Eutopic and ectopic stromal cells from patients with endometriosis exhibit differential invasive, adhesive, and proliferative behavior

• Published in: Fertility and sterility Vol. 100; no. 3; pp. 761 - 769
• Main Authors: Delbandi, Ali-Akbar, M.Sc, Mahmoudi, Mahmoud, Ph.D, Shervin, Adel, M.D, Akbari, Elham, M.D, Jeddi-Tehrani, Mahmood, Ph.D, Sankian, Mojtaba, Ph.D, Kazemnejad, Somayeh, Ph.D, Zarnani, Amir-Hassan, Ph.D
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.09.2013
• Subjects: adhesion; Adult; animal ovaries; Cell Adhesion; Cell Movement; Cell Proliferation; Cell- och molekylärbiologi; Cells, Cultured; Choristoma - pathology; cytokine; Cytokines - metabolism; Endometrioma; Endometriosis - pathology; Endometrium; eutopic; extracellular matrix; Female; Humans; immunophenotype; in vitro studies; interleukin-8; Internal Medicine; invasion; Klinisk medicin; Medicin och hälsovetenskap; Medicinska och farmaceutiska grundvetenskaper; menstruation; Middle Aged; Obstetrics and Gynecology; pathogenesis; patients; proliferation; Reproduktionsmedicin och gynekologi; stromal cells; Stromal Cells - pathology; Young Adult; cytokine; invasion; proliferation; Endometrioma; immunophenotype; adhesion; eutopic
• ISSN: 0015-0282; 1556-5653; 1556-5653
• DOI: 10.1016/j.fertnstert.2013.04.041

Objective To study immunophenotype, differential proliferation capacity, invasiveness, adhesion, and cytokine production in ectopic and eutopic endometrial stromal cells (EESCs and EuESCs) from patients with endometriosis. Design In vitro study. Setting Academic research center. Patient(s) Patients with ovarian endometriosis (endometrioma) and nonendometriotic controls. Intervention(s) None. Main Outcome Measure(s) EESCs and EuESCs from 25 patients with endometrioma and ESCs from 20 nonendometriotic controls (CESCs) were isolated, and their immunophenotype, proliferation, invasion, adhesion, and cytokine production were assessed and compared. Result(s) Isolated ESCs from all three sources expressed markers specific for cells of mesenchymal origin but were negative for hematopoietic markers. EESCs exhibited a significantly lower proliferation rate in fibronectin-coated plates and less invasive capacity compared with CESCs or EuESCs. Among all stromal cell groups studied, EuESCs showed the highest invasive behavior. EESCs adhered more firmly to extracellular matrix than EuESCs or CESCs in all time intervals examined. The levels of interleukin (IL) -6 and IL-8 production by EESCs were significantly higher compared with those of EuESCs or CESCs. Conclusion(s) The results of the present study demonstrated that retrograde menstruation alone does not account for the pathogenesis of endometriosis as eutopic and ectopic counterparts of ESCs from patients with endometriosis exhibit differential invasive, adhesive, and proliferative behavior.