Immunogold-silver staining of lymphocyte surface antigens on cells in suspension and in lymph node cryostat sections

• Published in: Journal of microscopy (Oxford) Vol. 143; no. Pt 2; p. 151
• Main Authors: De Waele, M, De Mey, J, Renmans, W, Labeur, C, Reynaert, P, Van Camp, B
• Format: Journal Article
• Language: English
• Published: England 01.08.1986
• Subjects: Antigens, Surface - analysis; Cell Membrane - immunology; Cell Membrane - ultrastructure; Fluorescent Antibody Technique; Gold; Humans; Immunoenzyme Techniques; Indicators and Reagents; Lymph Nodes - immunology; Lymphocytes - cytology; Lymphocytes - immunology; Silver; T-Lymphocytes - classification; T-Lymphocytes - immunology

An immunogold-silver staining (IGSS) technique for the light microscopical detection of leucocyte cell surface antigens in cell suspensions and cryostat sections is described. The specimens were first incubated with monoclonal mouse antibodies and then with colloidal gold-labelled goat anti-mouse an...