatin, which is likely to improve the chances of achieving a viable pregnancy.CONCLUSIONSSperm preparation has been found to result in enrichment of sperm with intact chromatin, which is likely to improve the chances of achieving a viable pregnancy.
DNA damage may occur during sperm processing, thereby negatively influencing fertilizing ability of the sperm. The present study was designed to compare the effectiveness of gradient and swim-up, either alone or in combination, to eliminate sperm with DNA damage. A total of 51 subjects visiting the University infertility clinic with normozoospermic parameters, oligozoospermia and teratozoospermia were included. Semen characteristics were analysed by standard criteria; Terminal deoxy nucelotidyl transferase mediated dUTP nick end labeling assay was employed for DNA damage assessment. The percentage of TUNEL positive sperm after sperm processing was significantly lower in normozoospermic (P < 0.05), oligozoospermic (P < 0.001) and teratozoospermic samples (P < 0.01). No difference was observed in the incidence of TUNEL positive sperm between the various techniques, suggesting that they are comparable. Sperm preparation has been found to result in enrichment of sperm with intact chromatin, which is likely to improve the chances of achieving a viable pregnancy.
Purpose: DNA damage may occur during sperm processing, thereby negatively influencing fertilizing ability of the sperm. The present study was designed to compare the effectiveness of gradient and swim-up, either alone or in combination, to eliminate sperm with DNA damage. Methods: A total of 51 subjects visiting the University infertility clinic with normozoospermic parameters, oligozoospermia and teratozoospermia were included. Semen characteristics were analysed by standard criteria; Terminal deoxy nucelotidyl transferase mediated dUTP nick end labeling assay was employed for DNA damage assessment. Results: The percentage of TUNEL positive sperm after sperm processing was significantly lower in normozoospermic (P<0.05), oligozoospermic (P<0.001) and teratozoospermic samples (P<0.01). No difference was observed in the incidence of TUNEL positive sperm between the various techniques, suggesting that they are comparable. Conclusions: Sperm preparation has been found to result in enrichment of sperm with intact chromatin, which is likely to improve the chances of achieving a viable pregnancy.
DNA damage may occur during sperm processing, thereby negatively influencing fertilizing ability of the sperm. The present study was designed to compare the effectiveness of gradient and swim-up, either alone or in combination, to eliminate sperm with DNA damage. A total of 51 subjects visiting the University infertility clinic with normozoospermic parameters, oligozoospermia and teratozoospermia were included. Semen characteristics were analysed by standard criteria; Terminal deoxy nucelotidyl transferase mediated dUTP nick end labeling assay was employed for DNA damage assessment. The percentage of TUNEL positive sperm after sperm processing was significantly lower in normozoospermic (P<0.05), oligozoospermic (P<0.001) and teratozoospermic samples (P<0.01). No difference was observed in the incidence of TUNEL positive sperm between the various techniques, suggesting that they are comparable. Sperm preparation has been found to result in enrichment of sperm with intact chromatin, which is likely to improve the chances of achieving a viable pregnancy.[PUBLICATION ABSTRACT]
Purpose DNA damage may occur during sperm processing, thereby negatively influencing fertilizing ability of the sperm. The present study was designed to compare the effectiveness of gradient and swim-up, either alone or in combination, to eliminate sperm with DNA damage. Methods A total of 51 subjects visiting the University infertility clinic with normozoospermic parameters, oligozoospermia and teratozoospermia were included. Semen characteristics were analysed by standard criteria; Terminal deoxy nucelotidyl transferase mediated dUTP nick end labeling assay was employed for DNA damage assessment. Results The percentage of TUNEL positive sperm after sperm processing was significantly lower in normozoospermic ( P  < 0.05), oligozoospermic ( P  < 0.001) and teratozoospermic samples ( P  < 0.01). No difference was observed in the incidence of TUNEL positive sperm between the various techniques, suggesting that they are comparable. Conclusions Sperm preparation has been found to result in enrichment of sperm with intact chromatin, which is likely to improve the chances of achieving a viable pregnancy.
Author Narayan, Pratap Kumar
Adiga, Satish Kumar
Upadhya, Dinesh
Jayaraman, Varshini
Author_xml – sequence: 1
  givenname: Varshini
  surname: Jayaraman
  fullname: Jayaraman, Varshini
  email: varsh1981@gmail.com
  organization: Division of Reproductive Medicine, Clinical Embryology Laboratory, Kasturba Medical College Manipal University
– sequence: 2
  givenname: Dinesh
  surname: Upadhya
  fullname: Upadhya, Dinesh
  organization: Division of Reproductive Medicine, Clinical Embryology Laboratory, Kasturba Medical College Manipal University
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  givenname: Pratap Kumar
  surname: Narayan
  fullname: Narayan, Pratap Kumar
  organization: Division of Reproductive Medicine, Clinical Embryology Laboratory, Kasturba Medical College Manipal University
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  givenname: Satish Kumar
  surname: Adiga
  fullname: Adiga, Satish Kumar
  organization: Division of Reproductive Medicine, Clinical Embryology Laboratory, Kasturba Medical College Manipal University
BackLink https://www.ncbi.nlm.nih.gov/pubmed/22411295$$D View this record in MEDLINE/PubMed
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1573-7330
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Issue 6
Keywords Sperm selection
Density gradient
Semen preparation
Sperm DNA damage
Swim-up
Language English
License http://www.springer.com/tdm
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OpenAccessLink https://link.springer.com/content/pdf/10.1007/s10815-012-9742-x.pdf
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PublicationDate 2012-06-01
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  day: 01
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PublicationSubtitle Official Publication of ALPHA, Scientists in Reproductive Medicine
PublicationTitle Journal of assisted reproduction and genetics
PublicationTitleAbbrev J Assist Reprod Genet
PublicationTitleAlternate J Assist Reprod Genet
PublicationYear 2012
Publisher Springer US
Springer Nature B.V
Publisher_xml – name: Springer US
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Snippet Purpose DNA damage may occur during sperm processing, thereby negatively influencing fertilizing ability of the sperm. The present study was designed to...
DNA damage may occur during sperm processing, thereby negatively influencing fertilizing ability of the sperm. The present study was designed to compare the...
Purpose: DNA damage may occur during sperm processing, thereby negatively influencing fertilizing ability of the sperm. The present study was designed to...
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springer
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StartPage 557
SubjectTerms Carbon dioxide
Centrifugation, Density Gradient - methods
Centrifuges
Chromatin
Density gradients
DNA Damage
Gynecology
Human Genetics
Humans
In Situ Nick-End Labeling
Infertility
Infertility, Male - pathology
Male
Medicine
Medicine & Public Health
Morphology
Motility
Oligospermia - genetics
Oligozoospermia
Performance evaluation
Pregnancy
Reference Values
Reproductive Medicine
Semen
Semen - cytology
Sperm
Sperm Count
Sperm Motility - genetics
Spermatozoa - cytology
Spermatozoa - physiology
Technical Innovations
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Title Sperm processing by swim-up and density gradient is effective in elimination of sperm with DNA damage
URI https://link.springer.com/article/10.1007/s10815-012-9742-x
https://www.ncbi.nlm.nih.gov/pubmed/22411295
https://www.proquest.com/docview/1019086117
https://www.proquest.com/docview/1019615682
https://www.proquest.com/docview/1093468563
https://pubmed.ncbi.nlm.nih.gov/PMC3370043
Volume 29
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