In Vitro and In Vivo Phenotypes of Venezuelan, Eastern and Western Equine Encephalitis Viruses Derived from cDNA Clones of Human Isolates

• Published in: Viruses Vol. 15; no. 1; p. 5
• Main Authors: Gardner, Christina L., Sun, Chengqun, Dunn, Matthew D., Gilliland, Theron C., Trobaugh, Derek W., Terada, Yutaka, Reed, Douglas S., Hartman, Amy L., Klimstra, William B.
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 20.12.2022; MDPI
• Subjects: Aerosols; Alphavirus; Animal models; Animals; cDNA clone; Cell culture; Clone Cells; Cloning; DNA, Complementary - genetics; Encephalitis; Encephalitis Virus, Venezuelan Equine; Encephalitis Virus, Western Equine; equine; Expression vectors; FDA approval; fluorescence; gene expression; Genomes; Heparan sulfate; Horses; human isolate; Humans; Infections; Inoculation; Laboratory animals; Mice; Mortality; Mutagenesis; Mutation; Phenotype; Phenotypes; Propagation; Replication; Serology; Strains (organisms); therapeutics; Tissue culture; United States; Vaccines; viral genome; Virulence; virus replication; Viruses; Western equine encephalitis; wild type; United States; United States > US; FDA; cDNA clone; encephalitis; wild type; alphavirus; human isolate; equine

The Department of Defense recently began an effort to improve and standardize virus challenge materials and efficacy determination strategies for testing therapeutics and vaccines. This includes stabilization of virus genome sequences in cDNA form where appropriate, use of human-derived virus isolat...