Methods to Measure Antibody Neutralization of Live Human Coronavirus OC43

• Published in: Viruses Vol. 13; no. 10; p. 2075
• Main Authors: Boonyaratanakornkit, Jim, Sholukh, Anton M., Gray, Matthew, Bossard, Emily L., Ford, Emily S., Corbett, Kizzmekia S., Corey, Lawrence, Taylor, Justin J.
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 14.10.2021; MDPI
• Subjects: Antibodies; Antibodies, Monoclonal - immunology; Antibodies, Neutralizing - immunology; Antibodies, Viral - immunology; Betacoronavirus 1; Bronchopulmonary infection; Cell Line; Common Cold - immunology; Common Cold - pathology; Common Cold - virology; Coronavirus Infections - immunology; Coronavirus Infections - pathology; Coronavirus Infections - virology; Coronavirus OC43, Human - immunology; Coronaviruses; COVID-19; cytopathogenicity; Enzyme-linked immunosorbent assay; Enzyme-Linked Immunosorbent Assay - methods; Hematopoietic stem cells; human coronavirus; Human subjects; Humans; lungs; Medical research; Monoclonal antibodies; mortality; neutralization; neutralization assays; neutralization tests; Neutralization Tests - methods; neutralizing antibodies; OC43; Pandemics; Proteins; Severe acute respiratory syndrome coronavirus 2; Spike Glycoprotein, Coronavirus - immunology; Spike protein; Stem cell transplantation; Viral infections; Viruses; United States > US; neutralization assays; neutralizing antibodies; human coronavirus; OC43

The human Betacoronavirus OC43 is a common cause of respiratory viral infections in adults and children. Lung infections with OC43 are associated with mortality, especially in hematopoietic stem cell transplant recipients. Neutralizing antibodies play a major role in protection against many respirat...