Cross‐species hybridization of a Borrelia burgdorferi DNA array reveals infection‐ and culture‐associated genes of the unsequenced genome of the relapsing fever agent Borrelia hermsii

• Published in: Molecular microbiology Vol. 51; no. 3; pp. 729 - 748
• Main Authors: Zhong, Jianmin, Barbour, Alan G.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Science Ltd 01.02.2004; Blackwell Science; Blackwell Publishing Ltd
• Subjects: Animals; Bacterial diseases; Bacteriology; Biological and medical sciences; Borrelia - genetics; Borrelia burgdorferi; Borrelia burgdorferi - genetics; Borrelia hermsii; Borrelia infections; Borrelia Infections - microbiology; DNA arrays; DNA, Bacterial - genetics; Fundamental and applied biological sciences. Psychology; Gene Expression Profiling; Gene Expression Regulation, Bacterial; Genome, Bacterial; Human bacterial diseases; Infectious diseases; Medical sciences; Mice; Mice, Inbred BALB C; Microbiology; Miscellaneous; Molecular Sequence Data; Nucleic Acid Hybridization; Oligonucleotide Array Sequence Analysis; Open Reading Frames; Plasmids - genetics; Regression Analysis; Relapsing Fever - microbiology; Sensitivity and Specificity; Species Specificity; Tropical bacterial diseases; Borrelia burgdorferi; Spirochaetaceae; Spirochaetales; Microbiology; Borrelia infection; Infection; Gene; Spirochaetosis; Bacteriosis; Bacteria; Relapsing fever; Genome; Borrelia hermsii

Summary The known genome sequence of Borrelia burgdorferi, an agent of Lyme borreliosis, was used to study the genetic content and gene expression in B. hermsii, another spirochete pathogen and a cause of relapsing fever. Cross‐species hybridization of a DNA array representing 1628 open reading fram...