Metabolic Profiling of Klebsiella oxytoca: Evaluation of Methods for Extraction of Intracellular Metabolites Using UPLC/Q-TOF-MS

• Published in: Applied biochemistry and biotechnology Vol. 167; no. 3; pp. 425 - 438
• Main Authors: Park, Changhun, Yun, Seokhun, Lee, Sang Yup, Park, Kyungmoon, Lee, Jinwon
• Format: Journal Article
• Language: English
• Published: New York Springer-Verlag 01.06.2012; Springer; Springer Nature B.V
• Subjects: Biochemistry; Biological and medical sciences; Biotechnology; Chemical Fractionation - methods; Chemistry; Chemistry and Materials Science; Chloroform; Chromatography, High Pressure Liquid - methods; Ethanol; Extraction processes; Fundamental and applied biological sciences. Psychology; Intracellular Space - metabolism; Klebsiella oxytoca; Klebsiella oxytoca - growth & development; Klebsiella oxytoca - metabolism; Liquid chromatography; Mass spectrometry; Mass Spectrometry - methods; Metabolic disorders; Metabolites; Metabolomics - methods; Methanol; Solvents - chemistry; Metabolic profiling; LC/Q-TOF-MS; Extraction; Intracellular metabolites; Klebsiella oxytoca; Metabolite; Bacteria; Intracellular; Method; Metabolism; Enterobacteriaceae

The global pool of intracellular metabolites is a reflection of all the metabolic functions of an organism. In the absence of in situ methods capable of directly measuring metabolite pools, intracellular metabolite measurements need to be performed after an extraction procedure. In this study, we ev...