Production of high-titer transmission-defective RNA virus-based episomal vector using tangential flow filtration - Komatsu, Yumiko; Kakuya, Yoji; Tomonaga, Keizo (Microbiology and immunology, 2020)
Keywords: Animals; Brain - virology; Cell Line; Chlorocebus aethiops; Female; Filtration - methods; Gene Expression; Gene Transfer Techniques; Genetic Vectors - administration & dosage; Genetic Vectors - isolation & purification; HEK293 Cells; Humans; Plasmids - genetics; Plasmids - isolation & purification; Pregnancy; Rats; Rats, Inbred Lew; RNA Viruses - genetics; RNA Viruses - isolation & purification; Transgenes; Vero Cells; Viral Load; in vivo gene delivery; viral vector; RNA virus-based episomal vector; tangential flow filtration; high-titer