Evaluation and Updating of the Osiris Expert System for Identification of Escherichia coli β-Lactam Resistance Phenotypes

Article Usage Stats Services JCM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue JCM About JCM Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Com...

Full description

Saved in:
Bibliographic Details
Published inJournal of Clinical Microbiology Vol. 43; no. 4; pp. 1846 - 1850
Main Authors BERT, Frédéric, JUVIN, Manette, OULD-HOCINE, Zahia, CLAREBOUT, Gervais, KELLER, Emmanuelle, LAMBERT, Nicole, ARLET, Guillaume
Format Journal Article
LanguageEnglish
Published Washington, DC American Society for Microbiology 01.04.2005
Subjects
Anti-Bacterial Agents - pharmacology
Bacteriology
beta-Lactam Resistance
beta-Lactamases - genetics
beta-Lactamases - metabolism
beta-Lactams - pharmacology
Biological and medical sciences
Escherichia coli
Escherichia coli - classification
Escherichia coli - drug effects
Fundamental and applied biological sciences. Psychology
Genotype
Humans
Infectious diseases
Medical sciences
Microbial Sensitivity Tests - instrumentation
Microbial Sensitivity Tests - methods
Microbiology
Miscellaneous
Phenotype
Video Recording - instrumentation
Video Recording - methods
Resistance
Microbiology
Escherichia coli
β-Lactams
Bacteria
Identification
Enterobacteriaceae
Online AccessGet full text

Cover

Abstract Article Usage Stats Services JCM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue JCM About JCM Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy JCM RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0095-1137 Online ISSN: 1098-660X Copyright © 2014 by the American Society for Microbiology.   For an alternate route to JCM .asm.org, visit: JCM       
AbstractList ABSTRACT Osiris is a video zone size reader for disk diffusion tests that includes a built-in extended expert system (EES). We evaluated the efficacy of the Osiris EES for the identification of β-lactam susceptibility phenotypes of Escherichia coli isolates. Fifteen β-lactam agents and three β-lactam-β-lactamase inhibitor combinations were tested by the disk diffusion method against 50 E. coli strains with well-characterized resistance mechanisms. The strains were screened for the production of extended-spectrum β-lactamase (ESBL) by the double-disk synergy test using a disk of amoxicillin-clavulanic acid with disks of the extended-spectrum cephalosporins and aztreonam. Overall, the EES accurately identified the phenotype for 78% of the strains, indicated an inexact phenotype for 17% of the strains, and could not find a matching phenotype for the remaining 5% of the strains. The percentage of correct identification for each resistance mechanism was 100% for inhibitor-resistant TEM and for TEM plus cephalosporinase, 88.9% for TEM and for ESBL, 70.8% for cephalosporinase overproduction, and 25% for oxacillinase. The main cause of discrepancy was the misidentification of oxacillinase as inhibitor-resistant TEM. The conventional double-disk synergy test failed to detect ESBL production in two strains (one producing VEB-1 and one producing CTX-M-14), but synergy between cefepime and amoxicillin-clavulanic acid was visible after the distance between the disks was reduced to 20 mm. After the interpretative guidelines of the EES were updated according to our results, the percentage of correct phenotype identification increased from 78 to 96%.
Article Usage Stats Services JCM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue JCM About JCM Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy JCM RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0095-1137 Online ISSN: 1098-660X Copyright © 2014 by the American Society for Microbiology.   For an alternate route to JCM .asm.org, visit: JCM       
Osiris is a video zone size reader for disk diffusion tests that includes a built-in extended expert system (EES). We evaluated the efficacy of the Osiris EES for the identification of beta-lactam susceptibility phenotypes of Escherichia coli isolates. Fifteen beta-lactam agents and three beta-lactam-beta-lactamase inhibitor combinations were tested by the disk diffusion method against 50 E. coli strains with well-characterized resistance mechanisms. The strains were screened for the production of extended-spectrum beta-lactamase (ESBL) by the double-disk synergy test using a disk of amoxicillin-clavulanic acid with disks of the extended-spectrum cephalosporins and aztreonam. Overall, the EES accurately identified the phenotype for 78% of the strains, indicated an inexact phenotype for 17% of the strains, and could not find a matching phenotype for the remaining 5% of the strains. The percentage of correct identification for each resistance mechanism was 100% for inhibitor-resistant TEM and for TEM plus cephalosporinase, 88.9% for TEM and for ESBL, 70.8% for cephalosporinase overproduction, and 25% for oxacillinase. The main cause of discrepancy was the misidentification of oxacillinase as inhibitor-resistant TEM. The conventional double-disk synergy test failed to detect ESBL production in two strains (one producing VEB-1 and one producing CTX-M-14), but synergy between cefepime and amoxicillin-clavulanic acid was visible after the distance between the disks was reduced to 20 mm. After the interpretative guidelines of the EES were updated according to our results, the percentage of correct phenotype identification increased from 78 to 96%.
Osiris is a video zone size reader for disk diffusion tests that includes a built-in extended expert system (EES). We evaluated the efficacy of the Osiris EES for the identification of β-lactam susceptibility phenotypes of Escherichia coli isolates. Fifteen β-lactam agents and three β-lactam-β-lactamase inhibitor combinations were tested by the disk diffusion method against 50 E. coli strains with well-characterized resistance mechanisms. The strains were screened for the production of extended-spectrum β-lactamase (ESBL) by the double-disk synergy test using a disk of amoxicillin-clavulanic acid with disks of the extended-spectrum cephalosporins and aztreonam. Overall, the EES accurately identified the phenotype for 78% of the strains, indicated an inexact phenotype for 17% of the strains, and could not find a matching phenotype for the remaining 5% of the strains. The percentage of correct identification for each resistance mechanism was 100% for inhibitor-resistant TEM and for TEM plus cephalosporinase, 88.9% for TEM and for ESBL, 70.8% for cephalosporinase overproduction, and 25% for oxacillinase. The main cause of discrepancy was the misidentification of oxacillinase as inhibitor-resistant TEM. The conventional double-disk synergy test failed to detect ESBL production in two strains (one producing VEB-1 and one producing CTX-M-14), but synergy between cefepime and amoxicillin-clavulanic acid was visible after the distance between the disks was reduced to 20 mm. After the interpretative guidelines of the EES were updated according to our results, the percentage of correct phenotype identification increased from 78 to 96%.
Author Gervais Clarebout
Nicole Lambert
Manette Juvin
Emmanuelle Keller
Guillaume Arlet
Zahia Ould-Hocine
Frédéric Bert
AuthorAffiliation Service de Microbiologie, Hôpital Beaujon, Clichy, 1 Bio-Rad, Marnes-la-Coquette, 2 Service de Microbiologie, Hôpital Tenon, Paris, France 3
AuthorAffiliation_xml – name: Service de Microbiologie, Hôpital Beaujon, Clichy, 1 Bio-Rad, Marnes-la-Coquette, 2 Service de Microbiologie, Hôpital Tenon, Paris, France 3
Author_xml – sequence: 1
  givenname: Frédéric
  surname: BERT
  fullname: BERT, Frédéric
  organization: Service de Microbiologie, Hôpital Beaujon, Clichy, France
– sequence: 2
  givenname: Manette
  surname: JUVIN
  fullname: JUVIN, Manette
  organization: Bio-Rad, Mames-la-Coquette, France
– sequence: 3
  givenname: Zahia
  surname: OULD-HOCINE
  fullname: OULD-HOCINE, Zahia
  organization: Service de Microbiologie, Hôpital Tenon, Paris, France
– sequence: 4
  givenname: Gervais
  surname: CLAREBOUT
  fullname: CLAREBOUT, Gervais
  organization: Bio-Rad, Mames-la-Coquette, France
– sequence: 5
  givenname: Emmanuelle
  surname: KELLER
  fullname: KELLER, Emmanuelle
  organization: Bio-Rad, Mames-la-Coquette, France
– sequence: 6
  givenname: Nicole
  surname: LAMBERT
  fullname: LAMBERT, Nicole
  organization: Service de Microbiologie, Hôpital Beaujon, Clichy, France
– sequence: 7
  givenname: Guillaume
  surname: ARLET
  fullname: ARLET, Guillaume
  organization: Service de Microbiologie, Hôpital Tenon, Paris, France
BackLink http://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16687100$$DView record in Pascal Francis
https://www.ncbi.nlm.nih.gov/pubmed/15815007$$D View this record in MEDLINE/PubMed
BookMark eNqFkcuO0zAYRi00iOkMvAKYBewS7PiaDRKqCgwqGgSMxM5yfGk8SuJgpwPlsXgQnolUrSisWFmWz_f5t88FOBvi4AB4glGJcSVfvFu-LykpaYkl5QWWDJUVQuweWGBUy4Jz9OUMLBCqWYExEefgIudbhDCljD0A55hJzBASC_Bjdae7rZ5CHKAeLLwZ7bwZNjB6OLUOXueQQoar76NLE_y0y5ProY8JXlk3TMEHc8jO-Cqb1qVg2qChiV2Av34Wa20m3cOPLoc86cE4-KF1Q5x2o8sPwX2vu-weHddLcPN69Xn5tlhfv7lavloXhqFqKrhupLUVF1Rb3zhe2caYRjdWIiKswaxuvOWEYt9wVlMsHDK1F5LZRkhfOXIJXh56x23TO2vmuZPu1JhCr9NORR3UvydDaNUm3imMJCYczwXPjwUpft26PKk-ZOO6Tg8ubrPiQmBCmPgviAWtSS34DIoDaFLMOTn_ZxqM1F6wmgUrShRVe8FqL1jtBc_Jx38_5pQ7Gp2BZ0dAZ6M7n-ZfD_nEcS4FRmjmnh64NmzabyE5pXOvbk1_upb8BveYwFA
CODEN JCMIDW
CitedBy_id crossref_primary_10_1128_JCM_00516_10
crossref_primary_10_1111_j_1399_3062_2011_00618_x
crossref_primary_10_1016_S1995_7645_10_60159_9
crossref_primary_10_1128_CMR_00061_10
crossref_primary_10_1007_s12088_012_0326_3
Cites_doi 10.1128/JCM.41.8.3712-3718.2003
10.1128/CMR.8.4.557
10.1128/AAC.46.12.3739-3743.2002
10.2307/30141529
10.1159/000077282
10.1128/AAC.44.10.2709-2714.2000
10.1093/clinids/10.4.867
10.1128/CMR.14.4.933-951.2001
10.1111/j.1574-6968.2002.tb11126.x
10.1128/JCM.40.11.4030-4036.2002
10.1128/AAC.45.6.1615-1620.2001
10.1128/JCM.39.1.175-182.2001
10.1093/jac/34.5.707
10.1128/AAC.47.7.2382-2383.2003
10.1128/JCM.38.2.542-546.2000
10.1128/AAC.39.11.2478
10.1128/AAC.46.1.1-11.2002
10.1128/AAC.46.3.630-637.2002
ContentType Journal Article
Copyright 2005 INIST-CNRS
Copyright © 2005, American Society for Microbiology 2005
Copyright_xml – notice: 2005 INIST-CNRS
– notice: Copyright © 2005, American Society for Microbiology 2005
DBID IQODW
CGR
CUY
CVF
ECM
EIF
NPM
AAYXX
CITATION
7QL
C1K
7X8
5PM
DOI 10.1128/JCM.43.4.1846-1850.2005
DatabaseName Pascal-Francis
Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
CrossRef
Bacteriology Abstracts (Microbiology B)
Environmental Sciences and Pollution Management
MEDLINE - Academic
PubMed Central (Full Participant titles)
DatabaseTitle MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
CrossRef
Bacteriology Abstracts (Microbiology B)
Environmental Sciences and Pollution Management
MEDLINE - Academic
DatabaseTitleList CrossRef

MEDLINE
Bacteriology Abstracts (Microbiology B)
MEDLINE - Academic
Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 2
  dbid: EIF
  name: MEDLINE
  url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search
  sourceTypes: Index Database
DeliveryMethod fulltext_linktorsrc
Discipline Medicine
Biology
EISSN 1098-660X
EndPage 1850
ExternalDocumentID 10_1128_JCM_43_4_1846_1850_2005
15815007
16687100
jcm_43_4_1846
Genre Evaluation Studies
Journal Article
GroupedDBID ---
.55
.GJ
08R
0R~
18M
29K
2WC
39C
3O-
4.4
41~
53G
5GY
5RE
5VS
AAUGY
AAYOK
ABOCM
ABPPZ
ACGFO
ADBBV
AENEX
AFMIJ
AGCDD
AI.
ALMA_UNASSIGNED_HOLDINGS
AOIJS
BAWUL
BTFSW
CS3
D-I
DIK
DU5
E3Z
EBS
EJD
F5P
FRP
GX1
H13
HF~
HYE
HZ~
H~9
IQODW
KQ8
L7B
O9-
OHT
OK1
P2P
P6G
RHF
RHI
RNS
RPM
RSF
TR2
UCJ
VH1
W8F
WHG
WOQ
X7M
ZA5
ZCA
ZGI
ZXP
~KM
AGVNZ
CGR
CUY
CVF
ECM
EIF
NPM
AAYXX
CITATION
7QL
C1K
7X8
5PM
ID FETCH-LOGICAL-c502t-6ab8dd2674adfbe62dbccbabd8037dc159bfd6341fb659417e0c9f785db78f2e3
IEDL.DBID RPM
ISSN 0095-1137
IngestDate Tue Sep 17 21:22:11 EDT 2024
Fri Aug 16 01:13:47 EDT 2024
Fri Aug 16 09:55:36 EDT 2024
Thu Sep 12 17:46:07 EDT 2024
Sat Sep 28 07:45:24 EDT 2024
Sun Oct 22 16:05:09 EDT 2023
Wed May 18 15:27:28 EDT 2016
IsDoiOpenAccess false
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 4
Keywords Resistance
Microbiology
Escherichia coli
β-Lactams
Bacteria
Identification
Enterobacteriaceae
Language English
License CC BY 4.0
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c502t-6ab8dd2674adfbe62dbccbabd8037dc159bfd6341fb659417e0c9f785db78f2e3
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Corresponding author. Mailing address: Service de Microbiologie, Hôpital Beaujon, 100 boulevard du Général Leclerc, 92110 Clichy, France. Phone: 33 1 40 87 54 42. Fax: 33 1 40 87 05 50. E-mail: frederic.bert@bjn.ap-hop-paris.fr.
OpenAccessLink https://europepmc.org/articles/pmc1081361?pdf=render
PMID 15815007
PQID 17493976
PQPubID 23462
PageCount 5
ParticipantIDs crossref_primary_10_1128_JCM_43_4_1846_1850_2005
pascalfrancis_primary_16687100
pubmed_primary_15815007
proquest_miscellaneous_67713357
proquest_miscellaneous_17493976
pubmedcentral_primary_oai_pubmedcentral_nih_gov_1081361
highwire_asm_jcm_43_4_1846
PublicationCentury 2000
PublicationDate 2005-04-01
PublicationDateYYYYMMDD 2005-04-01
PublicationDate_xml – month: 04
  year: 2005
  text: 2005-04-01
  day: 01
PublicationDecade 2000
PublicationPlace Washington, DC
PublicationPlace_xml – name: Washington, DC
– name: United States
PublicationTitle Journal of Clinical Microbiology
PublicationTitleAlternate J Clin Microbiol
PublicationYear 2005
Publisher American Society for Microbiology
Publisher_xml – name: American Society for Microbiology
References 3263690 - Rev Infect Dis. 1988 Jul-Aug;10(4):867-78
7706166 - J Antimicrob Chemother. 1994 Nov;34(5):707-14
12007800 - FEMS Microbiol Lett. 2002 Apr 9;209(2):161-8
15084803 - Chemotherapy. 2004 Apr;50(1):31-4
8585729 - Antimicrob Agents Chemother. 1995 Nov;39(11):2478-83
11136767 - J Clin Microbiol. 2001 Jan;39(1):175-82
12409370 - J Clin Microbiol. 2002 Nov;40(11):4030-6
11585791 - Clin Microbiol Rev. 2001 Oct;14(4):933-51, table of contents
11751104 - Antimicrob Agents Chemother. 2002 Jan;46(1):1-11
9778163 - Infect Control Hosp Epidemiol. 1998 Sep;19(9):653-6
11850241 - Antimicrob Agents Chemother. 2002 Mar;46(3):630-7
12821506 - Antimicrob Agents Chemother. 2003 Jul;47(7):2382-3
8665470 - Clin Microbiol Rev. 1995 Oct;8(4):557-84
10655342 - J Clin Microbiol. 2000 Feb;38(2):542-6
12904380 - J Clin Microbiol. 2003 Aug;41(8):3712-8
10991849 - Antimicrob Agents Chemother. 2000 Oct;44(10):2709-14
11353602 - Antimicrob Agents Chemother. 2001 Jun;45(6):1615-20
12435670 - Antimicrob Agents Chemother. 2002 Dec;46(12):3739-43
e_1_3_1_8_2
e_1_3_1_13_2
e_1_3_1_7_2
e_1_3_1_12_2
e_1_3_1_11_2
e_1_3_1_9_2
e_1_3_1_10_2
e_1_3_1_20_2
e_1_3_1_4_2
e_1_3_1_17_2
e_1_3_1_3_2
e_1_3_1_16_2
e_1_3_1_6_2
e_1_3_1_15_2
e_1_3_1_5_2
e_1_3_1_14_2
e_1_3_1_2_2
e_1_3_1_19_2
e_1_3_1_18_2
References_xml – ident: e_1_3_1_3_2
  doi: 10.1128/JCM.41.8.3712-3718.2003
– ident: e_1_3_1_14_2
  doi: 10.1128/CMR.8.4.557
– ident: e_1_3_1_7_2
  doi: 10.1128/AAC.46.12.3739-3743.2002
– ident: e_1_3_1_16_2
  doi: 10.2307/30141529
– ident: e_1_3_1_4_2
  doi: 10.1159/000077282
– ident: e_1_3_1_9_2
– ident: e_1_3_1_13_2
  doi: 10.1128/AAC.44.10.2709-2714.2000
– ident: e_1_3_1_12_2
  doi: 10.1093/clinids/10.4.867
– ident: e_1_3_1_6_2
  doi: 10.1128/CMR.14.4.933-951.2001
– ident: e_1_3_1_18_2
  doi: 10.1111/j.1574-6968.2002.tb11126.x
– ident: e_1_3_1_5_2
  doi: 10.1128/JCM.40.11.4030-4036.2002
– ident: e_1_3_1_2_2
  doi: 10.1128/AAC.45.6.1615-1620.2001
– ident: e_1_3_1_10_2
  doi: 10.1128/JCM.39.1.175-182.2001
– ident: e_1_3_1_11_2
  doi: 10.1093/jac/34.5.707
– ident: e_1_3_1_15_2
  doi: 10.1128/AAC.47.7.2382-2383.2003
– ident: e_1_3_1_20_2
  doi: 10.1128/JCM.38.2.542-546.2000
– ident: e_1_3_1_19_2
  doi: 10.1128/AAC.39.11.2478
– ident: e_1_3_1_17_2
  doi: 10.1128/AAC.46.1.1-11.2002
– ident: e_1_3_1_8_2
  doi: 10.1128/AAC.46.3.630-637.2002
SSID ssj0014455
Score 1.8538634
Snippet Article Usage Stats Services JCM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley...
Osiris is a video zone size reader for disk diffusion tests that includes a built-in extended expert system (EES). We evaluated the efficacy of the Osiris EES...
ABSTRACT Osiris is a video zone size reader for disk diffusion tests that includes a built-in extended expert system (EES). We evaluated the efficacy of the...
SourceID pubmedcentral
proquest
crossref
pubmed
pascalfrancis
highwire
SourceType Open Access Repository
Aggregation Database
Index Database
Publisher
StartPage 1846
SubjectTerms Anti-Bacterial Agents - pharmacology
Bacteriology
beta-Lactam Resistance
beta-Lactamases - genetics
beta-Lactamases - metabolism
beta-Lactams - pharmacology
Biological and medical sciences
Escherichia coli
Escherichia coli - classification
Escherichia coli - drug effects
Fundamental and applied biological sciences. Psychology
Genotype
Humans
Infectious diseases
Medical sciences
Microbial Sensitivity Tests - instrumentation
Microbial Sensitivity Tests - methods
Microbiology
Miscellaneous
Phenotype
Video Recording - instrumentation
Video Recording - methods
Title Evaluation and Updating of the Osiris Expert System for Identification of Escherichia coli β-Lactam Resistance Phenotypes
URI http://jcm.asm.org/content/43/4/1846.abstract
https://www.ncbi.nlm.nih.gov/pubmed/15815007
https://search.proquest.com/docview/17493976
https://search.proquest.com/docview/67713357
https://pubmed.ncbi.nlm.nih.gov/PMC1081361
Volume 43
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3LjtMwFLWmI4HYIBheGaB4wTZtUj-zRFWH0YjCCFGpO8uPmAmiaUUyC_gsPoRv4tpJplMEG9ZxIiXnOvdc-_hchF47R6TXGUu5pS6lJclTzbxMvZQWYshARRBVvu_5-YperNn6CLHhLEwU7VtTTeqvm0ldXUVt5W5jp4NObHq5nOeQxwjPpyM0EoQMJXq_dUAp69oWFCzNcyJ6URf8h6cX8-WEkgmdQFnDU8hUWVxUia15JDCj0FP2dnYaHIODYFI38M181-zib2z0T1HlrSx19gDd7-klftO9xkN0VNYn6E7XcPL7Cbq77LfSH6Efixufb6xrh1e7cNCh_oy3HgMpxB-aCuY_jlbILe6MzTEwXNwd7fX9Wl8YvmgC8lVQTWOIqwr_-pm-07bVG_yxbAJBhcjCl1dlvQ0rvs1jtDpbfJqfp30jhtSybNamXBvp3IwLqp03JZ85Y63RxsmMCGeBERnvOORDbzgraC7KzBZeSOaMkH5WkifouN7W5TOETWYK5rUjQmggB8GdDxiVccR6nTthEpQNAKhd57ehYp0ykwrgU5QoqgJ8KsAXmmiyBJ0OQCndbNQXu9kPS9D4ALr9QzmXwdkoQa8GLBXMrLBdoutye90oqNWKwNb-PYKLUOIzkaCnHfb7p_fxlCBxEBU3A4Kr9-EVCPbo7t0H9-l_3_kc3Yv-slFe9AIdt9-uy5fAnFozRqO363wc58tvfr8Z8A
link.rule.ids 230,315,733,786,790,891,27955,27956,53825,53827
linkProvider National Library of Medicine
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3LctMwFNWUMjw2PMrLBVot2NrxS48smUw6oSSlwzRMdxo9LBrATgY7C_pZfAjfxJVsN00HFrC2rBnLR7rnSkfnIvTGmIxbGZOQ6tyEeZEloSSWh5ZzDRhSkBF4le8Jnczz43NyvoNIfxfGi_a1WkTVtzKqFhdeW7kq9aDXiQ1OZ6ME4lhGk8EtdBvma0r6JL07PMhz0hYuGJIwSTLWybpgJR4cj2ZRnkV5BIkNDSFWxX5bxRfn4cCNXFXZ6_Gp9wx2kklZw6jZttzFn_joTVnltTh19BB96r-wlad8jdaNivTlDfPHfx6CR-hBx1zx2_bxY7RTVHvoTlvL8sceujvrTumfoMvxlYU4lpXB85W7Q1F9xkuLgW_iD_UClhbsXZYb3HqmYyDPuL01bLttRNd8XDtQLZwgGwNkF_jXz3AqdSNL_LGoHfcF0OLTi6Jaus3k-imaH43PRpOwq_EQahKnTUil4saklOXSWFXQ1CitlVSGxxkzGsiWsoZCqLWKkmGesCLWQ8s4MYpxmxbZM7RbLaviBcIqVkNipckYk8A7nPEfkDVlMm1lYpgKUNz_WbFqrTyET4FSLgAXIs9ELhwuhMOFq89JArTfI0DIuhRfdLlpFqCDLUxsOqWUO9OkAB32IBEwad1JjKyK5boWkAYOHRH8ewvK3O4BYQF63oJq03sH1ACxLbhdNXCG4dtPAETeOLwDzf5_v3mI7k3OZlMxfXfy_iW6721svYrpFdptvq-L10DQGnXgp-NvSsw66Q
linkToPdf http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3LbtNAFB1BEVU3PMrLBdpZsLXj1zyyRCFRKU2JEJEqNqN5eNpA40TYWdDP4kP4Ju6M7SapYNO1ry15fGbuuTPH5yL0zpiMWxmTkOrchHmRJaEkloeWcw0YUlAReJXvGT2e5ifn5Hyj1ZcX7Ws1i8qreVTOLr22cjnXvU4n1puMBwnksYwmvaWxvfvoAczZlHWFenuAkOekaV7QJ2GSZKyVdsFq3DsZjKM8i_IIihsaQr6K_daKb9DDgR-5zrKbOarzDXaySVnByNmm5cW_OOltaeVGrho9Rt-6t2wkKj-iVa0ifX3LAPJOw_AEPWoZLH7fhDxF94pyHz1selr-2ke74_a0_hm6Ht5YiWNZGjxdun8pygu8sBh4J_5czWCJwd5tucaNdzoGEo2bv4dtu53owoeVA9fMCbMxQHeG__wOT6Wu5Rx_KSrHgQG8eHJZlAu3qVw9R9PR8OvgOGx7PYSaxGkdUqm4MSlluTRWFTQ1SmslleFxxowG0qWsoZByraKknyesiHXfMk6MYtymRfYC7ZSLsniFsIpVn1hpMsYk8A9nAAikTZlMW5kYpgIUd19XLBtLD-FLoZQLwIbIM5ELhw3hsOH6dJIAHXQoELKai-96vg4L0OEWLtYPpZQ786QAHXVAETB53YmMLIvFqhJQDvYdIfx_BGVuF4GwAL1sgLV-egvWALEtyN0EOOPw7SsAJG8g3gLn4M53HqHdyYeROP149uk12vNutl7M9Abt1D9XxVvgabU69DPyLwkVPWk
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Evaluation+and+updating+of+the+osiris+expert+system+for+identification+of+Escherichia+coli+%CE%B2-lactam+resistance+phenotypes&rft.jtitle=Journal+of+clinical+microbiology&rft.au=BERT%2C+Fr%C3%A9d%C3%A9ric&rft.au=JUVIN%2C+Manette&rft.au=OULD-HOCINE%2C+Zahia&rft.au=CLAREBOUT%2C+Gervais&rft.date=2005-04-01&rft.pub=American+Society+for+Microbiology&rft.issn=0095-1137&rft.eissn=1098-660X&rft.volume=43&rft.issue=4&rft.spage=1846&rft.epage=1850&rft_id=info:doi/10.1128%2FJCM.43.4.1846-1850.2005&rft.externalDBID=n%2Fa&rft.externalDocID=16687100
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0095-1137&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0095-1137&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0095-1137&client=summon