Establishment of a new reverse genetics system for respiratory syncytial virus under the control of RNA polymerase II

• Published in: Microbiology and immunology
• Main Authors: Takahashi, Tatsuki, Ueno, Shiori, Sugiura, Yoshiro, Shimizu, Kenta, Kamitani, Wataru
• Format: Journal Article
• Language: English
• Published: Australia 01.09.2023
• Subjects: Huh-7 cell; reverse genetics; RSV; 293T cell; respiratory syncytial virus; RNA polymerase II

A reverse genetics system for the respiratory syncytial virus (RSV), which causes acute respiratory illness, is an effective tool for understanding the pathogenicity of RSV. To date, a method dependent on T7 RNA polymerase is commonly used for RSV. Although this method is well established and recomb...