Tissue-specific mRNA Expression Profiles of Human Nuclear Receptor Subfamilies

• Published in: DRUG METABOLISM AND PHARMACOKINETICS Vol. 19; no. 2; pp. 135 - 149
• Main Authors: Nishimura, Masuhiro, Naito, Shinsaku, Yokoi, Tsuyoshi
• Format: Journal Article
• Language: English; Japanese
• Published: England Elsevier Ltd 2004; Japanese Society for the Study of Xenobiotics
• Subjects: Adolescent; Adult; Aged; Aryl Hydrocarbon Hydroxylases - metabolism; ATP-Binding Cassette Transporters - metabolism; Cell Nucleus - metabolism; Cytochrome P-450 CYP3A; DNA Primers; Female; Humans; Male; Middle Aged; Molecular Sequence Data; mRNA expression; nuclear receptor; Oligonucleotides - metabolism; Organ Specificity; Oxidoreductases, N-Demethylating - metabolism; Pregnancy; quantification; Receptors, Cytoplasmic and Nuclear - metabolism; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - biosynthesis; RNA, Messenger - genetics; Tissue Distribution; nuclear receptor; quantification; mRNA expression; tissue distribution

Pairs of forward and reverse primers and TaqMan probes specific to each human nuclear receptor were prepared. Analysis of the mRNA expression level of each target of 43 nuclear receptors in total RNA from single and pooled specimens of various human organs (liver, kidney, adrenal gland, lung, heart,...