A Simple BODIPY-Based Viscosity Probe for Imaging of Cellular Viscosity in Live Cells

Intracellular viscosity is a fundamental physical parameter that indicates the functioning of cells. In this work, we developed a simple boron-dipyrromethene (BODIPY)-based probe, BTV, for cellular mitochondria viscosity imaging by coupling a simple BODIPY rotor with a mitochondria-targeting unit. T...

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Published inSensors (Basel, Switzerland) Vol. 16; no. 9; p. 1397
Main Authors Su, Dongdong, Teoh, Chai, Gao, Nengyue, Xu, Qing-Hua, Chang, Young-Tae
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 31.08.2016
MDPI
Subjects
BODIPY
Boron Compounds - chemistry
cell imaging
Cell Survival
Coupling
FLIM
Fluorescence
fluorescent probes
HeLa Cells
Humans
Imaging
Microscopy
Microscopy, Fluorescence
Molecular Probes - chemistry
Monitoring
Optical Imaging - methods
Physical properties
sensors
Solvents
Solvents - chemistry
Spectrometry, Fluorescence
Viscosity
cell imaging
BODIPY
viscosity
FLIM
sensors
fluorescent probes
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Summary:Intracellular viscosity is a fundamental physical parameter that indicates the functioning of cells. In this work, we developed a simple boron-dipyrromethene (BODIPY)-based probe, BTV, for cellular mitochondria viscosity imaging by coupling a simple BODIPY rotor with a mitochondria-targeting unit. The BTV exhibited a significant fluorescence intensity enhancement of more than 100-fold as the solvent viscosity increased. Also, the probe showed a direct linear relationship between the fluorescence lifetime and the media viscosity, which makes it possible to trace the change of the medium viscosity. Furthermore, it was demonstrated that BTV could achieve practical applicability in the monitoring of mitochondrial viscosity changes in live cells through fluorescence lifetime imaging microscopy (FLIM).
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These authors contributed equally to this work.
ISSN:1424-8220
1424-8220
DOI:10.3390/s16091397