Physico-chemical properties of the encapsulation matrix and germination of carrot somatic embryos

Carrot somatic embryos were encapsulated in alginate gel beads. To improve the quality of a "synthetic seed" coating, the rheology and dehydration properties of different matrices were tested. By increasing alginate and CaCl2 concentrations, additional mineral elements were shown to increa...

Full description

Saved in:
Bibliographic Details
Published inBiotechnology and bioengineering Vol. 46; no. 6; p. 573
Main Authors Timbert, R. (Universite de Technologie de Compiegne, Compiegne, France.), Barbotin, J.N, Kersulec, A, Bazinet, C, Thomas, D
Format Journal Article
LanguageEnglish
Published United States 20.06.1995
Subjects
CULTIVO IN VITRO
CULTURE IN VITRO
DAUCUS CAROTA
Online AccessGet more information

Cover

More Information
Summary:Carrot somatic embryos were encapsulated in alginate gel beads. To improve the quality of a "synthetic seed" coating, the rheology and dehydration properties of different matrices were tested. By increasing alginate and CaCl2 concentrations, additional mineral elements were shown to increase resistance to rupture, and to depress the germination of somatic embryos. A polysaccharide addition was found to slow the alginate matrix dehydration; alginate-gellan gum and alginate-kaolin matrices could preserve the viability of somatic embryos at low relative humidities (30% to 35% germinations at 50% relative humidity) to a greater extent than other matrices
Bibliography:F60
9718244
ISSN:0006-3592
1097-0290
DOI:10.1002/bit.260460610