Effect of sympathetic denervation on the rate of protein synthesis in rat skeletal muscle

Department of Physiology, Biochemistry and Immunology, School of Medicine, University of São Paulo, 14049-900 Ribeirão Preto, São Paulo, Brazil Submitted 18 August 2003 ; accepted in final form 28 November 2003 Rates of protein synthesis were investigated in skeletal muscles from rats submitted to c...

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Published inAmerican journal of physiology: endocrinology and metabolism Vol. 286; no. 4; pp. E642 - E647
Main Authors Navegantes, Luiz Carlos C, Resano, Neusa M. Z, Baviera, Amanda M, Migliorini, Renato H, Kettelhut, Isis C
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Abstract Department of Physiology, Biochemistry and Immunology, School of Medicine, University of São Paulo, 14049-900 Ribeirão Preto, São Paulo, Brazil Submitted 18 August 2003 ; accepted in final form 28 November 2003 Rates of protein synthesis were investigated in skeletal muscles from rats submitted to chemical and surgical sympathectomy. Three models of sympathetic denervation were used: 1 ) treatment with guanethidine (100 mg·kg -1 ·day -1 sc); 2 ) lumbar sympathetic denervation (surgical excision of the second and third lumbar ganglia of the sympathetic chain, from which arises the postganglionic fibers to the skeletal muscles of rat hindlimb); and 3 ) adrenodemedullation. Protein synthesis was estimated in isolated soleus muscle by the rate of incorporation of [ 14 C]tyrosine (0.1 mM, 0.05 µCi/ml) into total protein. Soleus isolated after 2 and 4 days of chemical sympathectomy or after 3 days of lumbar denervation showed a 17-20% statistically significant decrease in in vitro rates of protein synthesis. These effects were reverted by addition of 10 -5 M isoproterenol or epinephrine in vitro. Neither clenbuterol nor isoproterenol (10 -7 , 10 -6 , or 10 -5 M) in vitro affected the rate of protein synthesis in soleus from normal rats. On the other hand, clenbuterol or epinephrine (10 -5 M) increased by 20% the rate of protein synthesis in soleus muscles from adrenodemedullated rats and prevented its decrease in muscles from fasted rats. The data suggest that the sympathetic nervous system stimulates protein synthesis in oxidative muscles, probably through the activation of 2 -adrenoceptors, especially in situations of hormonal or nutritional deficiency. catecholamines; isoproterenol; clenbuterol; surgical and chemical sympathectomy Address for reprint requests and other correspondence: I. C. Kettelhut, Dept. of Biochemistry, School of Medicine, USP, 14049-900 Ribeirão Preto, SP, Brazil (E-mail: idckette{at}fmrp.usp.br ).
AbstractList Rates of protein synthesis were investigated in skeletal muscles from rats submitted to chemical and surgical sympathectomy. Three models of sympathetic denervation were used: 1) treatment with guanethidine (100 mg·kg -1 ·day -1 sc); 2) lumbar sympathetic denervation (surgical excision of the second and third lumbar ganglia of the sympathetic chain, from which arises the postganglionic fibers to the skeletal muscles of rat hindlimb); and 3) adrenodemedullation. Protein synthesis was estimated in isolated soleus muscle by the rate of incorporation of [ 14 C]tyrosine (0.1 mM, 0.05 μCi/ml) into total protein. Soleus isolated after 2 and 4 days of chemical sympathectomy or after 3 days of lumbar denervation showed a 17-20% statistically significant decrease in in vitro rates of protein synthesis. These effects were reverted by addition of 10 -5 M isoproterenol or epinephrine in vitro. Neither clenbuterol nor isoproterenol (10 -7 , 10 -6 , or 10 -5 M) in vitro affected the rate of protein synthesis in soleus from normal rats. On the other hand, clenbuterol or epinephrine (10 -5 M) increased by 20% the rate of protein synthesis in soleus muscles from adrenodemedullated rats and prevented its decrease in muscles from fasted rats. The data suggest that the sympathetic nervous system stimulates protein synthesis in oxidative muscles, probably through the activation of β 2 -adrenoceptors, especially in situations of hormonal or nutritional deficiency.
Department of Physiology, Biochemistry and Immunology, School of Medicine, University of São Paulo, 14049-900 Ribeirão Preto, São Paulo, Brazil Submitted 18 August 2003 ; accepted in final form 28 November 2003 Rates of protein synthesis were investigated in skeletal muscles from rats submitted to chemical and surgical sympathectomy. Three models of sympathetic denervation were used: 1 ) treatment with guanethidine (100 mg·kg -1 ·day -1 sc); 2 ) lumbar sympathetic denervation (surgical excision of the second and third lumbar ganglia of the sympathetic chain, from which arises the postganglionic fibers to the skeletal muscles of rat hindlimb); and 3 ) adrenodemedullation. Protein synthesis was estimated in isolated soleus muscle by the rate of incorporation of [ 14 C]tyrosine (0.1 mM, 0.05 µCi/ml) into total protein. Soleus isolated after 2 and 4 days of chemical sympathectomy or after 3 days of lumbar denervation showed a 17-20% statistically significant decrease in in vitro rates of protein synthesis. These effects were reverted by addition of 10 -5 M isoproterenol or epinephrine in vitro. Neither clenbuterol nor isoproterenol (10 -7 , 10 -6 , or 10 -5 M) in vitro affected the rate of protein synthesis in soleus from normal rats. On the other hand, clenbuterol or epinephrine (10 -5 M) increased by 20% the rate of protein synthesis in soleus muscles from adrenodemedullated rats and prevented its decrease in muscles from fasted rats. The data suggest that the sympathetic nervous system stimulates protein synthesis in oxidative muscles, probably through the activation of 2 -adrenoceptors, especially in situations of hormonal or nutritional deficiency. catecholamines; isoproterenol; clenbuterol; surgical and chemical sympathectomy Address for reprint requests and other correspondence: I. C. Kettelhut, Dept. of Biochemistry, School of Medicine, USP, 14049-900 Ribeirão Preto, SP, Brazil (E-mail: idckette{at}fmrp.usp.br ).
Rates of protein synthesis were investigated in skeletal muscles from rats submitted to chemical and surgical sympathectomy. Three models of sympathetic denervation were used: 1) treatment with guanethidine (100 mg.kg(-1).day(-1) sc); 2) lumbar sympathetic denervation (surgical excision of the second and third lumbar ganglia of the sympathetic chain, from which arises the postganglionic fibers to the skeletal muscles of rat hindlimb); and 3) adrenodemedullation. Protein synthesis was estimated in isolated soleus muscle by the rate of incorporation of [(14)C]tyrosine (0.1 mM, 0.05 microCi/ml) into total protein. Soleus isolated after 2 and 4 days of chemical sympathectomy or after 3 days of lumbar denervation showed a 17-20% statistically significant decrease in in vitro rates of protein synthesis. These effects were reverted by addition of 10(-5) M isoproterenol or epinephrine in vitro. Neither clenbuterol nor isoproterenol (10(-7), 10(-6), or 10(-5) M) in vitro affected the rate of protein synthesis in soleus from normal rats. On the other hand, clenbuterol or epinephrine (10(-5) M) increased by 20% the rate of protein synthesis in soleus muscles from adrenodemedullated rats and prevented its decrease in muscles from fasted rats. The data suggest that the sympathetic nervous system stimulates protein synthesis in oxidative muscles, probably through the activation of beta(2)-adrenoceptors, especially in situations of hormonal or nutritional deficiency.
Author Navegantes, Luiz Carlos C
Resano, Neusa M. Z
Baviera, Amanda M
Kettelhut, Isis C
Migliorini, Renato H
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Snippet Department of Physiology, Biochemistry and Immunology, School of Medicine, University of São Paulo, 14049-900 Ribeirão Preto, São Paulo, Brazil Submitted 18...
Rates of protein synthesis were investigated in skeletal muscles from rats submitted to chemical and surgical sympathectomy. Three models of sympathetic...
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SubjectTerms Adrenal Medulla - drug effects
Adrenal Medulla - metabolism
Adrenergic Agents - pharmacology
Adrenergic beta-Agonists - pharmacology
Animals
Blood Glucose - metabolism
Catecholamines - blood
Catecholamines - metabolism
Catecholamines - pharmacology
Clenbuterol - pharmacology
Corticosterone - blood
Fasting - metabolism
Guanethidine - pharmacology
Isoproterenol - pharmacology
Kinetics
Male
Muscle Proteins - biosynthesis
Muscle, Skeletal - drug effects
Muscle, Skeletal - innervation
Muscle, Skeletal - metabolism
Rats
Rats, Wistar
Sympathectomy
Tyrosine - metabolism
Title Effect of sympathetic denervation on the rate of protein synthesis in rat skeletal muscle
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