Production of high-titer transmission-defective RNA virus-based episomal vector using tangential flow filtration

• Published in: Microbiology and immunology Vol. 64; no. 9; p. 602
• Main Authors: Komatsu, Yumiko, Kakuya, Yoji, Tomonaga, Keizo
• Format: Journal Article
• Language: English
• Published: Australia 01.09.2020
• Subjects: Animals; Brain - virology; Cell Line; Chlorocebus aethiops; Female; Filtration - methods; Gene Expression; Gene Transfer Techniques; Genetic Vectors - administration & dosage; Genetic Vectors - isolation & purification; HEK293 Cells; Humans; Plasmids - genetics; Plasmids - isolation & purification; Pregnancy; Rats; Rats, Inbred Lew; RNA Viruses - genetics; RNA Viruses - isolation & purification; Transgenes; Vero Cells; Viral Load; in vivo gene delivery; viral vector; RNA virus-based episomal vector; tangential flow filtration; high-titer

In recent years, viral vector based in vivo gene delivery strategies have achieved a significant success in the treatment of genetic diseases. RNA virus-based episomal vector lacking viral glycoprotein gene (ΔG-REVec) is a nontransmissive gene delivery system that enables long-term gene expression i...