Assessment of Metagenomic Sequencing and qPCR for Detection of Influenza D Virus in Bovine Respiratory Tract Samples - Zhang, Maodong; Huang, Yanyun; Godson, Dale L.; Fernando, Champika; Alexander, Trevor W.; Hill, Janet E. (Viruses, 2020)
Keywords: Animals; Antibodies, Viral - blood; Bioinformatics; bovine respiratory disease; Cattle; Cattle Diseases - diagnosis; Cattle Diseases - virology; Computational Biology; Dairy cattle; Deoxyribonucleic acid; diagnostics; DNA; Enzymes; Genome, Viral; Genomes; high-throughput nucleotide sequencing; High-Throughput Nucleotide Sequencing - methods; Illumina MiSeq sequencing; Influenza; Influenza D virus; Laboratory animals; Library collections; Metagenome; Metagenomics; nanopore GridION sequencing; Nanopores; Next-generation sequencing; nose; Orthomyxoviridae Infections - diagnosis; Orthomyxoviridae Infections - veterinary; Orthomyxoviridae Infections - virology; qPCR; quantitative polymerase chain reaction; Real-Time Polymerase Chain Reaction - methods; Respiratory tract; Respiratory Tract Infections - diagnosis; Respiratory Tract Infections - veterinary; Respiratory Tract Infections - virology; RNA, Viral - genetics; Sensitivity and Specificity; Thogotovirus - genetics; Thogotovirus - isolation & purification; Viruses; United States--US; bovine respiratory disease; influenza D virus; nanopore GridION sequencing; diagnostics; qPCR; Illumina MiSeq sequencing