Solid-state fermentation of oil palm frond petiole for lignin peroxidase and xylanase-rich cocktail production
In current practice, oil palm frond leaflets and stems are re-used for soil nutrient recycling, while the petioles are typically burned. Frond petioles have high commercialization value, attributed to high lignocellulose fiber content and abundant of juice containing free reducing sugars. Pressed pe...
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Published in | 3 Biotech Vol. 8; no. 5; pp. 259 - 11 |
---|---|
Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Berlin/Heidelberg
Springer Berlin Heidelberg
01.05.2018
Springer Nature B.V |
Subjects | |
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Abstract | In current practice, oil palm frond leaflets and stems are re-used for soil nutrient recycling, while the petioles are typically burned. Frond petioles have high commercialization value, attributed to high lignocellulose fiber content and abundant of juice containing free reducing sugars. Pressed petiole fiber is the subject of interest in this study for the production of lignocellulolytic enzyme. The initial characterization showed the combination of 0.125 mm frond particle size and 60% moisture content provided a surface area of 42.3 m
2
/g, porosity of 12.8%, and density of 1.2 g/cm
3
, which facilitated fungal solid-state fermentation. Among the several species of
Aspergillus
and
Trichoderma
tested,
Aspergillus awamori
MMS4 yielded the highest xylanase (109 IU/g) and cellulase (12 IU/g), while
Trichoderma virens
UKM1 yielded the highest lignin peroxidase (222 IU/g). Crude enzyme cocktail also contained various sugar residues, mainly glucose and xylose (0.1–0.4 g/L), from the hydrolysis of cellulose and hemicellulose. FT-IR analysis of the fermented petioles observed reduction in cellulose crystallinity (
I
900/1098
), cellulose–lignin (
I
900/1511
), and lignin–hemicellulose (
I
1511/1738
) linkages. The study demonstrated successful bioconversion of chemically untreated frond petioles into lignin peroxidase and xylanase-rich enzyme cocktail under SSF condition. |
---|---|
AbstractList | In current practice, oil palm frond leaflets and stems are re-used for soil nutrient recycling, while the petioles are typically burned. Frond petioles have high commercialization value, attributed to high lignocellulose fiber content and abundant of juice containing free reducing sugars. Pressed petiole fiber is the subject of interest in this study for the production of lignocellulolytic enzyme. The initial characterization showed the combination of 0.125 mm frond particle size and 60% moisture content provided a surface area of 42.3 m
/g, porosity of 12.8%, and density of 1.2 g/cm
, which facilitated fungal solid-state fermentation. Among the several species of
and
tested,
MMS4 yielded the highest xylanase (109 IU/g) and cellulase (12 IU/g), while
UKM1 yielded the highest lignin peroxidase (222 IU/g). Crude enzyme cocktail also contained various sugar residues, mainly glucose and xylose (0.1-0.4 g/L), from the hydrolysis of cellulose and hemicellulose. FT-IR analysis of the fermented petioles observed reduction in cellulose crystallinity (
), cellulose-lignin (
), and lignin-hemicellulose (
) linkages. The study demonstrated successful bioconversion of chemically untreated frond petioles into lignin peroxidase and xylanase-rich enzyme cocktail under SSF condition. In current practice, oil palm frond leaflets and stems are re-used for soil nutrient recycling, while the petioles are typically burned. Frond petioles have high commercialization value, attributed to high lignocellulose fiber content and abundant of juice containing free reducing sugars. Pressed petiole fiber is the subject of interest in this study for the production of lignocellulolytic enzyme. The initial characterization showed the combination of 0.125 mm frond particle size and 60% moisture content provided a surface area of 42.3 m²/g, porosity of 12.8%, and density of 1.2 g/cm³, which facilitated fungal solid-state fermentation. Among the several species of Aspergillus and Trichoderma tested, Aspergillus awamori MMS4 yielded the highest xylanase (109 IU/g) and cellulase (12 IU/g), while Trichoderma virens UKM1 yielded the highest lignin peroxidase (222 IU/g). Crude enzyme cocktail also contained various sugar residues, mainly glucose and xylose (0.1–0.4 g/L), from the hydrolysis of cellulose and hemicellulose. FT-IR analysis of the fermented petioles observed reduction in cellulose crystallinity (I₉₀₀/₁₀₉₈), cellulose–lignin (I₉₀₀/₁₅₁₁), and lignin–hemicellulose (I₁₅₁₁/₁₇₃₈) linkages. The study demonstrated successful bioconversion of chemically untreated frond petioles into lignin peroxidase and xylanase-rich enzyme cocktail under SSF condition. In current practice, oil palm frond leaflets and stems are re-used for soil nutrient recycling, while the petioles are typically burned. Frond petioles have high commercialization value, attributed to high lignocellulose fiber content and abundant of juice containing free reducing sugars. Pressed petiole fiber is the subject of interest in this study for the production of lignocellulolytic enzyme. The initial characterization showed the combination of 0.125 mm frond particle size and 60% moisture content provided a surface area of 42.3 m 2 /g, porosity of 12.8%, and density of 1.2 g/cm 3 , which facilitated fungal solid-state fermentation. Among the several species of Aspergillus and Trichoderma tested, Aspergillus awamori MMS4 yielded the highest xylanase (109 IU/g) and cellulase (12 IU/g), while Trichoderma virens UKM1 yielded the highest lignin peroxidase (222 IU/g). Crude enzyme cocktail also contained various sugar residues, mainly glucose and xylose (0.1–0.4 g/L), from the hydrolysis of cellulose and hemicellulose. FT-IR analysis of the fermented petioles observed reduction in cellulose crystallinity ( I 900/1098 ), cellulose–lignin ( I 900/1511 ), and lignin–hemicellulose ( I 1511/1738 ) linkages. The study demonstrated successful bioconversion of chemically untreated frond petioles into lignin peroxidase and xylanase-rich enzyme cocktail under SSF condition. In current practice, oil palm frond leaflets and stems are re-used for soil nutrient recycling, while the petioles are typically burned. Frond petioles have high commercialization value, attributed to high lignocellulose fiber content and abundant of juice containing free reducing sugars. Pressed petiole fiber is the subject of interest in this study for the production of lignocellulolytic enzyme. The initial characterization showed the combination of 0.125 mm frond particle size and 60% moisture content provided a surface area of 42.3 m2/g, porosity of 12.8%, and density of 1.2 g/cm3, which facilitated fungal solid-state fermentation. Among the several species of Aspergillus and Trichoderma tested, Aspergillus awamori MMS4 yielded the highest xylanase (109 IU/g) and cellulase (12 IU/g), while Trichoderma virens UKM1 yielded the highest lignin peroxidase (222 IU/g). Crude enzyme cocktail also contained various sugar residues, mainly glucose and xylose (0.1–0.4 g/L), from the hydrolysis of cellulose and hemicellulose. FT-IR analysis of the fermented petioles observed reduction in cellulose crystallinity (I900/1098), cellulose–lignin (I900/1511), and lignin–hemicellulose (I1511/1738) linkages. The study demonstrated successful bioconversion of chemically untreated frond petioles into lignin peroxidase and xylanase-rich enzyme cocktail under SSF condition. In current practice, oil palm frond leaflets and stems are re-used for soil nutrient recycling, while the petioles are typically burned. Frond petioles have high commercialization value, attributed to high lignocellulose fiber content and abundant of juice containing free reducing sugars. Pressed petiole fiber is the subject of interest in this study for the production of lignocellulolytic enzyme. The initial characterization showed the combination of 0.125 mm frond particle size and 60% moisture content provided a surface area of 42.3 m2/g, porosity of 12.8%, and density of 1.2 g/cm3, which facilitated fungal solid-state fermentation. Among the several species of Aspergillus and Trichoderma tested, Aspergillus awamori MMS4 yielded the highest xylanase (109 IU/g) and cellulase (12 IU/g), while Trichoderma virens UKM1 yielded the highest lignin peroxidase (222 IU/g). Crude enzyme cocktail also contained various sugar residues, mainly glucose and xylose (0.1-0.4 g/L), from the hydrolysis of cellulose and hemicellulose. FT-IR analysis of the fermented petioles observed reduction in cellulose crystallinity (I900/1098), cellulose-lignin (I900/1511), and lignin-hemicellulose (I1511/1738) linkages. The study demonstrated successful bioconversion of chemically untreated frond petioles into lignin peroxidase and xylanase-rich enzyme cocktail under SSF condition.In current practice, oil palm frond leaflets and stems are re-used for soil nutrient recycling, while the petioles are typically burned. Frond petioles have high commercialization value, attributed to high lignocellulose fiber content and abundant of juice containing free reducing sugars. Pressed petiole fiber is the subject of interest in this study for the production of lignocellulolytic enzyme. The initial characterization showed the combination of 0.125 mm frond particle size and 60% moisture content provided a surface area of 42.3 m2/g, porosity of 12.8%, and density of 1.2 g/cm3, which facilitated fungal solid-state fermentation. Among the several species of Aspergillus and Trichoderma tested, Aspergillus awamori MMS4 yielded the highest xylanase (109 IU/g) and cellulase (12 IU/g), while Trichoderma virens UKM1 yielded the highest lignin peroxidase (222 IU/g). Crude enzyme cocktail also contained various sugar residues, mainly glucose and xylose (0.1-0.4 g/L), from the hydrolysis of cellulose and hemicellulose. FT-IR analysis of the fermented petioles observed reduction in cellulose crystallinity (I900/1098), cellulose-lignin (I900/1511), and lignin-hemicellulose (I1511/1738) linkages. The study demonstrated successful bioconversion of chemically untreated frond petioles into lignin peroxidase and xylanase-rich enzyme cocktail under SSF condition. |
ArticleNumber | 259 |
Author | Mohamad Ikubar, Mohamed Roslan Md. Salleh, Madihah Yahya, Adibah Abdul Manan, Musaalbakri |
Author_xml | – sequence: 1 givenname: Mohamed Roslan surname: Mohamad Ikubar fullname: Mohamad Ikubar, Mohamed Roslan organization: Biorefinery Technology Laboratory, Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia – sequence: 2 givenname: Musaalbakri surname: Abdul Manan fullname: Abdul Manan, Musaalbakri organization: Biotechnology and Nanotechnology Research Centre, Malaysian Agricultural Research and Development Institute (MARDI) – sequence: 3 givenname: Madihah surname: Md. Salleh fullname: Md. Salleh, Madihah organization: Biorefinery Technology Laboratory, Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia – sequence: 4 givenname: Adibah surname: Yahya fullname: Yahya, Adibah email: adibah@fbb.utm.my organization: Biorefinery Technology Laboratory, Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/29765817$$D View this record in MEDLINE/PubMed |
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Keywords | Oil palm fronds Solid-state fermentation sp s sp Lignocellulolytic enzymes Trichoderma sp Aspergillus sp |
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Snippet | In current practice, oil palm frond leaflets and stems are re-used for soil nutrient recycling, while the petioles are typically burned. Frond petioles have... |
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SubjectTerms | Agriculture Aspergillus awamori Bioconversion biogeochemical cycles Bioinformatics Biomaterials Biotechnology biotransformation Cancer Research Cellulase Cellulose Chemistry Chemistry and Materials Science Commercialization crystal structure Elaeis guineensis endo-1,4-beta-glucanase Enzymes Fermentation Fermented food fiber content Fourier transform infrared spectroscopy Fungi glucose Hemicellulose hydrolysis Infrared radiation juices Lignin Lignin peroxidase Lignocellulose Moisture content Oil palm trees Original Original Article particle size Peroxidase petioles Porosity reducing sugars Soil moisture soil nutrients Soil porosity Solid state Solid state fermentation Stem Cells stems Substrates Sugar surface area Trichoderma virens Water content Xylanase xylanases Xylose |
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Title | Solid-state fermentation of oil palm frond petiole for lignin peroxidase and xylanase-rich cocktail production |
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