The effect of hypoxia on human trophoblast in culture: Morphology, glucose transport and metabolism

• Published in: Placenta (Eastbourne) Vol. 18; no. 2; pp. 129 - 136
• Main Authors: Esterman, A., Greco, M.A., Mitani, Y., Finlay, T.H., Ismail-Beigi, F., Dancis, J.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.03.1997; Elsevier
• Subjects: Biological and medical sciences; Biological Transport; Blotting, Northern; Cell Division; Cell Hypoxia; Cells, Cultured; Embryology: invertebrates and vertebrates. Teratology; Fetal membranes; Fundamental and applied biological sciences. Psychology; General aspects. Development. Fetal membranes; Glucose - metabolism; Humans; Microscopy, Electron; Trophoblasts - cytology; Trophoblasts - metabolism; Trophoblasts - ultrastructure; Human; Trophoblaste; Cell culture; Oxygen; Fetal membrane; Biological transport; Environmental factor; Glucose; Metabolism; In vitro; Pregnancy; Placenta; Morphology; Hypoxia

The response to hypoxia of trophoblast isolated from term placenta and maintained in culture was studied. Trophoblast exposed to normoxic ( PO 2 120–130 mmHg) or hypoxic ( PO 2 12–14 mmHg) conditions were examined by electron microscopy. After 48 h, the cytoplasm of the hypoxic cells was more electr...