Comprehensive screening of industrially relevant components at genome scale using a high-quality gene overexpression collection of Corynebacterium glutamicum

• Published in: Trends in biotechnology (Regular ed.) Vol. 43; no. 1; pp. 220 - 247
• Main Authors: Liu, Jiao, Zhao, Xiaojia, Cheng, Haijiao, Guo, Yanmei, Ni, Xiaomeng, Wang, Lixian, Sun, Guannan, Wen, Xiao, Chen, Jiuzhou, Wang, Jin, An, Jingjing, Guo, Xuan, Shi, Zhenkun, Li, Haoran, Wang, Ruoyu, Zhao, Muqiang, Liao, Xiaoping, Wang, Yu, Zheng, Ping, Wang, Meng, Sun, Jibin
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.01.2025; Elsevier Limited
• Subjects: amino acid biomanufacturing; Amino acids; Bacteria; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Biotechnology; Capillary electrophoresis; Carbohydrates; Cloning; Collection; Controllability; Corynebacterium glutamicum; Corynebacterium glutamicum - genetics; Corynebacterium glutamicum - metabolism; DNA methylation; DNA repair; E coli; Enzymes; Escherichia coli - genetics; Escherichia coli - metabolism; functional components; Gene amplification; Gene expression; Gene Expression Regulation, Bacterial - genetics; gene overexpression collection; Genetic engineering; Genome, Bacterial - genetics; Genomes; hyperosmotic tolerance; Industrial Microbiology - methods; Integrated circuits; Internal Medicine; Interrogation; Lysine; Metabolic Engineering - methods; Microorganisms; Mutation; Plasmids; Proteins; Screening; Transcription factors; amino acid biomanufacturing; functional components; Corynebacterium glutamicum; hyperosmotic tolerance; gene overexpression collection

A high-quality gene overexpression collection of Corynebacterium glutamicum was constructed with a highly automated biofoundry.Functional components for enhanced stress tolerance and amino acid transport were comprehensively screened at a genome scale.Transcriptional factors regulating cell division...