Single-Particle Cryo-EM at Crystallographic Resolution

• Published in: Cell Vol. 161; no. 3; pp. 450 - 457
• Main Author: Cheng, Yifan
• Format: Journal Article
• Language: English
• Published: United States 23.04.2015
• Subjects: biochemical compounds; computer software; cryo-electron microscopy; crystallization; Crystallography, X-Ray - instrumentation; Crystallography, X-Ray - methods; Image Processing, Computer-Assisted; Likelihood Functions; Molecular Conformation; Software; X-ray diffraction

Until only a few years ago, single-particle electron cryo-microscopy (cryo-EM) was usually not the first choice for many structural biologists due to its limited resolution in the range of nanometer to subnanometer. Now, this method rivals X-ray crystallography in terms of resolution and can be used...