Reversible Post-translational Modification of Proteins by Nitrated Fatty Acids in Vivo

• Published in: The Journal of biological chemistry Vol. 281; no. 29; pp. 20450 - 20463
• Main Authors: Batthyany, Carlos, Schopfer, Francisco J., Baker, Paul R.S., Durán, Rosario, Baker, Laura M.S., Huang, Yingying, Cerveñansky, Carlos, Branchaud, Bruce P., Freeman, Bruce A.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 21.07.2006; American Society for Biochemistry and Molecular Biology
• Subjects: Alkylation; Amino Acid Sequence; Animals; Ascorbic Acid - metabolism; Erythrocytes; Fatty Acids, Nonesterified - metabolism; Glutathione - metabolism; Glyceraldehyde 3-Phosphate - metabolism; Glyceraldehyde-3-Phosphate Dehydrogenases - blood; Glyceraldehyde-3-Phosphate Dehydrogenases - chemistry; Glyceraldehyde-3-Phosphate Dehydrogenases - metabolism; Humans; Kinetics; Mass Spectrometry; Molecular Sequence Data; Nitrates - metabolism; Protein Processing, Post-Translational; Proteins - metabolism; Rabbits; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Nitric oxide (.NO)-derived reactive species nitrate unsaturated fatty acids, yielding nitroalkene derivatives, including the clinically abundant nitrated oleic and linoleic acids. The olefinic nitro group renders these derivatives electrophilic at the carbon β to the nitro group, thus competent for...