Effect of ionic strength, pH and polymer concentration on the separation of DNA fragments in the presence of electroosmotic flow

• Published in: Journal of Chromatography A Vol. 894; no. 1; pp. 219 - 230
• Main Authors: Tseng, Wei-Lung, Hsieh, Ming-Mu, Wang, Shang-Ji, Chang, Huan-Tsung
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Amsterdam Elsevier B.V 13.10.2000; Elsevier
• Subjects: Analytical, structural and metabolic biochemistry; Biological and medical sciences; Buffers; DNA; DNA - isolation & purification; Dna, deoxyribonucleoproteins; Electrophoresis, Capillary - methods; Fundamental and applied biological sciences. Psychology; Hydrogen-Ion Concentration; Nucleic acids; Osmolar Concentration; Osmosis; Poly(ethylene oxide); Polymers; Electroosmotic flow; Poly(ethylene oxide); Buffer composition; pH effects; Ionic strength; DNA; Ethylene oxide polymer; Separation method; Zone electrophoresis; Phase composition; Capillary electrophoresis; Electroosmosis; pH; Mobile phase

DNA separations in the presence of electroosmotic flow (EOF) using poly(ethylene oxide) (PEO) solutions have been demonstrated. During the separations, PEO entered capillaries filled with Tris–borate (TB) free buffers by EOF and acted as sieving matrices. We have found that ionic strength and pH of...