Isolation and Characterisation of Alongshan Virus in Russia
In recent decades, many new flavi-like viruses have been discovered predominantly in different invertebrates and, as was recently shown, some of them may cause disease in humans. The Jingmenvirus (JMV) group holds a special place among flaviviruses and flavi-like viruses because they have a segmente...
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| Published in | Viruses Vol. 12; no. 4; p. 362 |
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| Main Authors | , , , , , , , , , , , , , , , , |
| Format | Journal Article |
| Language | English |
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26.03.2020
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| Abstract | In recent decades, many new flavi-like viruses have been discovered predominantly in different invertebrates and, as was recently shown, some of them may cause disease in humans. The Jingmenvirus (JMV) group holds a special place among flaviviruses and flavi-like viruses because they have a segmented ssRNA(+) genome. We detected Alongshan virus (ALSV), which is a representative of the JMV group, in ten pools of adult Ixodes persulcatus ticks collected in two geographically-separated Russian regions. Three of the ten strains were isolated in the tick cell line IRE/CTVM19. One of the strains persisted in the IRE/CTVM19 cells without cytopathic effect for three years. Most ALSV virions purified from tick cells were spherical with a diameter of approximately 40.5 nm. In addition, we found smaller particles of approximately 13.1 nm in diameter. We obtained full genome sequences of all four segments of two of the isolated ALSV strains, and partial sequences of one segment from the third strain. Phylogenetic analysis on genome segment 2 of the JMV group clustered our novel strains with other ALSV strains. We found evidence for the existence of a novel upstream open reading frame in the glycoprotein-coding segment of ALSV and other members of the JMV group. |
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| AbstractList | In recent decades, many new flavi-like viruses have been discovered predominantly in different invertebrates and, as was recently shown, some of them may cause disease in humans. The Jingmenvirus (JMV) group holds a special place among flaviviruses and flavi-like viruses because they have a segmented ssRNA(+) genome. We detected Alongshan virus (ALSV), which is a representative of the JMV group, in ten pools of adult
Ixodes persulcatus
ticks collected in two geographically-separated Russian regions. Three of the ten strains were isolated in the tick cell line IRE/CTVM19. One of the strains persisted in the IRE/CTVM19 cells without cytopathic effect for three years. Most ALSV virions purified from tick cells were spherical with a diameter of approximately 40.5 nm. In addition, we found smaller particles of approximately 13.1 nm in diameter. We obtained full genome sequences of all four segments of two of the isolated ALSV strains, and partial sequences of one segment from the third strain. Phylogenetic analysis on genome segment 2 of the JMV group clustered our novel strains with other ALSV strains. We found evidence for the existence of a novel upstream open reading frame in the glycoprotein-coding segment of ALSV and other members of the JMV group. In recent decades, many new flavi-like viruses have been discovered predominantly in different invertebrates and, as was recently shown, some of them may cause disease in humans. The Jingmenvirus (JMV) group holds a special place among flaviviruses and flavi-like viruses because they have a segmented ssRNA(+) genome. We detected Alongshan virus (ALSV), which is a representative of the JMV group, in ten pools of adult Ixodes persulcatus ticks collected in two geographically-separated Russian regions. Three of the ten strains were isolated in the tick cell line IRE/CTVM19. One of the strains persisted in the IRE/CTVM19 cells without cytopathic effect for three years. Most ALSV virions purified from tick cells were spherical with a diameter of approximately 40.5 nm. In addition, we found smaller particles of approximately 13.1 nm in diameter. We obtained full genome sequences of all four segments of two of the isolated ALSV strains, and partial sequences of one segment from the third strain. Phylogenetic analysis on genome segment 2 of the JMV group clustered our novel strains with other ALSV strains. We found evidence for the existence of a novel upstream open reading frame in the glycoprotein-coding segment of ALSV and other members of the JMV group. In recent decades, many new flavi-like viruses have been discovered predominantly in different invertebrates and, as was recently shown, some of them may cause disease in humans. The Jingmenvirus (JMV) group holds a special place among flaviviruses and flavi-like viruses because they have a segmented ssRNA(+) genome. We detected Alongshan virus (ALSV), which is a representative of the JMV group, in ten pools of adult ticks collected in two geographically-separated Russian regions. Three of the ten strains were isolated in the tick cell line IRE/CTVM19. One of the strains persisted in the IRE/CTVM19 cells without cytopathic effect for three years. Most ALSV virions purified from tick cells were spherical with a diameter of approximately 40.5 nm. In addition, we found smaller particles of approximately 13.1 nm in diameter. We obtained full genome sequences of all four segments of two of the isolated ALSV strains, and partial sequences of one segment from the third strain. Phylogenetic analysis on genome segment 2 of the JMV group clustered our novel strains with other ALSV strains. We found evidence for the existence of a novel upstream open reading frame in the glycoprotein-coding segment of ALSV and other members of the JMV group. In recent decades, many new flavi-like viruses have been discovered predominantly in different invertebrates and, as was recently shown, some of them may cause disease in humans. The Jingmenvirus (JMV) group holds a special place among flaviviruses and flavi-like viruses because they have a segmented ssRNA(+) genome. We detected Alongshan virus (ALSV), which is a representative of the JMV group, in ten pools of adult Ixodes persulcatus ticks collected in two geographically-separated Russian regions. Three of the ten strains were isolated in the tick cell line IRE/CTVM19. One of the strains persisted in the IRE/CTVM19 cells without cytopathic effect for three years. Most ALSV virions purified from tick cells were spherical with a diameter of approximately 40.5 nm. In addition, we found smaller particles of approximately 13.1 nm in diameter. We obtained full genome sequences of all four segments of two of the isolated ALSV strains, and partial sequences of one segment from the third strain. Phylogenetic analysis on genome segment 2 of the JMV group clustered our novel strains with other ALSV strains. We found evidence for the existence of a novel upstream open reading frame in the glycoprotein-coding segment of ALSV and other members of the JMV group.In recent decades, many new flavi-like viruses have been discovered predominantly in different invertebrates and, as was recently shown, some of them may cause disease in humans. The Jingmenvirus (JMV) group holds a special place among flaviviruses and flavi-like viruses because they have a segmented ssRNA(+) genome. We detected Alongshan virus (ALSV), which is a representative of the JMV group, in ten pools of adult Ixodes persulcatus ticks collected in two geographically-separated Russian regions. Three of the ten strains were isolated in the tick cell line IRE/CTVM19. One of the strains persisted in the IRE/CTVM19 cells without cytopathic effect for three years. Most ALSV virions purified from tick cells were spherical with a diameter of approximately 40.5 nm. In addition, we found smaller particles of approximately 13.1 nm in diameter. We obtained full genome sequences of all four segments of two of the isolated ALSV strains, and partial sequences of one segment from the third strain. Phylogenetic analysis on genome segment 2 of the JMV group clustered our novel strains with other ALSV strains. We found evidence for the existence of a novel upstream open reading frame in the glycoprotein-coding segment of ALSV and other members of the JMV group. |
| Audience | Academic |
| Author | Gushchin, Vladimir A. Karganova, Galina G. Litov, Alexander G. Luchinina, Svetlana V. Yakovlev, Alexander S. Ivannikova, Anna Y. Belova, Oxana A. Bell-Sakyi, Lesley Gmyl, Anatoly P. Klimentov, Alexander S. Nikitin, Nikolai A. Bespyatova, Liubov A. Polienko, Alexandra E. Gmyl, Larissa V. Kholodilov, Ivan S. Bugmyrin, Sergey V. Shchetinin, Alexey M. |
| AuthorAffiliation | 6 Department of Infection Biology, Institute of Infection and Global Health, University of Liverpool, Liverpool L3 5RF, UK; l.bell-sakyi@liverpool.ac.uk 7 Laboratory for Animal and Plant Parasitology, Institute of Biology of Karelian Research Centre, Russian Academy of Sciences (IB KarRC RAS), 185910 Petrozavodsk, Russia; sbugmyr@mail.ru (S.V.B.); gamasina@mail.ru (L.A.B.) 8 Russian Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, 454092 Chelyabinsk, Russia; luchinina_sv@74.rospotrebnadzor.ru 2 Laboratory of Biochemistry, Chumakov Institute of Poliomyelitis and Viral Encephalitides, FSBSI Chumakov FSC R&D IBP RAS, 108819 Moscow, Russia; aklimentov@mail.ru (A.S.K.); lvgmyl@mail.ru (L.V.G.) 1 Laboratory of Biology of Arboviruses, Chumakov Institute of Poliomyelitis and Viral Encephalitides, FSBSI Chumakov FSC R&D IBP RAS, 108819 Moscow, Russia; ivan-kholodilov@bk.ru (I.S.K.); novosti-wxo@yandex.ru (A.G.L.); mikasusha@bk.ru (O.A.B.); polienko.ae@yandex.ru (A |
| AuthorAffiliation_xml | – name: 5 Pathogenic Microorganisms Variability Laboratory, Gamaleya Federal Research Centre for Epidemiology and Microbiology, Ministry of Health of the Russian Federation, 123098 Moscow, Russia; shchetinin.alexey@yandex.ru – name: 9 Department of Organization and Technology of Immunobiological Preparations, Institute for Translational Medicine and Biotechnology, Sechenov University, 119991 Moscow, Russia – name: 2 Laboratory of Biochemistry, Chumakov Institute of Poliomyelitis and Viral Encephalitides, FSBSI Chumakov FSC R&D IBP RAS, 108819 Moscow, Russia; aklimentov@mail.ru (A.S.K.); lvgmyl@mail.ru (L.V.G.) – name: 7 Laboratory for Animal and Plant Parasitology, Institute of Biology of Karelian Research Centre, Russian Academy of Sciences (IB KarRC RAS), 185910 Petrozavodsk, Russia; sbugmyr@mail.ru (S.V.B.); gamasina@mail.ru (L.A.B.) – name: 8 Russian Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, 454092 Chelyabinsk, Russia; luchinina_sv@74.rospotrebnadzor.ru – name: 6 Department of Infection Biology, Institute of Infection and Global Health, University of Liverpool, Liverpool L3 5RF, UK; l.bell-sakyi@liverpool.ac.uk – name: 1 Laboratory of Biology of Arboviruses, Chumakov Institute of Poliomyelitis and Viral Encephalitides, FSBSI Chumakov FSC R&D IBP RAS, 108819 Moscow, Russia; ivan-kholodilov@bk.ru (I.S.K.); novosti-wxo@yandex.ru (A.G.L.); mikasusha@bk.ru (O.A.B.); polienko.ae@yandex.ru (A.E.P.); strannotut@gmail.com (A.Y.I.); alex-montreal@yandex.ru (A.S.Y.); apgmyl@mail.ru (A.P.G.) – name: 3 Laboratory of Biology and Indication of Arboviruses, Department Ivanovsky Institute of Virology, Gamaleya Federal Research Centre for Epidemiology and Microbiology, Ministry of Health of the Russian Federation, 123098 Moscow, Russia – name: 4 Faculty of Biology, Lomonosov MSU, 119991 Moscow, Russia; nik.nikitin@gmail.com (N.A.N.); wowaniada@gmail.com (V.A.G.) |
| Author_xml | – sequence: 1 givenname: Ivan S. orcidid: 0000-0002-3764-7081 surname: Kholodilov fullname: Kholodilov, Ivan S. – sequence: 2 givenname: Alexander G. surname: Litov fullname: Litov, Alexander G. – sequence: 3 givenname: Alexander S. surname: Klimentov fullname: Klimentov, Alexander S. – sequence: 4 givenname: Oxana A. surname: Belova fullname: Belova, Oxana A. – sequence: 5 givenname: Alexandra E. surname: Polienko fullname: Polienko, Alexandra E. – sequence: 6 givenname: Nikolai A. orcidid: 0000-0001-9626-2336 surname: Nikitin fullname: Nikitin, Nikolai A. – sequence: 7 givenname: Alexey M. surname: Shchetinin fullname: Shchetinin, Alexey M. – sequence: 8 givenname: Anna Y. surname: Ivannikova fullname: Ivannikova, Anna Y. – sequence: 9 givenname: Lesley surname: Bell-Sakyi fullname: Bell-Sakyi, Lesley – sequence: 10 givenname: Alexander S. surname: Yakovlev fullname: Yakovlev, Alexander S. – sequence: 11 givenname: Sergey V. orcidid: 0000-0001-5285-6933 surname: Bugmyrin fullname: Bugmyrin, Sergey V. – sequence: 12 givenname: Liubov A. surname: Bespyatova fullname: Bespyatova, Liubov A. – sequence: 13 givenname: Larissa V. surname: Gmyl fullname: Gmyl, Larissa V. – sequence: 14 givenname: Svetlana V. surname: Luchinina fullname: Luchinina, Svetlana V. – sequence: 15 givenname: Anatoly P. surname: Gmyl fullname: Gmyl, Anatoly P. – sequence: 16 givenname: Vladimir A. orcidid: 0000-0002-9397-3762 surname: Gushchin fullname: Gushchin, Vladimir A. – sequence: 17 givenname: Galina G. surname: Karganova fullname: Karganova, Galina G. |
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| Keywords | Ixodes persulcatus Jingmen tick virus Alongshan virus flavi-like virus open reading frame tick cell line flavivirus |
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| Title | Isolation and Characterisation of Alongshan Virus in Russia |
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