Whole-transcriptome RNA-seq, gene set enrichment pathway analysis, and exon coverage analysis of two plastid RNA editing mutants

• Published in: Plant signaling & behavior Vol. 12; no. 5; p. e1312242
• Main Authors: Hackett, Justin B., Lu, Yan
• Format: Journal Article
• Language: English
• Published: United States Taylor & Francis 04.05.2017
• Subjects: acetyl-CoA carboxylase; Addendum; Arabidopsis - genetics; Arabidopsis - metabolism; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Arabidopsis thaliana; biosynthesis; DNA, Bacterial - genetics; embryophytes; exon coverage analysis; exons; Exons - genetics; fatty acids; Gene Expression Regulation, Plant; generally applicable gene-set enrichment for pathway analysis; leaves; loss-of-function mutation; messenger RNA; mitochondria; mutants; organelle RNA recognition motif; photosynthetic electron transport; photosystem II; Photosystem II Protein Complex - metabolism; plastid RNA editing; Plastids - genetics; proteins; reverse transcriptase polymerase chain reaction; RNA editing; RNA Editing - genetics; RNA Recognition Motif Proteins - genetics; RNA Recognition Motif Proteins - metabolism; RNA, Plant - genetics; Sanger sequencing; sequence analysis; transfer DNA; wholetranscriptome RNA-seq; Arabidopsis thaliana; generally applicable gene-set enrichment for pathway analysis; organelle RNA recognition motif; Sanger sequencing; wholetranscriptome RNA-seq; exon coverage analysis; plastid RNA editing

In land plants, plastid and mitochondrial RNAs are subject to post-transcriptional C-to-U RNA editing. T-DNA insertions in the ORGANELLE RNA RECOGNITION MOTIF PROTEIN6 gene resulted in reduced photosystem II (PSII) activity and smaller plant and leaf sizes. Exon coverage analysis of the ORRM6 gene s...