Determination of mycobiota and mycotoxins in pig feed in central Argentina

• Published in: Letters in applied microbiology Vol. 46; no. 5; pp. 555 - 561
• Main Authors: González Pereyra, M.L, Pereyra, C.M, Ramírez, M.L, Rosa, C.A.R, Dalcero, A.M, Cavaglieri, L.R
• Format: Journal Article
• Language: English
• Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01.05.2008; Blackwell Publishing Ltd; Blackwell Science
• Subjects: aflatoxins; Aflatoxins - analysis; Animal Feed - analysis; Animal Feed - microbiology; Animals; Argentina; Aspergillus - classification; Aspergillus - isolation & purification; Aspergillus flavus; Aspergillus parasiticus; Biological and medical sciences; Chromatography, High Pressure Liquid; Chromatography, Thin Layer; Edible Grain - microbiology; feedstuff; Food Contamination - analysis; fumonisins; Fumonisins - analysis; Fundamental and applied biological sciences. Psychology; Fusarium - classification; Fusarium - isolation & purification; Fusarium verticillioides; Microbiology; mycobiota; Mycological Typing Techniques; Mycotoxins - analysis; pigs; Stem Cells; Swine; Zea; zearalenone; Zearalenone - analysis; South America; Argentina; America; Applied microbiology; Mycotoxin; mycobiota; Zearalenone; feedstuff; Pig; Toxin; pigs; Vertebrata; aflatoxins; Mammalia; fumonisins; Artiodactyla; Fumonisin; Ungulata; Aflatoxin
• ISSN: 0266-8254; 1472-765X
• DOI: 10.1111/j.1472-765X.2008.02347.x

To evaluate the mycobiota and natural levels of aflatoxins, fumonisins and zearalenone present in compound feed and home-corn grains intended for fattening pigs. Total fungi, Fusarium and Aspergillus species occurrence were examined. Aflatoxins and zearalenone were detected by thin-layer chromatography and fumonisins by high-pressure liquid chromatography. Fungal counts were generally higher than 1 x 10⁵ colony forming units (CFU) ml⁻¹. Aspergillus flavus, Aspergillus parasiticus and Fusarium verticillioides were the most prevalent species. FB₁ and FB₂ were detected in all feed and corn samples. Aflatoxin B₁ was detected in 33·33% of initial and growing feed and in 44·44% of final feed samples. It was not detected in corn samples. All feed and corn samples were negative for AFB₂, AFG₁, AFG₂ and ZEA presence during all growing stages tested. Fungal counts at all growing periods exceeded the levels proposed as feed hygienic quality limits. Aflatoxin levels in all feeds and fumonisin levels in many samples were higher than the established regulations. The presence of mycotoxins indicates the existence of contamination. This fact requires periodic monitoring to prevent the occurrence of mycotoxicosis in animal production, to reduce the economic losses and to minimize hazards to human health.