Detection of the Vascular Endothelial Growth Factor with a Novel Bioluminescence Resonance Energy Transfer Pair Using a Two-Component System

• Published in: Sensors (Basel, Switzerland) Vol. 17; no. 1; p. 145
• Main Authors: Wimmer, Tobias, Schroeter, Eva, Lorenz, Birgit, Stieger, Knut
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 13.01.2017; MDPI
• Subjects: Binding energy; Bioluminescence; Biosensors; BRET; Energy; Energy transfer; Enzymes; Fluorescence; Growth factors; Implantation; In vivo methods and tests; Kinases; neuropilin; Patients; PerCP-Cy5.5; Proteins; ranibizumab; RLuc8; Sensors; single chain variable fragment (scFv); Vascular endothelial growth factor; VEGF; BRET; single chain variable fragment (scFv); VEGF; RLuc8; PerCP-Cy5.5; neuropilin; ranibizumab

In this paper we describe a two-component BRET (bioluminescence resonance energy transfer)-based method to detect vascular endothelial growth factor (VEGF) molecules in unknown samples as the basis for subsequent in vivo use. A luminescent VEGF binding molecule, which binds in the receptor binding m...