Single-Cell RNA Sequencing Identifies Candidate Renal Resident Macrophage Gene Expression Signatures across Species

• Published in: Journal of the American Society of Nephrology Vol. 30; no. 5; pp. 767 - 781
• Main Authors: Zimmerman, Kurt A, Bentley, Melissa R, Lever, Jeremie M, Li, Zhang, Crossman, David K, Song, Cheng Jack, Liu, Shanrun, Crowley, Michael R, George, James F, Mrug, Michal, Yoder, Bradley K
• Format: Journal Article
• Language: English
• Published: United States American Society of Nephrology 01.05.2019
• Subjects: Analysis of Variance; Animals; Antigens, Differentiation, B-Lymphocyte - immunology; Basic Research; Biomarkers - metabolism; Cells, Cultured; Flow Cytometry; Gene Expression Regulation; Histocompatibility Antigens Class II - immunology; Humans; Immunity, Innate - genetics; Intercellular Adhesion Molecule-1 - immunology; Macrophages - metabolism; Male; Mice; Mice, Inbred C57BL; Models, Animal; Parabiosis; Rats; Rats, Sprague-Dawley; Sequence Analysis, RNA; Species Specificity; transcriptional profiling; immunology; macrophages

Resident macrophages regulate homeostatic and disease processes in multiple tissues, including the kidney. Despite having well defined markers to identify these cells in mice, technical limitations have prevented identification of a similar cell type across species. The inability to identify residen...