Grafting voltage and pharmacological sensitivity in potassium channels

• Published in: Cell research Vol. 26; no. 8; pp. 935 - 945
• Main Authors: Lan, Xi, Fan, Chunyan, Ji, Wei, Tian, Fuyun, Xu, Tao, Gao, Zhaobing
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.08.2016; Nature Publishing Group
• Subjects: 631/57/2272; 631/80/86; 631/92/269/1151; Animals; Biomedical and Life Sciences; Cell Biology; CHO Cells; Cricetinae; Cricetulus; Humans; Hydrogen-Ion Concentration; Ion Channel Gating - drug effects; Life Sciences; Membrane Transport Modulators - pharmacology; Original; original-article; Potassium Channels - chemistry; Potassium Channels - metabolism; Potassium Channels, Tandem Pore Domain - metabolism; Protein Domains; 嫁接; 敏感性; 电压传感; 电压依赖性; 电压门控; 离子通道; 药理; 钾通道; graft; K2P channels; voltage-gated channels; voltage-sensing domain

A classical voltage-gated ion channel consists of four voltage-sensing domains （VSDs）. However, the roles of each VSD in the channels remain elusive. We developed a GVTDT （Graft VSD To Dimeric TASK3 channels that lack endogenous VSDs） strategy to produce voltage-gated channels with a reduced number...