Activation Dynamics and Immunoglobulin Evolution of Pre-existing and Newly Generated Human Memory B cell Responses to Influenza Hemagglutinin

• Published in: Immunity (Cambridge, Mass.) Vol. 51; no. 2; pp. 398 - 410.e5
• Main Authors: Andrews, Sarah F., Chambers, Michael J., Schramm, Chaim A., Plyler, Jason, Raab, Julie E., Kanekiyo, Masaru, Gillespie, Rebecca A., Ransier, Amy, Darko, Sam, Hu, Jianfei, Chen, Xuejun, Yassine, Hadi M., Boyington, Jeffrey C., Crank, Michelle C., Chen, Grace L., Coates, Emily, Mascola, John R., Douek, Daniel C., Graham, Barney S., Ledgerwood, Julie E., McDermott, Adrian B.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 20.08.2019; Elsevier Limited
• Subjects: activated B cells; Adult; Antibodies, Viral - metabolism; Antibody Formation; Antigens; B-Lymphocyte Subsets - immunology; B-Lymphocytes - immunology; Binding sites; Biosensors; Cell activation; Cells, Cultured; Clinical trials; Epitopes; Epitopes - immunology; Evolution; Female; Flow cytometry; H7N9; hemagglutinin; Hemagglutinin Glycoproteins, Influenza Virus - immunology; Hemagglutinins; Human behavior; Humans; Ig repertoire; Immunity; Immunoglobulins; Immunologic Memory; Immunological memory; Influenza; Influenza A; Influenza A Virus, H7N9 Subtype - physiology; Influenza Vaccines - immunology; Influenza, Human - immunology; Kinetics; Lymphocyte Activation; Lymphocytes B; Male; memory B cells; Memory cells; Middle Aged; Pandemics; Phenotype; Phenotypes; Receptors, Antigen, B-Cell - genetics; Single-Cell Analysis; Statistical analysis; Tbet; Vaccination; vaccine response; Vaccines; Viruses; Young Adult; influenza; Ig repertoire; hemagglutinin; activated B cells; Tbet; H7N9; memory B cells; vaccine response

Vaccine-induced memory B cell responses to evolving viruses like influenza A involve activation of pre-existing immunity and generation of new responses. To define the contribution of these two types of responses, we analyzed the response to H7N9 vaccination in H7N9-naive adults. We performed compre...