The Pathogen–Host Interface in Three Dimensions: Correlative FIB/SEM Applications

• Published in: Trends in microbiology (Regular ed.) Vol. 27; no. 5; pp. 426 - 439
• Main Authors: Weiner, Allon, Enninga, Jost
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.05.2019; Elsevier Science Ltd; Elsevier
• Subjects: Bacteriology; Brucella; Cell Behavior; Cellular Biology; Correlation; correlative microscopy; FIB/SEM; Fluorescence; Fluorescence microscopy; Genetics; host-pathogen relationships; Human genetics; Human health and pathology; Infectious diseases; Intracellular; Ion beams; Life Sciences; Microbiology and Parasitology; Pathogens; Pulmonology and respiratory tract; Salmonella; Scanning electron microscopy; Shigella; tomography; Ultrastructure; volume electron microscopy; Salmonella; correlative microscopy; volume electron microscopy; FIB/SEM; Shigella; Brucella

Pathogens survive and propagate within host cells through a wide array of complex interactions. Tracking the molecular and cellular events by multidimensional fluorescence microscopy has been a widespread tool for research on intracellular pathogens. Through major advancements in 3D electron microsc...