Changes in Cytosolic pH within Arabidopsis Root Columella Cells Play a Key Role in the Early Signaling Pathway for Root Gravitropism

• Published in: Plant physiology (Bethesda) Vol. 121; no. 4; pp. 1291 - 1298
• Main Authors: Amie Caroline Scott, Allen, Nina Strömgren
• Format: Journal Article
• Language: English
• Published: Legacy CDMS American Society of Plant Physiologists 01.12.1999
• Subjects: Acidification; Arabidopsis - cytology; Arabidopsis - growth & development; Arabidopsis - physiology; Biological and medical sciences; Cell Biology and Signal Transduction; Curvature; Cytosol - physiology; Fundamental and applied biological sciences. Psychology; Gravitropism; Gravitropism - physiology; Gravity perception; Hydrogen-Ion Concentration; Kinetics; Life Sciences (General); Microscopy, Fluorescence; Movements; Plant cells; Plant physiology and development; Plant roots; Plant Roots - cytology; Plant Roots - growth & development; Plant Roots - physiology; Plants; Root cap; Root growth; Signal Transduction; Space life sciences; Time Factors; Nasa Discipline Plant Biology; Non-Nasa Center; Nasa Program Fundamental Space Biology; Tropism; Root; Early stage; Cytosol; Arabidopsis thaliana; Signal transduction; Cruciferae; Dicotyledones; Angiospermae; pH; Gravitropism; Spermatophyta; Experimental plant; NASA Program Fundamental Space Biology; NASA Discipline Plant Biology; Non-NASA Center

Ratiometric wide-field fluorescence microscopy with 1′,7′-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein (BCECF)-dextran demonstrated that gravistimulation leads to rapid changes in cytoplasmic pH (pHc) in columella cells of Arabidopsis roots. The pHc of unstimulated columella cells in tiers 2 an...