ALK detection in lung cancer: identification of atypical and cryptic ALK rearrangements using an optimal algorithm

• Published in: Journal of cancer research and clinical oncology Vol. 146; no. 5; pp. 1307 - 1320
• Main Authors: Liu, Yuanyuan, Wu, Shafei, Shi, Xiaohua, Liang, Zhiyong, Zeng, Xuan
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.05.2020; Springer Nature B.V
• Subjects: Adult; Aged; Aged, 80 and over; Algorithms; Anaplastic Lymphoma Kinase - genetics; Cancer Research; Carcinoma, Non-Small-Cell Lung - enzymology; Carcinoma, Non-Small-Cell Lung - genetics; Chromosome 2; Female; Gene Fusion; Gene Rearrangement; Genomics; Hematology; High-Throughput Nucleotide Sequencing; Humans; Immunohistochemistry; In Situ Hybridization, Fluorescence; Internal Medicine; Lung cancer; Lung Neoplasms - enzymology; Lung Neoplasms - genetics; Male; Medicine; Medicine & Public Health; Middle Aged; Non-small cell lung carcinoma; Oncology; Original Article – Clinical Oncology; Original – Clinical Oncology; Retrospective Studies; Small cell lung carcinoma; Atypical; False negative; Anaplastic lymphoma kinase; Non-small cell lung cancer; Rearrangement; Cryptic

Purpose IHC, FISH, and NGS are common methods of ALK evaluation in NSCLC. The purpose of this study was to investigate whether ALK false positives or false negatives occurred more often in daily routines. An approach to identify ALK fusion was then proposed. Materials and methods We analyzed 1815 ca...